CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP031068	Bacillus sp. JAS24-2 chromosome, complete genome	4 crisprs	cas3,csa3,WYL,DinG,DEDDh	1	2	6	0
NZ_CP031070	Bacillus sp. JAS24-2 plasmid pl278, complete sequence	1 crisprs	cas14j,c2c9_V-U4,RT,csa3	1	4	2	1
NZ_CP031069	Bacillus sp. JAS24-2 plasmid pl626, complete sequence	0 crisprs	csa3,cas14j	0	0	2	0

Results visualization

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP031068_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP031068_1

1954450-1954686

Orphan

Consensus_repeat	Method
AGCTTGTTCATCAGCCTGACGTTT	CRT

5 spacers

The CRISPR arrays of NZ_CP031068_1

>merge|NZ_CP031068|1|1954450-1954686|CRT
AGCTTGTTCATCAGCCTGACGTTTTTCATCCTCTTGCTTACGAGCTTGTTCATCAGCCTGACGTTTTTCATTCTCTTGCTTACGAGCTTGTTCATCAGCCTGACGTTTTTCATTCTCTTGCTTACGAGCTTGTTCATCAGCCTGACGTTTTTGTTCTTCTTGTTGTTTGCGAGCTTGTTCATCAGCCTGACGTTTTTCATCCTCTTGCTTACGAGCTTGTTCTTCGGCTAGACGCTT

>NZ_CP031068|1|1|1954450-1954686|CRT
AGCTTGTTCATCAGCCTGACGTTT	TTCATCCTCTTGCTTACG
AGCTTGTTCATCAGCCTGACGTTT	TTCATTCTCTTGCTTACG
AGCTTGTTCATCAGCCTGACGTTT	TTCATTCTCTTGCTTACG
AGCTTGTTCATCAGCCTGACGTTT	TTGTTCTTCTTGTTGTTTGCG
AGCTTGTTCATCAGCCTGACGTTT	TTCATCCTCTTGCTTACG
AGCTTGTTCTTCGGCTAGACGCTT

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP031068.1\|WP_000746918.1\|1950736_1953142_-\|NADPH-nitrite-reductase-large-subunit	unknown	unknown	gnl\|CDD\|224171
NZ_CP031068.1\|WP_000616682.1\|1950403_1950721_-\|nitrite-reductase-small-subunit-NirD	unknown	unknown	gnl\|CDD\|239606
NZ_CP031068.1\|WP_098283103.1\|1948920_1950345_-\|uroporphyrin-III-C-methyltransferase	unknown	unknown	gnl\|CDD\|180864
NZ_CP031068.1\|WP_000536153.1\|1959193_1959742_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|375999
NZ_CP031068.1\|WP_000283853.1\|1961255_1961711_+\|HNH-endonuclease	unknown	unknown	gnl\|CDD\|225724
NZ_CP031068.1\|WP_149216214.1\|1944097_1945267_+\|nitrate-transporter-NarK	unknown	unknown	gnl\|CDD\|225133
NZ_CP031068.1\|WP_000071988.1\|1960848_1961088_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_000541073.1\|1961973_1962321_-\|YolD-like-family-protein	unknown	unknown	gnl\|CDD\|370161
NZ_CP031068.1\|WP_033667098.1\|1945544_1945679_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_087967923.1\|1947747_1948221_-\|precorrin-2-dehydrogenase	unknown	unknown	gnl\|CDD\|180668
NZ_CP031068.1\|WP_000427801.1\|1960352_1960628_+\|stage-V-sporulation-protein-S	unknown	unknown	gnl\|CDD\|377254
NZ_CP031068.1\|WP_087967922.1\|1948213_1948924_-\|sirohydrochlorin-chelatase	unknown	unknown	gnl\|CDD\|225049
NZ_CP031068.1\|WP_149216218.1\|1957438_1959040_+\|hypothetical-protein	unknown	unknown	gnl\|CDD\|224001
NZ_CP031068.1\|WP_149216219.1\|1962511_1963585_+\|tetratricopeptide-repeat-protein	unknown	unknown	gnl\|CDD\|376220
NZ_CP031068.1\|WP_000395679.1\|1955368_1955521_+\|exosporium-protein-ExsG	unknown	unknown	unknown
NZ_CP031068.1\|WP_000824207.1\|1955739_1955949_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_149216216.1\|1953350_1954058_-\|iron-sulfur-cluster-repair-di-iron-protein	unknown	unknown	gnl\|CDD\|274701
NZ_CP031068.1\|WP_000988427.1\|1956045_1956885_-\|acetyltransferase	unknown	unknown	gnl\|CDD\|225073
NZ_CP031068.1\|WP_000043362.1\|1947179_1947557_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_149216215.1\|1945937_1946855_+\|AEC-family-transporter	unknown	unknown	gnl\|CDD\|223751

Protein	Function_ID	Function_description	E-value
NZ_CP031068.1\|WP_000746918.1\|1950736_1953142_-\|NADPH-nitrite-reductase-large-subunit	gnl\|CDD\|224171	COG1251, NirB, NAD(P)H-nitrite reductase [Energy production and conversion].	0
NZ_CP031068.1\|WP_000616682.1\|1950403_1950721_-\|nitrite-reductase-small-subunit-NirD	gnl\|CDD\|239606	cd03530, Rieske_NirD_small_Bacillus, Small subunit of nitrite reductase (NirD) family, Rieske domain; composed of proteins similar to the Bacillus subtilis small subunit of assimilatory nitrite reductase containing a Rieske domain. The Rieske domain is a [2Fe-2S] cluster binding domain involved in electron transfer. Assimilatory nitrate and nitrite reductases convert nitrate through nitrite to ammonium.	2.32497e-55
NZ_CP031068.1\|WP_098283103.1\|1948920_1950345_-\|uroporphyrin-III-C-methyltransferase	gnl\|CDD\|180864	PRK07168, PRK07168, uroporphyrin-III C-methyltransferase.	0
NZ_CP031068.1\|WP_000283853.1\|1961255_1961711_+\|HNH-endonuclease	gnl\|CDD\|225724	COG3183, COG3183, Predicted restriction endonuclease [Defense mechanisms].	7.03093e-09
NZ_CP031068.1\|WP_149216214.1\|1944097_1945267_+\|nitrate-transporter-NarK	gnl\|CDD\|225133	COG2223, NarK, Nitrate/nitrite transporter [Inorganic ion transport and metabolism].	3.49952e-115
NZ_CP031068.1\|WP_000541073.1\|1961973_1962321_-\|YolD-like-family-protein	gnl\|CDD\|370161	pfam08863, YolD, YolD-like protein. Members of this family are functionally uncharacterized. However it has been predicted that these proteins are functionally equivalent to the UmuD subunit of polymerase V from gram-negative bacteria. This family has been shown to belong to the WYL-like superfamily.	1.02382e-27
NZ_CP031068.1\|WP_000988427.1\|1956045_1956885_-\|acetyltransferase	gnl\|CDD\|225073	COG2162, NhoA, Arylamine N-acetyltransferase [Secondary metabolites biosynthesis, transport, and catabolism].	3.12294e-44
NZ_CP031068.1\|WP_000427801.1\|1960352_1960628_+\|stage-V-sporulation-protein-S	gnl\|CDD\|377254	pfam04232, SpoVS, Stage V sporulation protein S (SpoVS). In Bacillus subtilis this protein interferes with sporulation at an early stage and this inhibitory effect is overcome by SpoIIB and SpoVG. SpoVS seems to play a positive role in allowing progression beyond stage V of sporulation. Null mutations in the spoVS gene block sporulation at stage V, impairing the development of heat resistance and coat assembly.	8.22831e-42
NZ_CP031068.1\|WP_087967922.1\|1948213_1948924_-\|sirohydrochlorin-chelatase	gnl\|CDD\|225049	COG2138, COG2138, Sirohydrochlorin ferrochelatase [Inorganic ion transport and metabolism].	6.60314e-38
NZ_CP031068.1\|WP_087967923.1\|1947747_1948221_-\|precorrin-2-dehydrogenase	gnl\|CDD\|180668	PRK06719, PRK06719, precorrin-2 dehydrogenase; Validated.	3.47874e-114
NZ_CP031068.1\|WP_149216216.1\|1953350_1954058_-\|iron-sulfur-cluster-repair-di-iron-protein	gnl\|CDD\|274701	TIGR03652, FeS_repair_RIC, iron-sulfur cluster repair di-iron protein. Members of this protein family, designated variously as YftE, NorA, DrnN, and NipC, are di-iron proteins involved in the repair of iron-sulfur clusters. Previously assigned names reflect pleiotropic effects of damage from NO or other oxidative stress when this protein is mutated. The suggested name now is RIC, for Repair of Iron Centers. [Biosynthesis of cofactors, prosthetic groups, and carriers, Other].	9.86673e-110
NZ_CP031068.1\|WP_149216215.1\|1945937_1946855_+\|AEC-family-transporter	gnl\|CDD\|223751	COG0679, COG0679, Predicted permeases [General function prediction only].	3.44402e-27
NZ_CP031068.1\|WP_149216218.1\|1957438_1959040_+\|hypothetical-protein	gnl\|CDD\|224001	COG1075, LipA, Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold [General function prediction only].	3.60923e-11
NZ_CP031068.1\|WP_149216219.1\|1962511_1963585_+\|tetratricopeptide-repeat-protein	gnl\|CDD\|376220	pfam18801, RapH_N, response regulator aspartate phosphatase H, N terminal. Rap proteins consist of a N-terminal 3-helix bundle and a tetratricopeptide domain. This entry represents the conserved region of the C-terminal bundle.	7.09206e-19
NZ_CP031068.1\|WP_000536153.1\|1959193_1959742_-\|hypothetical-protein	gnl\|CDD\|375999	pfam18573, BclA_C, BclA C-terminal domain. This is the C-terminal domain of BclA (Bacillus collagen-like protein of anthracis) which is expressed on spores of Bacillus species. Trimers of the C-terminal domain (CTD) form the tips of the spore's hair-like nap and are the immunodominant target of vertebrate antibodies and drive trimerization. Structure analysis indicate the C-terminal region of the peptide folding into an all-beta structure with a jelly-fold topology, similar to the first human complement C1q, a member of the tumor necrosis factor (TNF)-like family. The C-terminal globular domain has been shown to be located on the exterior of the exosporium, and therefore is critical in determining the immunogenicity of the spore in a mammalian host.	6.45393e-06

>NZ_CP031068.1|WP_149216216.1|1953350_1954058_-|iron-sulfur-cluster-repair-di-iron-protein
MEHTFTETSIVGEIVTQFPKASDLFKSYRIDFCCGGNKPLIDAIHERNLSATEVLTELNTLYHNTKRLNESEIDWKNASYRELIDYVINKHHRYLNEELPQLSPYVTKVLRVHGASQPHFAQIHKLFHELRMELEQHLIKEEMEDFPLILEFEQNPTNENYTKLRKVVNELENEHNHAGNIIKELRKVTNDFTPPEGACGTYRLVYQRLEALESDLFEHIHLENNILFPRAITKA
>NZ_CP031068.1|WP_000746918.1|1950736_1953142_-|NADPH-nitrite-reductase-large-subunit
MKKRLVMIGNGMAGIRCMEEILKHDSDSYEITIFGDEPHPNYNRIMLSHVLQGKTNIQDIIMNEYSWYEENEITLYTNERVQSINREEKIIITEKKRTLTYDKLIIATGSSAFILPVEGSALSGVTGFRTIEDTQFMIDTAKEKKKAVVIGGGLLGLEAARGLIDLGMDVHVVHLMPSLMEQQLDTKAAALLREDLEAQGMKFLMEKKTVKILGTDHVEGIQFEDGEVVDCDLIVMAVGIRPNTQIAKDAGLIVNRGIVVNDYMLTNDESIYAVGECAEHDGIAYGLVAPLYEQGAILAKHITNLQTDGYSGSIVGTQLKVAGCDLFSAGQIYEDDQTKAISIFNECKRSYKKILIRDNKVVGIVLYGDTADGTRLFSILKKEEDIQEYTPASILHKAGEECELDVATMSADDTICGCNGVTKGTIVHAILEQELKTFEEVKACTKAAGSCGKCRPLVEQVLSHTLGDAFDASAQSTGMCGCTPLSRDEVVAAIHEKGLKSPKEVRNVLGFVHEDGCSKCRPALNYYLRMAIPEEYEDDKSSRFVNERMNGNIQHDGTFSVIPRMYGGVTTADDLMKIAEVAKKYDVPLVKITGASRIGLYGVKKQDLPNVWAELNMASGYAYSKSLRNVKSCVGSRFCRFGTKDSLGLGMLLEQSLEMVDTPHKMKMGVTGCPRNCAEVLTKDFGVVCVENGYQLYIGGNGGTEVREADFVMIVPTEDDVLRIATAYMQYYRETGIYGERTAYWTERLGFDHIKEILQDANMVTKLNERFQKARGTYKEAWGQALETKSLKAMYEVETVK
>NZ_CP031068.1|WP_000616682.1|1950403_1950721_-|nitrite-reductase-small-subunit-NirD
MIQTKEKIKVMRAEDLPIQIGKEVQMKGMSFALFRLSNGDIRAVENRCPHKKGPLAEGIVSGEFVFCPLHDWKISLLTGEVQKPDDGCIQTYEVEVIDGDIYIYM
>NZ_CP031068.1|WP_098283103.1|1948920_1950345_-|uroporphyrin-III-C-methyltransferase
MNGYVYLVGAGPGDEGLITKKAIECLNRADIVLYDRLLNPSFLNYTKETCELIYCGKMPKNHTMRQEMINSHLLQFAKEGKIIVRLKGGDPSIFGRVGEEAETLASANIPYEIVPGITSSIAASIYAGIPLTHRDYSNSVTLLTGHAKGPLTDHGKYNSSYNSDTIAYYMGIKNLPTICENLLQAGKKEDTPVAVIEWGTTGKQRVVTGTLSTIVSIVKNENISNPSMTIVGDVVSLRDQISWKERKPLHGKKVLFASATNKKSAIKQMLQESGAEIYQIPTFKKKEYTLTSEQINMIFTVDRLVFCSADSVDILMQSCSKYHKDIRSLQAELQYMNLSIQEKLMQYGLLSKPAQFSSNSTIYLGRNINRIAVIQEKIGAGSYMMTHAYTIDDRFDEVHSRMLSEFSWGSIVFEGRASIDTFLSEIKRLGFIDILTLPFSYTDVPTLHYANKVGFHHVDEQLQNMIMKKDLVLR
>NZ_CP031068.1|WP_087967922.1|1948213_1948924_-|sirohydrochlorin-chelatase
MKGIIYVGHGSRLQEGNEQFIHFVQSVIKERNERIQKIAFLELTTPTISDAVTETLLEGATEIMIVPVLLFAAAHYKRDIPFEIEQLQKKYPQITFSVVPPFSTHPHMVELVVKRIREAMPMQNSSILLVGRGSSDTQPIHELQQIGAAIERKLGMPVSCSFLTKGTPSFAVELKAITSTASHVYVMPYLLFTGLLLQKIELHTKKYNHVTTCNCLQFDTYMKLTLLERMEECIYV
>NZ_CP031068.1|WP_087967923.1|1947747_1948221_-|precorrin-2-dehydrogenase
MYNMYPLMFNLQNKVVVIIGGGKIAYRKASGLKDTGAFVTVVSPEICEEMKELSYITWKQKAFSNDDIKDAHLIYAATNQHAVNMRVKQAAHDFQWVNVVSDGTESSFHTPGVIRNNDYVVTISTSGKDPSFTKRLKQELTSILPKLIKKISRTHKL
>NZ_CP031068.1|WP_000043362.1|1947179_1947557_+|hypothetical-protein
MSKQNISTIVSGDLIVTHLIGQINTEDVYEWFNDFEQVCQQFISEGRKYKLLVDRKGYTPNHFSVQKVWKDKFFNETILNHSKAIAFLLEEGEILKYLQQSNTKESVEFFDDYEQALIWLNEYPI
>NZ_CP031068.1|WP_149216215.1|1945937_1946855_+|AEC-family-transporter
MFVFIILDVILPILILMLIGAILQRKFQFNLKQLSTLITYCLMPAAVFVNIYDISIETGLLLQIIYYLMLYSLSLIIVSHFISKTLKLEKGESAALKNSISLMNSGNYGLPVSQLIFSHNPVGVSIQIFIVIFQNLLTYSYGIYNLLSATKTIGGIIQSFLRLPVFHALVLGVLFQSFKIQIPNSILLPLNQLANGFVAIALILLGAQLSNIKLNFFHRVITWALIGRLLMGPLLALAVIYILNIDGIVAQSLFIASSFPTSRNTSTIAMEYQIEPELHAQIVLFSTLFSIITVTVVIYLSYILF
>NZ_CP031068.1|WP_033667098.1|1945544_1945679_+|hypothetical-protein
MLGSLSDKYRIDASILRIVTALSDFFRAKFFGKVNGYCEKVYLK
>NZ_CP031068.1|WP_149216214.1|1944097_1945267_+|nitrate-transporter-NarK
MKSPNFQLSLQTSNLIIGFMVWVILSSLMPYIKVDIPLTAGQISIVTAVPVILGSVLRIPIGYWTNRFGARKLFFISFILLLLPVFYISVANSMMDLIIGGLFVGIGGAVFSVGVTSLPKYFPKESHGFVNGIYGVGNAGTAITSFLAPVIATSVGWRTTVQCYLVLLAAFALMNFLLGDRKEKKVNTPLMEQIKGVYKNEKLWFLCIFYFLTFGSFVAFTVYLPNFLVSHFGLEKVDAGMRTAGFIVLATIMRPIGGWLGDKFNPFKILIFVFIGLTLSGIILSFMPSMNVYTFGCLLVAFCAGIGNGTIFKLVPMYFSEQAGIVNGLVSALGGLGGFFPPLILTLLFQLTGHYAIGFMALSEVALACLIITVWMYSQEKLLVMLKNH
>NZ_CP031068.1|WP_000395679.1|1955368_1955521_+|exosporium-protein-ExsG
MEFQLLVTCILQEGNAYFLVTKVDDVITLKVPITAGVAGLFLALGVPRCS
>NZ_CP031068.1|WP_000824207.1|1955739_1955949_+|hypothetical-protein
MKRKTLDIPVTLRREWFLIELAHLTKKYGIEIATSKMDAAPFLRDQVTESKIGKGLQYDKYDDEYIIEN
>NZ_CP031068.1|WP_000988427.1|1956045_1956885_-|acetyltransferase
MMTKLQKEFFKRLEIPAKEITFDDLDEILLKMGMILPYENLDIMAGTIKNISKDNLIEKLLIQKRGGLCYELNSLLYYFLIDCGFQVYKVAGTVYDLYDNKWKPDDGHVIIILSHEDKDYVVDAGFASHLPLHPVPFNGEVISSQTGEYRIRKRNTRKGTHILEMRKGANGESTSFLQSEPSNEWKIGYAFTVDPIDEKKVNKIQKVIVEHKESPFNKGAITCKLTTYGHISLTNKNYTETFKGTKNKRPIESKDYARILRDSFGITQEKYVGKTLERG
>NZ_CP031068.1|WP_149216218.1|1957438_1959040_+|hypothetical-protein
MRLMRRCVALLIVFFIMAPTISTNVQAEVIKELGKGFPDTEVFTPGEWFLGQKPANYDENKPPILFVQGRNGNADSWYGKTVYHDINDMYDYALKAGYQTVFIQLYDAAGKGSASQWDNGKLLAQKLEEIYNHFGKKVNIVAHSKGGIDTQAALVEYGANRFVGNVITLATPHHGSNLADLSYSWWAGWLASILGQKDDGTYSLQIGEMAKFRSIIDNNPAAKLNRYYTATGTSWGPTFSALSMGGLYLSSYGSNDGLVNEWSAKLSYGTHLFTDSRFDHDNIRKGSAVFARIEPYLRTTNVGLPALVASSTSSNEDLEQLNTTSNQNILGGELPQNQWIEQSISVDKKAEGIVSVLTASSDVEVQMVSPKGKIYANKDSAITTGEGESFFNGATIRTFKFDKLEVGEWKIKMMAKQSKDAYLVVSDYKDDAPFVLRMPTKVKVNKPEYKLKKSPVAPEMKGDLSITVRVVNKEGKLVSEFNELQNVNTNTFTGALKDIKQPGVYNVTMDIKGMNKEGKPYNRTIVKSVYVEK
>NZ_CP031068.1|WP_000536153.1|1959193_1959742_-|hypothetical-protein
MGSYYHSSNSYKKCSCKSSSSSNKKCSCDHSHKRSCPAYGTFWQTEFITVPFGDPFPFNHVGPLAGGVSLLNPTTIHFRQAGDYRVSFISSINTTLNPVFPHIPVITIFLNNNPLANSQGDFAIQMNTSSNNECLQLVGETIVTVPANSTLQLRNNSGFNRQSIVTCDNGINSVELTVTKLS
>NZ_CP031068.1|WP_000427801.1|1960352_1960628_+|stage-V-sporulation-protein-S
MENILKVSSKSSPNSVAGAIAGVLRTSGNVEIQAIGAGAINQAIKAIAIARGFVAPSGIDLAFVPAFQEISINNEERTAIKLIVGPRKKRS
>NZ_CP031068.1|WP_000071988.1|1960848_1961088_-|hypothetical-protein
MSNSTSWLDEIIAILTELDGAGTLNQIKTKVMERNKIDLNRYQHEQSIAARIRKTIYQVNYHKNVQWPLLKLNLLNEQF
>NZ_CP031068.1|WP_000283853.1|1961255_1961711_+|HNH-endonuclease
MYQANIDSDFSKVKIAEEEKPENRKKTKMESGREVWPRDPKKAKQAIKQAEFKCEIDDTHETFVSEASRKNYMEAHHLIPLRMQHDFENSLDVVGNIVSICPNCHRLIHYGRDKDKKKVLELLFEQRKDSLKKFGIEVSLKELFGYYGILK
>NZ_CP031068.1|WP_000541073.1|1961973_1962321_-|YolD-like-family-protein
MGYNSFPVVRGRGMVKWNPFASIPEQYEEIHRMFEEQYKVPKPFLTQDTMERIERALMQSFHEEEEIHISYYRDGMVQDMYINVLHIEPMTKTIYCTDAFGLNTKFKFDELVNIN
>NZ_CP031068.1|WP_149216219.1|1962511_1963585_+|tetratricopeptide-repeat-protein
MSVDIISKEEITKLLNEWYQAMISQRVLQSQKIKEDIANKINNIKEDQTILVYYALLNARYKLLIRDMDSTKDILDKIEPLPESTETFLEYYHHLFKAIYAINKANHSEARMQFEKAGTFLEYMHAEIEKAEFNYWLAVHYYHVLKPILAVQFATKANEVFSISPGYELKTAACLNTLGMAHIRLNEFESAEEYLLSALGTFQKSNDELLIKRVKHNLGLLYASQNMPELAIKHLEDSLENNAKTMFLLAREHFKLGYSHIANEYIENGYKISDLCYRHHFRILKAVHNYVPQEELENVVIEGISYFEKEELYDWVKEYASLLGERFYGSENHRKASKYLHIALDADKKSIERRALK

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Crispr_ID: NZ_CP031068_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP031068_2

3041476-3041583

Orphan

Consensus_repeat	Method
TATATCAACGATTTTTTAAATATATC	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP031068_2

>merge|NZ_CP031068|2|3041476-3041583|CRISPRCasFinder
TATATCAACGATTTTTTAAATATATCGTTCATTCCTTTCAATATATCAACGATTTTTCAAATATATCTATCGTAACTTGCAATATATCAACGATTTTTCAAATATATC

>NZ_CP031068|2|1|3041476-3041583|CRISPRCasFinder
TATATCAACGATTTTTTAAATATATC	GTTCATTCCTTTCAATATATCAACGATTTTTCAAATATATCTATCGTAACTTGCAA
TATATCAACGATTTTTCAAATATATC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP031068.1\|WP_000477749.1\|3048567_3049632_-\|ABC-transporter-permease	unknown	unknown	gnl\|CDD\|223650
NZ_CP031068.1\|WP_000636458.1\|3028404_3029355_-\|class-A-beta-lactamase	unknown	unknown	gnl\|CDD\|185339
NZ_CP031068.1\|WP_098434882.1\|3043271_3044231_-\|RNA-splicing-ligase-RtcB	unknown	unknown	gnl\|CDD\|376465
NZ_CP031068.1\|WP_149216759.1\|3025637_3027425_+\|response-regulator	unknown	unknown	gnl\|CDD\|225107
NZ_CP031068.1\|WP_149216466.1\|3050510_3051770_+\|MFS-transporter	unknown	unknown	gnl\|CDD\|340863
NZ_CP031068.1\|WP_000505587.1\|3027963_3028323_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_149216464.1\|3038773_3039367_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_000803871.1\|3049817_3050441_+\|ArsR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|238042
NZ_CP031068.1\|WP_000005138.1\|3036867_3038346_-\|DHA2-family-efflux-MFS-transporter-permease-subunit	unknown	unknown	gnl\|CDD\|129794
NZ_CP031068.1\|WP_000851006.1\|3046735_3047848_+\|LPXTG-cell-wall-anchor-domain-containing-protein	unknown	unknown	gnl\|CDD\|274009
NZ_CP031068.1\|WP_000022538.1\|3029661_3030090_-\|DUF5412-domain-containing-protein	unknown	unknown	gnl\|CDD\|375187
NZ_CP031068.1\|WP_000202597.1\|3047896_3048568_-\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|224059
NZ_CP031068.1\|WP_061654911.1\|3044513_3044732_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|376465
NZ_CP031068.1\|WP_140160684.1\|3045992_3046595_+\|AAA-family-ATPase	unknown	unknown	gnl\|CDD\|379324
NZ_CP031068.1\|WP_061654910.1\|3041711_3042944_-\|DUF4073-domain-containing-protein	unknown	unknown	gnl\|CDD\|290024
NZ_CP031068.1\|WP_097796241.1\|3044868_3045789_-\|class-I-SAM-dependent-methyltransferase	unknown	unknown	gnl\|CDD\|377216
NZ_CP031068.1\|WP_098329880.1\|3033086_3036284_-\|bifunctional-P-450/NADPH--P450-reductase	unknown	unknown	gnl\|CDD\|223446
NZ_CP031068.1\|WP_149216465.1\|3039497_3040058_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_001046619.1\|3040318_3041314_-\|D-cysteine-desulfhydrase	unknown	unknown	gnl\|CDD\|179673
NZ_CP031068.1\|WP_149216463.1\|3030763_3032875_+\|TerD-family-protein	unknown	unknown	gnl\|CDD\|132992

Protein	Function_ID	Function_description	E-value
NZ_CP031068.1\|WP_000477749.1\|3048567_3049632_-\|ABC-transporter-permease	gnl\|CDD\|223650	COG0577, SalY, ABC-type antimicrobial peptide transport system, permease component [Defense mechanisms].	9.56957e-10
NZ_CP031068.1\|WP_000022538.1\|3029661_3030090_-\|DUF5412-domain-containing-protein	gnl\|CDD\|375187	pfam17428, DUF5412, Family of unknown function (DUF5412). This is a family of unknown function found in Bacteria. Members of this family have one or two predicted trans-membrane regions.	1.62801e-53
NZ_CP031068.1\|WP_000636458.1\|3028404_3029355_-\|class-A-beta-lactamase	gnl\|CDD\|185339	PRK15442, PRK15442, beta-lactamase TEM; Provisional.	5.40077e-63
NZ_CP031068.1\|WP_149216759.1\|3025637_3027425_+\|response-regulator	gnl\|CDD\|225107	COG2197, CitB, Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain [Signal transduction mechanisms / Transcription].	8.82279e-68
NZ_CP031068.1\|WP_098434882.1\|3043271_3044231_-\|RNA-splicing-ligase-RtcB	gnl\|CDD\|376465	pfam01139, RtcB, tRNA-splicing ligase RtcB. This family of RNA ligases (EC:6.5.1.3) join 2',3'-cyclic phosphate and 5'-OH ends. They catalyze the splicing of tRNA and may also participate in tRNA repair and recovery from stress-induced RNA damage.	1.1017e-58
NZ_CP031068.1\|WP_149216466.1\|3050510_3051770_+\|MFS-transporter	gnl\|CDD\|340863	cd06173, MFS_MefA_like, Macrolide efflux protein A and similar proteins of the Major Facilitator Superfamily of transporters. This family is composed of Streptococcus pyogenes macrolide efflux protein A (MefA) and similar transporters, many of which remain uncharacterized. Some members may be multidrug resistance (MDR) transporters, which are drug/H+ antiporters (DHAs) that mediate the efflux of a variety of drugs and toxic compounds, conferring resistance to these compounds. MefA confers resistance to 14-membered macrolides including erythromycin and to 15-membered macrolides. It functions as an efflux pump to regulate intracellular macrolide levels. The MefA-like family belongs to the Major Facilitator Superfamily (MFS) of membrane transport proteins, which are thought to function through a single substrate binding site, alternating-access mechanism involving a rocker-switch type of movement.	4.58801e-61
NZ_CP031068.1\|WP_000803871.1\|3049817_3050441_+\|ArsR-family-transcriptional-regulator	gnl\|CDD\|238042	cd00090, HTH_ARSR, Arsenical Resistance Operon Repressor and similar prokaryotic, metal regulated homodimeric repressors. ARSR subfamily of helix-turn-helix bacterial transcription regulatory proteins (winged helix topology). Includes several proteins that appear to dissociate from DNA in the presence of metal ions.	4.9769e-11
NZ_CP031068.1\|WP_001046619.1\|3040318_3041314_-\|D-cysteine-desulfhydrase	gnl\|CDD\|179673	PRK03910, PRK03910, D-cysteine desulfhydrase; Validated.	0
NZ_CP031068.1\|WP_000005138.1\|3036867_3038346_-\|DHA2-family-efflux-MFS-transporter-permease-subunit	gnl\|CDD\|129794	TIGR00711, Uncharacterized_MFS-type_transporter_YhcA, drug resistance transporter, EmrB/QacA subfamily. This subfamily of drug efflux proteins, a part of the major faciliator family, is predicted to have 14 potential membrane-spanning regions. Members with known activities include EmrB (multiple drug resistance efflux pump) in E. coli, FarB (antibacterial fatty acid resistance) in Neisseria gonorrhoeae, TcmA (tetracenomycin C resistance) in Streptomyces glaucescens, etc. In most cases, the efflux pump is described as having a second component encoded in the same operon, such as EmrA of E. coli. [Cellular processes, Toxin production and resistance, Transport and binding proteins, Other].	6.57844e-109
NZ_CP031068.1\|WP_000851006.1\|3046735_3047848_+\|LPXTG-cell-wall-anchor-domain-containing-protein	gnl\|CDD\|274009	TIGR02169, chromosome_segregation_protein_related_ptotein, chromosome segregation protein SMC, primarily archaeal type. SMC (structural maintenance of chromosomes) proteins bind DNA and act in organizing and segregating chromosomes for partition. SMC proteins are found in bacteria, archaea, and eukaryotes. It is found in a single copy and is homodimeric in prokaryotes, but six paralogs (excluded from this family) are found in eukarotes, where SMC proteins are heterodimeric. This family represents the SMC protein of archaea and a few bacteria (Aquifex, Synechocystis, etc); the SMC of other bacteria is described by TIGR02168. The N- and C-terminal domains of this protein are well conserved, but the central hinge region is skewed in composition and highly divergent. [Cellular processes, Cell division, DNA metabolism, Chromosome-associated proteins].	3.0063e-13
NZ_CP031068.1\|WP_097796241.1\|3044868_3045789_-\|class-I-SAM-dependent-methyltransferase	gnl\|CDD\|377216	pfam04072, LCM, Leucine carboxyl methyltransferase. Family of leucine carboxyl methyltransferases EC:2.1.1.-. This family may need divides a the full alignment contains a significantly shorter mouse sequence.	1.90793e-61
NZ_CP031068.1\|WP_000202597.1\|3047896_3048568_-\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|224059	COG1136, SalX, ABC-type antimicrobial peptide transport system, ATPase component [Defense mechanisms].	5.4485e-104
NZ_CP031068.1\|WP_061654911.1\|3044513_3044732_-\|hypothetical-protein	gnl\|CDD\|376465	pfam01139, RtcB, tRNA-splicing ligase RtcB. This family of RNA ligases (EC:6.5.1.3) join 2',3'-cyclic phosphate and 5'-OH ends. They catalyze the splicing of tRNA and may also participate in tRNA repair and recovery from stress-induced RNA damage.	2.25316e-05
NZ_CP031068.1\|WP_061654910.1\|3041711_3042944_-\|DUF4073-domain-containing-protein	gnl\|CDD\|290024	pfam13285, DUF4073, Domain of unknown function (DUF4073). This family is frequently found at the C-terminus of bacterial proteins carrying the family, Metallophos pfam00149.	2.32434e-101
NZ_CP031068.1\|WP_098329880.1\|3033086_3036284_-\|bifunctional-P-450/NADPH--P450-reductase	gnl\|CDD\|223446	COG0369, CysJ, Sulfite reductase, alpha subunit (flavoprotein) [Inorganic ion transport and metabolism].	1.59264e-171
NZ_CP031068.1\|WP_140160684.1\|3045992_3046595_+\|AAA-family-ATPase	gnl\|CDD\|379324	pfam13671, AAA_33, AAA domain. This family of domains contain only a P-loop motif, that is characteristic of the AAA superfamily. Many of the proteins in this family are just short fragments so there is no Walker B motif.	1.15153e-10
NZ_CP031068.1\|WP_149216463.1\|3030763_3032875_+\|TerD-family-protein	gnl\|CDD\|132992	cd06974, TerD_like, Uncharacterized proteins involved in stress response, similar to tellurium resistance terD. Tellurium resistance terD like proteins. This family is composed of uncharacterized proteins involved in stress response, such as the tellurium resistance proteins, chemical-damaging agent resistance proteins, and general stress proteins from a variety of organisms. The tellurium resistance proteins are homologous terA,-D,-E,-F,-Z,-X gene products, which confer tellurium resistance mediated by plasmids. Currently, the biochemical mechanism of tellurium resistance remains unknown. The family also contains several ter gene homologues, YceC, YceD, YceE, for which there is no clear evidence for any involvement in the tellurium resistance. A putative cAMP-binding protin CABP1 shows a significant similarity to the terD protein and is also included in this family.	2.21892e-15

>NZ_CP031068.1|WP_001046619.1|3040318_3041314_-|D-cysteine-desulfhydrase
MNLAKFPRKKYTESYTPIEKLNNFSEALGGPTIYFKRDDLLGLTAGGNKTRKLEFLVADAQENGADTLITAGGIQSNHCRLTLAAAVKEKMKCILVLEEGLEPEEKRDFNGNYFLYHLLGAENVIVVPNGADLMEEMHKVAQEVSEKGNTPYVIPVGGSNPTGAMGYVACAQEIMAQSFEQGIDFSSVVCVSGSGGMHAGLVTGFSGTQSHIPVIGINVSRGKAEQEEKVAKLVDETSAHVGIPNFISRDAVTCFDEYVGPGYALPTPEMVEAVQLLAKTEGILLDPVYTGKAVAGLIDLIRKDKFNKEDNILFVHSGGSPALYANTSLFA
>NZ_CP031068.1|WP_149216465.1|3039497_3040058_-|hypothetical-protein
MRAKKLVTLAVPFMLLVGCEVEDKDSTHKTEQTSKAITKTVISEQQYPYYICEQVVEFQFKKDELLLNLGESLKDKEKAKAKAVLKTSNEIDKILDNMEHIKVPDTYKDIHKSIQEGVTEARKATKLIKDAGKEDKQKIQEATMKGTEFLSGVDGEHWKKAIYELSQENKDAYSKALDKKVKEHSK
>NZ_CP031068.1|WP_149216464.1|3038773_3039367_+|hypothetical-protein
MDQYWYLNSKGNGEQYTNQISLYSQPEITTSEVMNDLKIPENWSYRVYPVSWDSSANYTGFNPHPPLFSWGQPINYAGIMPQGHPANWTPLPSHAGFNFQKPPFPWAQPFNHTGMFAQGHLAALLSPPAKAFHFNTNNSGVFGNPPGWGGPWAPMGWGNNGGNGQLGMLGNLLTAGKGTMSGIGMISSLIGMGKFFF
>NZ_CP031068.1|WP_000005138.1|3036867_3038346_-|DHA2-family-efflux-MFS-transporter-permease-subunit
MSETLHKRKPPYVMLVILFIGAFVSFLNNSLLNVALPSIMKDLNIQDYSTIQWLSTGYMLVSGILIPASAFLITRFSNRSLFITSMVIFILGTALAAVAPNFGLLLTGRMVQAAGSSVMGPLLMNIMLVSFPREKRGTAMGIFGLVMITAPAIGPTLSGYIVEYYDWRLLFEMILPLAIISLLLGIWKSENVMKQNKNAKLDYLSLLLSSVGFGGLLYGFSSASSDGWTNKVVLTTLIIGAIALIAFIIRQLKMDEPLLDLRVYKYPMFALASVIAIVNAVAMFSGMILTPAYVQNVRGISPLSSGLMMLPGAVIMGIMSPITGKLFDKYGPRILGIVGLSITAVSTYMLASLQIDSSHTHIILIYTLRMFGMAMVMMPLMTNGLNQLPTRLNPHGTAVNNTAQQVSGSIGTAILVTIMNSVTKTKAESLMSHIDPTTFTETTKAIVTQKALLSGIQYSFYVALGMNVVALLLVFFVKRVDTSAEAVEKLNR
>NZ_CP031068.1|WP_098329880.1|3033086_3036284_-|bifunctional-P-450/NADPH--P450-reductase
MEKKVSAIPQPKTYGPLGNLPLIDKDKPTLSFIKIAEEYGPIFQIQTLSDTIIVVSGHELVAEVCDETRFDKSIEGALAKVRAFAGDGLFTSETHEPNWKKAHNILMPTFSQRAMKDYHAMMVDIAVQLVQKWARLNPNENVDVPEDMTRLTLDTIGLCGFNYRFNSFYRETPHPFITSMTRALDEAMHQLQRLDIEDKLMWRTKRQFQHDIQSMFSLVDNIIAERKSSGDQEENDLLSRMLNVSDPETGEKLDDENIRFQIITFLIAGHETTSGLLSFAIYFLLKNPDKLKKAYEEVDRVLTDPTPTYQQVMKLKYIRMILNESLRLWPTAPAFSLYAKEDTVIGGKYPIKKGEDRISVLIPQLHRDKDAWGDNVEEFQPERFEELDKVPHHAYKPFGNGQRACIGMQFALHEATLVMGMLLQHFELIDYQNYQLDVKQTLTLKPGDFKIRILPRKQTISHPTALAPTEDKLKNDEIKQHVQKTPSIIGADNLSLLVLYGSDTGVAEGIARELADTASLEGVQTEVVALNDRIGSLPKEGAVLIVTSSYNGKPPSNAGQFVQWLEELKPDELKGVQYAVFGCGDHNWASTYQRIPRYIDEQMAQKGATRFSKRGEADASGDFEEQLEQWKQSMWSDAMKAFGLELNKNMEKERSTLSLQFVSRLGGSPLARTYEAVYASVLENRELQSSNSDRSTRHIEVSLPEGATYKEGDHLGVLPVNSEKNINRILRRFGLNGKDQVILSASGRSINHIPLDSPVSLLDLLSYSVEVQEAATRAQIREMVTFTACPPHKKELEALLEEGVYHEQILKKRISMLDLLEKYEACEIRFERFLELLPALKPRYYSISSSPLVVQNRLSITVGVVNAPAWSGEGTYEGVASNYLAQRHNKDEIICFIRTPQSNFELPKDPETPIIMVGPGTGIAPFRGFLQARRVQKQKGINLGEAHLYFGCRHPEKDYLYRTELENDERDGLISLHTAFSRLEGHPKTYVQHLIKQDRINLISLLDNGAHLYICGDGSKMAPDVEDTLCQAYQEIHEVSEQEARNWLDRVQDEGRYGKDVWAGI
>NZ_CP031068.1|WP_149216463.1|3030763_3032875_+|TerD-family-protein
MKNSIYIRRSLKVIIKREENKLPNIYLATVLKKLENLGFTFSEPLIEELQTLSVDAFTSFYKELVKHLKEMVGAHIQFTPMYPNFPQQMMDLSDADLYINAIIHYVTLRLPVSKVEERLPLLDRVDLKVIDLGSEEDFNQMISQLISANSSISSTDKTDVEWAITHTEDVSCFLPNVIPHKENMSFIIGVLLINRKISADAAAKYFKTATDVLRLAVALSEGDVSLASSVRFKKFNRAERRFLLGLLEQCGNITEDMLRYKKRWIRLGEILHPAEYHTRFPKTHRAFEILRNNIKVETFNGKIEATLLNRDIMTAKNLLKTRPGEFARRLDHLIRLCSDKSTDVFDILEDFLSIIGNVSTPVLLQLTAHFKHRNDKNEFRTFFPKGNVAKAIGIENTLPFISEDICLMIVKMCEDALKNRFAELPSLGKVFLDEQLKNHLVPFSQRSASKALRTLSRGSKVDLPEGDTIRFFLWWKEGYVNGQHTGRVDIDLSAAMYDEDWQYKEHVSFTNLRSKNFKAYHSGDITSAPKGASEFIDFDIPSVLKYGARYVVMTLLSYTDQPYKDLPECFTGWMVRQYPGSGEIFEPSTVQDKVDITADTQISIPVILDLKERKLIWTDLSLTRDLTYDNTIEANQKGMILVGKALTNLVKPNLYDLFRLHIEARGELVQDIEEAESIFSLDKGITPFDIEKIISDFIADSKG
>NZ_CP031068.1|WP_000022538.1|3029661_3030090_-|DUF5412-domain-containing-protein
MSIINTKGEIKKTKRKVLLTILTMLVIIIGFGYWKLFSLQGVPKGEFIRTVKSPDGKYLIKTYFHNAGSLSADAVRGELVNLDTDSEKNIYWNYPDTDPYIEWVNKNIVRIGDQTLDVSQKETYDWRDDDKHVKEMPKQFIR
>NZ_CP031068.1|WP_000636458.1|3028404_3029355_-|class-A-beta-lactamase
MIVLNKFFNILHYKKMVPVVFLSCATLIGCSDGNTQSELPKQTKQTNQIKQEITGNDSFVKLEKEFDAKLGIYALDTDTNQTVTYQSDERFAYASTHKALAVGVLLQKKSIEDLNQRVLYTREDLVNYNPITEKYVDSGMTLKELADASLRYSDNTAQNLILKQLGGPSEFKKSLREVGDTVTNPERFEPELNEVPPGDVRDTSTPKALATSLQAFTLGDVLSTEKRDLLIDWMKRNTTGDNLIRAGVPGGWEVADKTGSGSYGTRNDIAIIWPPNKKPIVLAILSNHDKEDAKYDDKLIAEATKIVLDALKVTNK
>NZ_CP031068.1|WP_000505587.1|3027963_3028323_-|hypothetical-protein
MGFFVGVAFLATMASCLMFKVLSGASKDVARYQTFRKIVVRRELLTNSIYKELYFIFLFPAIVGITHILFGMNMFSPILINPYSRIWLPLVIFVVIYSIYYLITVQLYKGIVLSKKRSM
>NZ_CP031068.1|WP_149216759.1|3025637_3027425_+|response-regulator
MLDMVKYWFWYDWIMLGVRILVSVSIVVITLDFQNSLTLPLWIIIFWEVVAFSIPWVALLFNYKYYLFTEILLYGGLCIYLTSLFPEANKTFLISVFLIAANSKHLSYYWTAPITVFITTGIFYALAPSNNYWIMVTYYGLAYVMGFAFHLLIVNHKQNEAIRKQNTVLEQYMSQIERITLAEERNRLSSELHDTVGHAYTSIIMGMETLRTELATEMGIQRLDSLLEMGRKSIEDVRGYLHQMNSPCQSSSLIQSLQNLGAEFQEHAQVNVSFRTFGEEYQLSRQAKIAFVRCLQESLTNAVRHGQGTEIIVSLQFEQQYTRLEVQDNGKGNVEWQEGFGMNAMKERAMNLQGHLSVYTKPEEGMLVTCTIPRQTEIKDGLIRLLIVDDQPFVRESLRTLLDRYEDLNVVGLAEDGNQAIDLCGRLQPHVILMDLDMQHMDGIEATKKIKQQWPHIRVLIFTTFQDTEQAVESLRNGADGFLLKSIETLELANTIRLIHKGGTLIDQGMSHKIFEKFDEQKETPQSKAAAYELTAREIEILQLVAKGLRYTTIASRLYLSNGTVRNYASTAYTKLGVSNKEEAVQKALEIGIIE
>NZ_CP031068.1|WP_061654910.1|3041711_3042944_-|DUF4073-domain-containing-protein
MKKTVASLAVMAALLPTFSAHAEGKEQVRKSATTFNVISDIQGDLGDFDHVLKDMNKVTPLSRALIMNGDITPTGQQSQYDDVKRVLNKNKHPENVWSTVGNHEFYAGKWTADGKLSQSTWPNGVTEETLFNRYLKFSGQEKVYHKKELDGYPLLFLGTEKYMKYHDSKMWDEVYMSDEQLGWLKQNLEEYSQKDKNKPIFIFSHHVLPDTVSGSRQSPYLQDYLNVDKLYDILKDYPQVVFFTSHTHWDLNLPDWAGKKKIAGGDKKGFTVVNTGGIETGWMSAGPNGGEKTAPDGYSFKQGLQVKAYGNDVIVTAYDYKRDKDIKKLLISDSKIAQMAPNVTADDSENIIVGATEYMEYSIEGTNEWHTYSKANPPKFDGDKVVYVRHKGEMNLEPGLTQLLRFSVNK
>NZ_CP031068.1|WP_098434882.1|3043271_3044231_-|RNA-splicing-ligase-RtcB
MEVVIINKKKEEINFDYLDETIRKFVPSGFRIRDKEHRFSKMIDFDSVRAPFTLQRAQKSIGTLGGGNHFVELNEDDEGNVYIVIHSGSRNLGKQVAEYYQNFAYEQLINVKSIKEEIIERLTKEGRQQEIHEALREIKKPVICKELAYLEGQGFKDYMNDMKIAQKYAELNRKAMIDEIVTTMDWKVTDQFTTIHNYIDMDNMILRKGAISAQKDERVIIPINMRDGSIIAFGKGNPDWNFSGPHGAGRIMSRKKAKEVLNLEDFQNTMTEVWTTSVAESTLDEAPMVYKPMDEIIENTKETIDIKHIIKPLYNFKAN
>NZ_CP031068.1|WP_061654911.1|3044513_3044732_-|hypothetical-protein
MLKLRGKYNEAKVYTTNVEKMAAGQIIDLCNQEFVKDSKIRIMPDTHAGAGCTIGTTMTVQDKIVPNLVGVN
>NZ_CP031068.1|WP_097796241.1|3044868_3045789_-|class-I-SAM-dependent-methyltransferase
MCVKQGEASVTSLVSAFSRAYHSEFDNPKIFDDYVAKEFISPKERINIETNMVQGIHFFNKEIAEQFQDNPKEILKWITQVQLSPTPLARAAYCERVLLHEITLGAKQYVILGAGLDTFSFRHRELENKIEIFEVDHPSTQAFKKERIKEVEIEVPNNLHFVSMDFTKGFFYEQLRNEGFENKKTFFSLLGVTYYLTKEELASLLECLFEMVPAGSSIVFDYPDENLFTEKGLSNRVENMVKMAAVGGEPMKSCFSYAEMEALFEKAGLLIYEHLSPEDINKLYFEGRNDYLKAFETVHYVHAVKR
>NZ_CP031068.1|WP_140160684.1|3045992_3046595_+|AAA-family-ATPase
MEANMKRFVIITVGKTHSGKTTFAKALEKELPHSFVMDQDNQAEFINTHYEKLQPAEGANTFKHGLSKFIVDYAKEHTNLHLIICNSNRSKSGRFYLLNELFPQNEYVRILVHFDIPDDVLYERVARSTRSTNIFRGGYSSFKEVLDRQQTESLHDDVVDPVENEADYLFVIRNNKDVSFTIEEIVHLAKGLSPTPNKRL
>NZ_CP031068.1|WP_000851006.1|3046735_3047848_+|LPXTG-cell-wall-anchor-domain-containing-protein
MKTKQTIAASTLALAMIAGANSAHAEVNDATPQQSTGDRLAEIKQHKQELDAKLQQHKENVDQTLNELNKVKENVDTKVNELHERKQVADEKINEVKQHKQELDAKLQQDKQIAEDKIAEIKEHKKQVEDKVAEVKEHKQNIDNKVNEIKEHKQTVDEKVNEMKQHKENIDQKVNELKEVKKQVDEKLAELKKAKQTAEDKLAELKENKPNTGNTLEELKKIKGNLDSLSANLELAKQDVKNKLAVLQEARQDLINKINEIKQSKQTVSDDLSKKKQDLDIKINDFKHTEKKIDDKLAELHTTKQNVDNKINEVSQSKQMQADNGTKNNNAKELPNAGSEQSNNMALGMLSVLGGVLLLTRNKIKTLFSK
>NZ_CP031068.1|WP_000202597.1|3047896_3048568_-|ABC-transporter-ATP-binding-protein
MTSLLKLDKVSKVYGEGNTEVTALHPISLDVKAGEFIGIVGPSGSGKSTLLSIAGALLSPSKGDIYIREQNITQLSEKEMTDIRLKKIGFIFQFANLVPYLNVKEQLLYIAKLKKESKQESEKRADHLLAAFGLGERKNHYPNQLSGGEKQRVAIARAFMNNPDLILADEPTASLDSKRAREVVEMMKREVKESQKAAIMITHDERMLDVCDRILTLRDGQLI
>NZ_CP031068.1|WP_000477749.1|3048567_3049632_-|ABC-transporter-permease
MFLALRELKQSKLRYGLIGLIMVLLSFLVLVISGLANGLSYDNASSIQNMEANKFVLADDAENKLLRSQIKKDDVDNVVNQVGEKEAVPFRVKVSTYEKKDSSKKVDIAIFSSERDSFLEPKIVKGEKLGTANNEMVADESIKEKGIDIGDTIIDPVSKKEFTIVGFTKDQMFSHTPVVYVNEDVWGAISQPNQKDYSAIALNTDKEIKNAHVIDKKEVLQSIPGFKEEQGTLTMIIAFLLVIAALLIGVFFYVITLQKTQQLGVLKAIGTKNSYLANSLVVQALFLSVVAMVISIVLVQGLEQILPAGMPFLLTTPMIAQYAAIFIVISILGTLISLYQVLKVDALEAIGGGM
>NZ_CP031068.1|WP_000803871.1|3049817_3050441_+|ArsR-family-transcriptional-regulator
MKPMLTLTTYSQLKAISDPLRVEMMMRLCERPYTGQLLSEKFGIPRAKIHYHLKELEKNGFIEIVYTEEKNGIIQKFYQSVARGFTPAADLLPHLEMLSESGRQIFLQMAETTKNHILAAPEEAFILRKESEDPAEWNYVSSCWEFDATPEQFQVWVKKFHELMAELNDIAKDADKDPNSKSYYISTTALQIDERAMQRFVKKEEKS
>NZ_CP031068.1|WP_149216466.1|3050510_3051770_+|MFS-transporter
MDILKNRNFLLLFLGRIFTNIGDSLYYVAAMWLVYKLSGNPFYSGLAGFLTLLPSVLQFLTGPFVDRWSIKNTLVLTQVLQCILILIIPITHYFDLLTVQLLLIIMPIVAFIEQFAYPAQSKALPLLLHKTQLLKGNSLFSFAYQGIDLICTTISGILVALLGAITLYVIDSFTFAITALLFFSLKMPKQTETGTSLSTKQYFSDLKEGFSIVFRSLMGAFLIGSVVANFSIGMTMAILPSFADSLGGVKSYGFFLAAISAGSLIGALFSSWVGKRNVGRTSIISFATGAIFWFLSTIVPFQWLSIFLFGLAWIPIGATNILFATISQIVIPNQYIGRINSVMRSMGTIAMPFGSLIGGYTANVFSSQLIFALASIGIFFISLVWLLHPKLRALPKADEITADTFGVRFKEERGKGAAL

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Crispr_ID: NZ_CP031068_3

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP031068_3

4740349-4740473

Orphan

Consensus_repeat	Method
CTTAAACAAGCGTTTGATTAATTCTCCATTTTTCTTTCTTAACC	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP031068_3

>merge|NZ_CP031068|3|4740349-4740473|CRISPRCasFinder
CTTAAACAAGCGTTTGATTAATTCTCCATTTTTCTTTCTTAACCTTTGAACTACAGGAGTTTCTTCCTACCGAACATACAGCTTAAACAAACGTTTGATTAATTCTCCATTTTTCTTTCTTAACC

>NZ_CP031068|3|2|4740349-4740473|CRISPRCasFinder
CTTAAACAAGCGTTTGATTAATTCTCCATTTTTCTTTCTTAACC	TTTGAACTACAGGAGTTTCTTCCTACCGAACATACAG
CTTAAACAAACGTTTGATTAATTCTCCATTTTTCTTTCTTAACC

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP031068.1\|WP_149216684.1\|4746504_4747227_-\|MBL-fold-metallo-hydrolase	unknown	unknown	gnl\|CDD\|293807
NZ_CP031068.1\|WP_065702912.1\|4744774_4745488_-\|CPBP-family-intramembrane-metalloprotease	unknown	unknown	gnl\|CDD\|376806
NZ_CP031068.1\|WP_000640870.1\|4738641_4738986_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|224572
NZ_CP031068.1\|WP_000568176.1\|4738329_4738629_-\|thioredoxin-family-protein	unknown	unknown	gnl\|CDD\|239245
NZ_CP031068.1\|WP_000601778.1\|4736395_4737421_-\|methionine-ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|224058
NZ_CP031068.1\|WP_000713771.1\|4741235_4741883_+\|phosphatase-PAP2-family-protein	unknown	unknown	gnl\|CDD\|239481
NZ_CP031068.1\|WP_001002987.1\|4744180_4744429_-\|spore-coat-protein	unknown	unknown	gnl\|CDD\|377935
NZ_CP031068.1\|WP_141403578.1\|4742984_4744019_-\|L-lactate-dehydrogenase	unknown	unknown	gnl\|CDD\|133427
NZ_CP031068.1\|WP_000218968.1\|4739787_4740153_-\|arsenate-reductase-family-protein	unknown	unknown	gnl\|CDD\|239334
NZ_CP031068.1\|WP_000416291.1\|4747366_4749151_-\|acyl-CoA-dehydrogenase	unknown	unknown	gnl\|CDD\|173850
NZ_CP031068.1\|WP_000722399.1\|4734026_4734833_-\|MetQ/NlpA-family-ABC-transporter-substrate-binding-protein	unknown	unknown	gnl\|CDD\|224381
NZ_CP031068.1\|WP_000026896.1\|4739362_4739746_-\|glycine-cleavage-system-protein-GcvH	unknown	unknown	gnl\|CDD\|234918
NZ_CP031068.1\|WP_000359401.1\|4735737_4736403_-\|ABC-transporter-permease	unknown	unknown	gnl\|CDD\|224922
NZ_CP031068.1\|WP_000826143.1\|4734902_4735715_-\|MetQ/NlpA-family-ABC-transporter-substrate-binding-protein	unknown	unknown	gnl\|CDD\|224381
NZ_CP031068.1\|WP_000666172.1\|4741948_4742962_-\|PTS-sugar-transporter-subunit-IIC	unknown	unknown	gnl\|CDD\|379113
NZ_CP031068.1\|WP_000826869.1\|4740564_4741092_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_001242139.1\|4737832_4738177_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_098294023.1\|4745952_4746501_-\|TetR/AcrR-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|379115
NZ_CP031068.1\|WP_001180551.1\|4744442_4744628_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_000929160.1\|4733002_4733788_-\|Fe-S-cluster-assembly-ATPase-SufC	unknown	unknown	gnl\|CDD\|223473

Protein	Function_ID	Function_description	E-value
NZ_CP031068.1\|WP_149216684.1\|4746504_4747227_-\|MBL-fold-metallo-hydrolase	gnl\|CDD\|293807	cd07721, yflN-like_MBL-fold, uncharacterized subgroup which includes Bacillus subtilis yflN; MBL-fold metallo hydrolase domain. This subgroup includes the uncharacterized Bacillus subtilis yflN protein. Members of this subgroup belong to the MBL-fold metallo-hydrolase superfamily which is comprised mainly of hydrolytic enzymes which carry out a variety of biological functions. The class B metal beta-lactamases (MBLs) from which this fold was named are only a small fraction of the activities which are included in this superfamily. Activities carried out by superfamily members include class B beta-lactamases, hydroxyacylglutathione hydrolases, AHL (acyl homoserine lactone) lactonases, persulfide dioxygenases, flavodiiron proteins, cleavage and polyadenylation specificity factors such as the Int9 and Int11 subunits of Integrator, Sdsa1-like and AtsA-like arylsulfatases, 5'-exonucleases human SNM1A and yeast Pso2p, ribonuclease J and ribonuclease Z, cyclic nucleotide phosphodiesterases, insecticide hydrolases, and proteins required for natural transformation competence. Classical members of the superfamily are di-, or less commonly mono-, zinc-ion-dependent hydrolases, however the diversity of biological roles is reflected in variations in the active site metallo-chemistry.	3.80714e-72
NZ_CP031068.1\|WP_000640870.1\|4738641_4738986_-\|hypothetical-protein	gnl\|CDD\|224572	COG1658, COG1658, Small primase-like proteins (Toprim domain) [DNA replication, recombination, and repair].	2.03274e-21
NZ_CP031068.1\|WP_000568176.1\|4738329_4738629_-\|thioredoxin-family-protein	gnl\|CDD\|239245	cd02947, TRX_family, TRX family; composed of two groups: Group I, which includes proteins that exclusively encode a TRX domain; and Group II, which are composed of fusion proteins of TRX and additional domains. Group I TRX is a small ancient protein that alter the redox state of target proteins via the reversible oxidation of an active site dithiol, present in a CXXC motif, partially exposed at the protein's surface. TRX reduces protein disulfide bonds, resulting in a disulfide bond at its active site. Oxidized TRX is converted to the active form by TRX reductase, using reducing equivalents derived from either NADPH or ferredoxins. By altering their redox state, TRX regulates the functions of at least 30 target proteins, some of which are enzymes and transcription factors. It also plays an important role in the defense against oxidative stress by directly reducing hydrogen peroxide and certain radicals, and by serving as a reductant for peroxiredoxins. At least two major types of functional TRXs have been reported in most organisms; in eukaryotes, they are located in the cytoplasm and the mitochondria. Higher plants contain more types (at least 20 TRX genes have been detected in the genome of Arabidopsis thaliana), two of which (types f amd m) are located in the same compartment, the chloroplast. Also included in the alignment are TRX-like domains which show sequence homology to TRX but do not contain the redox active CXXC motif. Group II proteins, in addition to either a redox active TRX or a TRX-like domain, also contain additional domains, which may or may not possess homology to known proteins.	3.82744e-18
NZ_CP031068.1\|WP_000601778.1\|4736395_4737421_-\|methionine-ABC-transporter-ATP-binding-protein	gnl\|CDD\|224058	COG1135, AbcC, ABC-type metal ion transport system, ATPase component [Inorganic ion transport and metabolism].	8.75714e-177
NZ_CP031068.1\|WP_065702912.1\|4744774_4745488_-\|CPBP-family-intramembrane-metalloprotease	gnl\|CDD\|376806	pfam02517, Abi, CAAX protease self-immunity. Members of this family are probably proteases (after a isoprenyl group is attached to the Cys residue in the C-terminal CAAX motif of a protein to attach it to the membrane, the AAX tripeptide being removed by one of the CAAX prenyl proteases). The family contains the CAAX prenyl protease. The proteins contain a highly conserved Glu-Glu motif at the amino end of the alignment. The alignment also contains two histidine residues that may be involved in zinc binding. While they are involved in membrane anchoring of proteins in eukaryotes, little is known about their function in prokaryotes. In some known bacteriocin loci, Abi genes have been found downstream of bacteriocin structural genes where they are probably involved in self-immunity. Investigation of the bacteriocin-like loci in the Gram positive bacteria locus from Lactobacillus sakei 23K confirmed that the bacteriocin-like genes (sak23Kalphabeta) exhibited antimicrobial activity when expressed in a heterologous host and that the associated Abi gene (sak23Ki) conferred immunity against the cognate bacteriocin. Interestingly, the immunity genes from three similar systems conferred a high degree of cross-immunity against each other's bacteriocins, suggesting the recognition of a common receptor. Site-directed mutagenesis demonstrated that the conserved motifs constituting the putative proteolytic active site of the Abi proteins are essential for the immunity function of Sak23Ki - thus a new concept in self-immunity.	4.69629e-17
NZ_CP031068.1\|WP_001002987.1\|4744180_4744429_-\|spore-coat-protein	gnl\|CDD\|377935	pfam07875, Coat_F, Coat F domain. The Coat F proteins, which contribute to the Bacillales spore coat. It occurs multiple times in the genomes it is found in.	9.62029e-15
NZ_CP031068.1\|WP_141403578.1\|4742984_4744019_-\|L-lactate-dehydrogenase	gnl\|CDD\|133427	cd05291, HicDH_like, L-2-hydroxyisocapronate dehydrogenases and some bacterial L-lactate dehydrogenases. L-2-hydroxyisocapronate dehydrogenase (HicDH) catalyzes the conversion of a variety of 2-oxo carboxylic acids with medium-sized aliphatic or aromatic side chains. This subfamily is composed of HicDHs and some bacterial L-lactate dehydrogenases (LDH). LDHs catalyze the last step of glycolysis in which pyruvate is converted to L-lactate. Bacterial LDHs can be non-allosteric or may be activated by an allosteric effector such as fructose-1,6-bisphosphate. Members of this subfamily with known structures such as the HicDH of Lactobacillus confusus, the non-allosteric LDH of Lactobacillus pentosus, and the allosteric LDH of Bacillus stearothermophilus, show that they exist as homotetramers. The HicDH-like subfamily is part of the NAD(P)-binding Rossmann fold superfamily, which includes a wide variety of protein families including the NAD(P)-binding domains of alcohol dehydrogenases, tyrosine-dependent oxidoreductases, glyceraldehyde-3-phosphate dehydrogenases, formate/glycerate dehydrogenases, siroheme synthases, 6-phosphogluconate dehydrogenases, aminoacid dehydrogenases, repressor rex, and NAD-binding potassium channel domains, among others.	0
NZ_CP031068.1\|WP_000218968.1\|4739787_4740153_-\|arsenate-reductase-family-protein	gnl\|CDD\|239334	cd03036, ArsC_like, Arsenate Reductase (ArsC) family, unknown subfamily; uncharacterized proteins containing a CXXC motif with similarity to thioredoxin (TRX)-fold arsenic reductases, ArsC. Proteins containing a redox active CXXC motif like TRX and glutaredoxin (GRX) function as protein disulfide oxidoreductases, altering the redox state of target proteins via the reversible oxidation of the active site dithiol. ArsC catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)], using reducing equivalents derived from glutathione via GRX, through a single catalytic cysteine.	6.13259e-61
NZ_CP031068.1\|WP_000416291.1\|4747366_4749151_-\|acyl-CoA-dehydrogenase	gnl\|CDD\|173850	cd01161, VLCAD, Very long chain acyl-CoA dehydrogenase. VLCAD is an acyl-CoA dehydrogenase (ACAD), which is found in the mitochondria of eukaryotes and in some bacteria. It catalyzes the alpha,beta dehydrogenation of the corresponding trans-enoyl-CoA by FAD, which becomes reduced. The reduced form of ACAD is reoxidized in the oxidative half-reaction by electron-transferring flavoprotein (ETF), from which the electrons are transferred to the mitochondrial respiratory chain coupled with ATP synthesis. VLCAD acts as a homodimer.	0
NZ_CP031068.1\|WP_000722399.1\|4734026_4734833_-\|MetQ/NlpA-family-ABC-transporter-substrate-binding-protein	gnl\|CDD\|224381	COG1464, NlpA, ABC-type metal ion transport system, periplasmic component/surface antigen [Inorganic ion transport and metabolism].	1.42242e-111
NZ_CP031068.1\|WP_000026896.1\|4739362_4739746_-\|glycine-cleavage-system-protein-GcvH	gnl\|CDD\|234918	PRK01202, PRK01202, glycine cleavage system protein GcvH.	6.96592e-70
NZ_CP031068.1\|WP_000713771.1\|4741235_4741883_+\|phosphatase-PAP2-family-protein	gnl\|CDD\|239481	cd03386, PAP2_Aur1_like, PAP2_like proteins, Aur1_like subfamily. Yeast Aur1p or Ipc1p is necessary for the addition of inositol phosphate to ceramide, an essential step in yeast sphingolipid synthesis, and is the target of several antifungal compounds such as aureobasidin.	1.59372e-15
NZ_CP031068.1\|WP_000826143.1\|4734902_4735715_-\|MetQ/NlpA-family-ABC-transporter-substrate-binding-protein	gnl\|CDD\|224381	COG1464, NlpA, ABC-type metal ion transport system, periplasmic component/surface antigen [Inorganic ion transport and metabolism].	1.57398e-113
NZ_CP031068.1\|WP_000666172.1\|4741948_4742962_-\|PTS-sugar-transporter-subunit-IIC	gnl\|CDD\|379113	pfam13303, PTS_EIIC_2, Phosphotransferase system, EIIC. The bacterial phosphoenolpyruvate: sugar phosphotransferase system (PTS) is a multi-protein system involved in the regulation of a variety of metabolic and transcriptional processes. The sugar-specific permease of the PTS consists of three domains (IIA, IIB and IIC). The IIC domain catalyzes the transfer of a phosphoryl group from IIB to the sugar substrate.	1.57356e-86
NZ_CP031068.1\|WP_000359401.1\|4735737_4736403_-\|ABC-transporter-permease	gnl\|CDD\|224922	COG2011, AbcD, ABC-type metal ion transport system, permease component [Inorganic ion transport and metabolism].	8.57648e-99
NZ_CP031068.1\|WP_098294023.1\|4745952_4746501_-\|TetR/AcrR-family-transcriptional-regulator	gnl\|CDD\|379115	pfam13305, WHG, WHG domain. This presumed domain is around 80 amino acids in length. It is found to the C-terminus of a DNA-binding helix-turn-helix domain. This domain may be involved in binding to an as yet unknown ligand that allows a transcriptional regulation response to that molecule. The domain is named WHG after three conserved residues near the C-terminus of the domain.	5.15757e-25
NZ_CP031068.1\|WP_000929160.1\|4733002_4733788_-\|Fe-S-cluster-assembly-ATPase-SufC	gnl\|CDD\|223473	COG0396, sufC, Cysteine desulfurase activator ATPase [Posttranslational modification, protein turnover, chaperones].	8.75164e-150

>NZ_CP031068.1|WP_000218968.1|4739787_4740153_-|arsenate-reductase-family-protein
MTVTFYSYPKCGTCQKAKKWFEANDVAYEMIHIVENPPSKEDLRNLHEKSELPLKKFFNTSGMRYRELGLKDKLKDASEDEMYELLASDGMLIKRPIVTDGTNVTLGFNEEQFESVWKKYQ
>NZ_CP031068.1|WP_000026896.1|4739362_4739746_-|glycine-cleavage-system-protein-GcvH
MSIPNNLRYSEEHEWVKTEGNEVVIGITHFAQGELGDIVFVELPEVGATIEADEPFGSVESVKTVSELYAPVSGKVVAVNEELSDQPELVNESPYEGAWMVKVELSDASQVEKLLTAEKYAEMTNQD
>NZ_CP031068.1|WP_000640870.1|4738641_4738986_-|hypothetical-protein
MIYVEKVIIVEGTSDRRKIESIIREPVEIVCTNGTIGLSKMDELVDQFFDKEVYVLVDADDAGEKLRKQFRKEFPQAEHIYIDRSYREVATAPSTHLANVLWGADIDVYTEYLR
>NZ_CP031068.1|WP_000568176.1|4738329_4738629_-|thioredoxin-family-protein
MIDWTGAEATALIENEEKTVLYVYTPMCGTCQLAKKMLTVVEMTIEGLKIGMLDLNYAPYLAKEYGIESVPCLLVFENGTLMKKIYAFHSVEYLYTELK
>NZ_CP031068.1|WP_001242139.1|4737832_4738177_-|hypothetical-protein
MRLYSLLQSFVNYANGQYHLEPLLRQEEKTVNFRFGDESCSLQISRDYIELVHEERGTEQIICLDEKDIEQLVNGNVRLQMLMNDGRVQYSGTYRTMLLVESMFHICKPMSVGA
>NZ_CP031068.1|WP_000601778.1|4736395_4737421_-|methionine-ABC-transporter-ATP-binding-protein
MILLENVKKIYKAKSGDVTAVDNANLKIEKGEIFGVIGYSGAGKSSLIRLFNQLEKPTSGQITIANRVISAITGSELRKARQEIGMIFQHFNLLWSRTVRENIEFPLEIAGVDKAKRRKRVDELIHLVGLEGRGDAYPSQLSGGQKQRVGIARALANNPQVLLCDEATSALDPETTDQILDLLLDINKRLGLTIVLITHEMHVIRKICNRVAVMERGKIVETGPVLDVFRNPKQDITKRFVQQLTDSEDTNETIENLIEKYPDGKVIRLQFIGEAVERPVLQRLMQRSDIEVSILQGNIAQTNNGSYGSLVVHLNGEETAIQQAIEGIHQDQVELEVIAHG
>NZ_CP031068.1|WP_000359401.1|4735737_4736403_-|ABC-transporter-permease
MDKLLANVDWNQMLEATGETLYMTAIAALATFVLGLILGLLLFMTAKDNLWENKSINTVIGAFVNIFRSIPFIILIILLIPFTKILLGTILGASAALPALIIGAAPFYARMVEIALREIDKGVIEASKAMGAKTSTIILKVLIPESLPALVSGITVTTIALVGYTAMAGVVGAGGLGTLAYLEGFQRGNNDVTIVATICVLLVVFLIQWIGDSLTTRIDKR
>NZ_CP031068.1|WP_000826143.1|4734902_4735715_-|MetQ/NlpA-family-ABC-transporter-substrate-binding-protein
MKRLLLTALISTSIFGLAACGGKDKDEKKLVVGASNVPHAEILEKAKPLLEKKGIELEVKKFQDYVLPNKSLADKELDANYFQHIPYLEKEIKDKKYDFEIAGKIHIEPIGVYSQKYKSLKELPDGATIIMSNSVTDHGRGLAILQKEGILKIKDGVDPVSATPKDIADNPKHLKFKTDIEPGLLPQVYNNKEGDAVLINSNYAIDAKLNPEKDAIAIEGDDSPYANVLVVRKGDKDKKAIKELVEVLHSKEIKDFITKEYKGAVVPVKE
>NZ_CP031068.1|WP_000722399.1|4734026_4734833_-|MetQ/NlpA-family-ABC-transporter-substrate-binding-protein
MKKILLSVVTALSVFTLAACGGKEENKLVVGASNVPHAVILEKAQPILEKKGIKLEIKKFQDYVLPNKALADKEIDANYFQHIPYLDKEIQEKGYKIVNAGKIHLEPMGIYSKKYKSLKDLPDGGTVIMSNNVAERGRMLALLQKGGVIKLKDGVDVVKATVKDVVENPKNLKFKTDVEPGLSPKLYENNEGDALFINSNYAIDAKLNPTKDAIAIEGADSPYANIIAVRKGDEKKKEIKELVEVLHSKEIQDFINKEYKGAVLPVSE
>NZ_CP031068.1|WP_000929160.1|4733002_4733788_-|Fe-S-cluster-assembly-ATPase-SufC
MAGSTLTVKDLHVSIDGKEILKGVNLEVKGGEIHAIMGPNGTGKSTLSSAIMGHPKYEVTEGSIIIDGEDVLEMEVDERAQAGLFLAMQYPSEISGVTNADFLRSAINARREEGDEISLMKFIRTLDKNMEFLEMDPEMAQRYLNEGFSGGEKKRNEILQLMMIEPKIAILDEIDSGLDIDALKVVSKGINEMRGEEFGCLMITHYQRLLNYITPDFVHVMMNGRIVKSGGPELAQRLEAEGYDWIKKELGIEDETTEQEA
>NZ_CP031068.1|WP_000826869.1|4740564_4741092_-|hypothetical-protein
MKRLSYFLFFILICLVGAGCAKDKAGEKLEYNGRALVIGVVGEKPKDTFRNVKFEEMKLEELEKKSKEVDGFLIMKDHFQEASTEQYKNVFSSLKKPVFFIGLQDKSYSIFITKGIEYNSARKDVNAMYTQGFANIGSGEGQQWAIGLSNGGNTEESIHNMYIVVFQTIADYLNR
>NZ_CP031068.1|WP_000713771.1|4741235_4741883_+|phosphatase-PAP2-family-protein
MKKFKLSSFLPLSYILLLVLVSPLYDVLNKSTVHAVDVTTVVDDWIPFVKAFIIPYLLWFPYLYGALIYYCFADRKQYYVTLSSVIFGKLACFSIYYFWQTTVPRPTVVGTDVFSELVRYIYSIDQPVNCFPSIHVLTTFVIMLAAFKRREQHAFEYYILTFFGTLIILSTLFTKQHAFVDAISGMTLASILYFGVQLLLAKEPIRVPVKQNHRM
>NZ_CP031068.1|WP_000666172.1|4741948_4742962_-|PTS-sugar-transporter-subunit-IIC
MKEYIMSRVFKASAGIAQGIFVSLGIGLLIENIGRIVDIPLLITIGVVAKSLMAPAIGAGIAFMLGANGLVIFSAMVAGAIGAGSISITEAGLIIKTGEPIGALLTATLAVYIGKRLSGKTALDMMLVPFAAILGSGLVGIWLSHNITPVLNAVGAFIKDSSAGSPFIASIVIAVVWGLLLISPASSAALAIALSLDGVAGGAALAGCVAQFIGFSVISAKENNLGGILAQALCTPKVQLPNITKNPMILVPTVVASAIVGPVSALIFQLEAGKEIAGLGLSSLIAPINLISSQGWEVLPAMVITYIVIPVAVSYILYIALKKAGRIHSGDMTVPQS
>NZ_CP031068.1|WP_141403578.1|4742984_4744019_-|L-lactate-dehydrogenase
MVSIVITSIIRSVKSKRNRLYDQLGEIAMKRHTRKIAIIGTGLVGSSCAYSIVNQGICEELLLIDINHERAVGEAMDLSHCINFTNTRTKVYAGSYEDCKDMDIVIITAGPAPKPGQSRLDTLGASAKIMESVVGGVMESGFDGIFLLASNPVDIITYEVWKLSGLPRNRVIGTGTSLDSSRLRTILSEMLHVDPRSIHGYSLGEHGDSQMVAWSHVTVGGKPILQILEEQKERFGKIDLDEIVEKTAKAGWEIYKRKGTTYYGIGNSLAYIASSIFNDDHRVIAVSAILDGEYGEYDICTGVPAIITRDGIREVVELNLTEDEESRFAKSNDILRDYMKTIGY
>NZ_CP031068.1|WP_001002987.1|4744180_4744429_-|spore-coat-protein
MNEKDMVNDYLAGLNASLTSYANYIAQSDNEQLHQTLIQIRNQDEMRQRNMYEYAKQKSYYKPAAPANPMIVQQLKSQLSAE
>NZ_CP031068.1|WP_001180551.1|4744442_4744628_-|hypothetical-protein
MQNLTELEVENLRHLIGGHATIINKLEQYAETCTDPQLKQMLQKDAQDARNTKQQLMTFLG
>NZ_CP031068.1|WP_065702912.1|4744774_4745488_-|CPBP-family-intramembrane-metalloprotease
MSKTVLEAYSVERKIENITYKNLFVMIAMIIGISLLGGLFLGLAFEIYGEEALSTKLEGYYLLLFDAIVVAIVLLAYKPVFHFIKSIWDVSVLKSGKTYLYLLIGFIIIAVSQYLMLHVFSFESAAEQKEQLGSLRLQNSIQSIIYVLSVAIITPVKEEILFRGILYRFLEKRYNFLVSIIISSFIFGILHGGLLITATIMGMVFAMLYKKTQSIIPSIILHIVWNLLVSISMIVSL
>NZ_CP031068.1|WP_098294023.1|4745952_4746501_-|TetR/AcrR-family-transcriptional-regulator
MSPRIGLTLQKIVETAAEIADTNGIQEVTLASLAQRLGIRSPSLYNHVKGLQDVRKNLGIYGIKQLHNKLEEAAEDKRMDEAIHALGEVYVAFVRKHPGLYEATFLRDEEVRKAGDGIVKLCLQVLQQYGLEGENALHATRGFRSICHGFASIEQQGGFGLPLDLDISLHVLLETFIKGLRE
>NZ_CP031068.1|WP_149216684.1|4746504_4747227_-|MBL-fold-metallo-hydrolase
MRMIHEKTLYQLSFLPRAFPVNCYFVEEEDGLTLIDAALPYSAKGILQAAEKIGKPITKIVLTHAHDDHIGALDALKEVLPHVPVYISKRDAKLLEGDTTLQKDEPNIPIKGGVPKKVKTVPDVLLEDGDRVGSLLAIMTPGHTPGSMSFLDVRNKALIVGDAFQTRGGMAVSGQMKFWFPFPAMATWSKEISLQSAEKLSEYEPSLLAAGHGEMIKNPGTVIELAIKEAKRNIESKKEE
>NZ_CP031068.1|WP_000416291.1|4747366_4749151_-|acyl-CoA-dehydrogenase
MEKTVGNAVKGGSFLVDEITIDQVFTPEDFSSEHKMIAKTTEDFIVNEVLPELEYLEQHEFDRSVRLLKEAGELGLLGADVPEEYGGIGLDKVSSALIAEKFSRAGGFAITHGAHVGIGSLPIVLFGNEEQKKKYLPLLATGEKLAAYALTEPGSGSDALGAKTTARLNAEGTHYVLNGEKQWITNSAFADVFVVYAKIDGEHFSAFIVEKDYAGVSTSPEEKKMGIKCSSTRTLILEDALVPKENLLGEIGKGHIIAFNILNIGRYKLGVGTVGSAKRAVEISAQYANQRQQFKQPIARFPLIQEKLANMAAKTYAAESSVYRTVGLFESRMSTLSEEEVKDGKAVAASIAEYAIECSLNKVFGSEVLDYTVDEGVQIHGGYGFMAEYEIERMYRDSRINRIFEGTNEINRLIVPGTFLRKAMKGELPLLQKAQKLQEELMMMMPEEVGDEPLALQKYLVTNAKKIGLMVAGLAAQKYGKALDKEQEILVNIADIVSNLYAMESAVLRTEKAIKTTGLEKNKQKVLYTEVFCQEAFNEIEADAKETLIAVENGDMLRMMLSSLRKLTRHTPLNVIPKKREIAAKILEDERYTV

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Crispr_ID: NZ_CP031068_4

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP031068_4

5009063-5009196

Orphan

Consensus_repeat	Method
GTTGATTTCTCTTCTTTTTGAGA	CRISPRCasFinder

2 spacers

The CRISPR arrays of NZ_CP031068_4

>merge|NZ_CP031068|4|5009063-5009196|CRISPRCasFinder
GTTGATTTTTCTTCTTTCTTCGGTTCTTCTTTTTTGTCACTAGTTGAACCGCTTGTTGATTTCTCTTCTTTTTGAGACTTGTCTTCTGTTTTACTTTCAGGTTTCGTGCTCGTTGACTTCTCTTCTTTTTGAGA

>NZ_CP031068|4|3|5009063-5009196|CRISPRCasFinder
GTTGATTTTTCTTCTTTCTTCGG	TTCTTCTTTTTTGTCACTAGTTGAACCGCTT
GTTGATTTCTCTTCTTTTTGAGA	CTTGTCTTCTGTTTTACTTTCAGGTTTCGTGCTC
GTTGACTTCTCTTCTTTTTGAGA

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP031068.1\|WP_000008903.1\|5008740_5008875_-\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031068.1\|WP_016111010.1\|5004080_5004824_-\|chain-length-regulator-(capsular-polysaccharide-biosynthesis)	unknown	unknown	gnl\|CDD\|226453
NZ_CP031068.1\|WP_098005322.1\|5002509_5003277_-\|tyrosine-protein-phosphatase	unknown	unknown	gnl\|CDD\|226870
NZ_CP031068.1\|WP_000457978.1\|5006998_5008000_-\|rod-shape-determining-protein	unknown	unknown	gnl\|CDD\|237563
NZ_CP031068.1\|WP_097999379.1\|5001385_5002267_-\|UTP--glucose-1-phosphate-uridylyltransferase-GalU	unknown	unknown	gnl\|CDD\|224131
NZ_CP031068.1\|WP_098290722.1\|5018205_5019708_-\|NADH-quinone-oxidoreductase-subunit-M	unknown	unknown	gnl\|CDD\|235622
NZ_CP031068.1\|WP_149216713.1\|5016683_5018204_-\|NADH-quinone-oxidoreductase-subunit-NuoN	unknown	unknown	gnl\|CDD\|235603
NZ_CP031068.1\|WP_000411274.1\|5014144_5015449_-\|UDP-N-acetylglucosamine-1-carboxyvinyltransferase	unknown	unknown	gnl\|CDD\|236486
NZ_CP031068.1\|WP_000057889.1\|5016244_5016481_-\|DUF1146-domain-containing-protein	unknown	unknown	gnl\|CDD\|227173
NZ_CP031068.1\|WP_000672659.1\|5012918_5013938_-\|stage-II-sporulation-protein-D	unknown	unknown	gnl\|CDD\|274332
NZ_CP031068.1\|WP_000931959.1\|5006136_5006571_-\|3-hydroxyacyl-ACP-dehydratase-FabZ	unknown	unknown	gnl\|CDD\|234568
NZ_CP031068.1\|WP_002005985.1\|5015488_5016199_-\|YwmB-family-TATA-box-binding-protein	unknown	unknown	gnl\|CDD\|370056
NZ_CP031068.1\|WP_098290720.1\|5010788_5011631_-\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|224054
NZ_CP031068.1\|WP_016111011.1\|5005099_5005777_-\|CpsD/CapB-family-tyrosine-protein-kinase	unknown	unknown	gnl\|CDD\|349772
NZ_CP031068.1\|WP_016111009.1\|5003389_5004091_-\|CpsD/CapB-family-tyrosine-protein-kinase	unknown	unknown	gnl\|CDD\|349772
NZ_CP031068.1\|WP_084063842.1\|5011811_5012819_-\|ATP-binding-cassette-domain-containing-protein	unknown	unknown	gnl\|CDD\|224054
NZ_CP031068.1\|WP_000568224.1\|5019704_5021567_-\|NADH-quinone-oxidoreductase-subunit-L	unknown	unknown	gnl\|CDD\|235836
NZ_CP031068.1\|WP_000144443.1\|5010084_5010789_-\|ABC-transporter-permease	unknown	unknown	gnl\|CDD\|378925
NZ_CP031068.1\|WP_000544012.1\|5008160_5008433_-\|sporulation-transcriptional-regulator-SpoIIID	unknown	unknown	gnl\|CDD\|378813
NZ_CP031068.1\|WP_017656716.1\|5000687_5001368_-\|sugar-transferase	unknown	unknown	gnl\|CDD\|376775

Protein	Function_ID	Function_description	E-value
NZ_CP031068.1\|WP_016111010.1\|5004080_5004824_-\|chain-length-regulator-(capsular-polysaccharide-biosynthesis)	gnl\|CDD\|226453	COG3944, COG3944, Capsular polysaccharide biosynthesis protein [Cell envelope biogenesis, outer membrane].	6.93137e-85
NZ_CP031068.1\|WP_098005322.1\|5002509_5003277_-\|tyrosine-protein-phosphatase	gnl\|CDD\|226870	COG4464, CapC, Capsular polysaccharide biosynthesis protein [Carbohydrate transport and metabolism / Cell envelope biogenesis, outer membrane].	2.05823e-106
NZ_CP031068.1\|WP_000544012.1\|5008160_5008433_-\|sporulation-transcriptional-regulator-SpoIIID	gnl\|CDD\|378813	pfam12116, SpoIIID, Stage III sporulation protein D. This stage III sporulation protein is a small DNA-binding family that is essential for gene expression of the mother-cell compartment during sporulation. The domain is found in bacteria and viruses, and is about 40 amino acids in length. It has a conserved RGG sequence motif.	1.17272e-38
NZ_CP031068.1\|WP_097999379.1\|5001385_5002267_-\|UTP--glucose-1-phosphate-uridylyltransferase-GalU	gnl\|CDD\|224131	COG1210, GalU, UDP-glucose pyrophosphorylase [Cell envelope biogenesis, outer membrane].	0
NZ_CP031068.1\|WP_098290722.1\|5018205_5019708_-\|NADH-quinone-oxidoreductase-subunit-M	gnl\|CDD\|235622	PRK05846, PRK05846, NADH:ubiquinone oxidoreductase subunit M; Reviewed.	1.8014e-177
NZ_CP031068.1\|WP_000457978.1\|5006998_5008000_-\|rod-shape-determining-protein	gnl\|CDD\|237563	PRK13928, PRK13928, rod shape-determining protein Mbl; Provisional.	0
NZ_CP031068.1\|WP_000411274.1\|5014144_5015449_-\|UDP-N-acetylglucosamine-1-carboxyvinyltransferase	gnl\|CDD\|236486	PRK09369, PRK09369, UDP-N-acetylglucosamine 1-carboxyvinyltransferase; Validated.	0
NZ_CP031068.1\|WP_000057889.1\|5016244_5016481_-\|DUF1146-domain-containing-protein	gnl\|CDD\|227173	COG4836, COG4836, Predicted membrane protein [Function unknown].	1.23409e-23
NZ_CP031068.1\|WP_000672659.1\|5012918_5013938_-\|stage-II-sporulation-protein-D	gnl\|CDD\|274332	TIGR02870, Stage_II_sporulation_protein_D, stage II sporulation protein D. Stage II sporulation protein D (SpoIID) is a protein of the endospore formation program in a number of lineages in the Firmicutes (low-GC Gram-positive bacteria). It is expressed in the mother cell compartment, under control of Sigma-E. SpoIID, along with SpoIIM and SpoIIP, is one of three major proteins involved in engulfment of the forespore by the mother cell. [Cellular processes, Sporulation and germination].	4.89572e-134
NZ_CP031068.1\|WP_000931959.1\|5006136_5006571_-\|3-hydroxyacyl-ACP-dehydratase-FabZ	gnl\|CDD\|234568	PRK00006, fabZ, 3-hydroxyacyl-ACP dehydratase FabZ.	5.26499e-95
NZ_CP031068.1\|WP_002005985.1\|5015488_5016199_-\|YwmB-family-TATA-box-binding-protein	gnl\|CDD\|370056	pfam08680, DUF1779, TATA-box binding. TATA-box_bdg is a family of bacterial proteins. YwmB from Bacillus subtilis contains a circularly permuted TATA-box binding protein-like fold. Jian-Xiang Liu, Qi Xie, Jun Lin. Protein Structural Data Mining and Evolutionary Bioinformatic Analysis on Domains of TATA-box Binding Protein-like Fold. Life Science Journal 2014; 11(2): 298-302 (not yet in PubMed 27-02-2014).	5.16446e-34
NZ_CP031068.1\|WP_149216713.1\|5016683_5018204_-\|NADH-quinone-oxidoreductase-subunit-NuoN	gnl\|CDD\|235603	PRK05777, PRK05777, NADH-quinone oxidoreductase subunit NuoN.	3.67718e-150
NZ_CP031068.1\|WP_016111011.1\|5005099_5005777_-\|CpsD/CapB-family-tyrosine-protein-kinase	gnl\|CDD\|349772	cd05387, BY-kinase, bacterial tyrosine-kinase. Bacterial tyrosine (BY)-kinases catalyze the autophosphorylation on a C-terminal tyrosine cluster and also phosphorylate endogenous protein substrates by using ATP as phosphoryl donor. Besides their capacity to function as tyrosine kinase, most of these proteins are also involved in the production and transport of exopolysaccharides. BY-kinases are involved in a number of physiological processes ranging from stress resistance to pathogenicity.	1.54553e-77
NZ_CP031068.1\|WP_016111009.1\|5003389_5004091_-\|CpsD/CapB-family-tyrosine-protein-kinase	gnl\|CDD\|349772	cd05387, BY-kinase, bacterial tyrosine-kinase. Bacterial tyrosine (BY)-kinases catalyze the autophosphorylation on a C-terminal tyrosine cluster and also phosphorylate endogenous protein substrates by using ATP as phosphoryl donor. Besides their capacity to function as tyrosine kinase, most of these proteins are also involved in the production and transport of exopolysaccharides. BY-kinases are involved in a number of physiological processes ranging from stress resistance to pathogenicity.	9.52145e-83
NZ_CP031068.1\|WP_084063842.1\|5011811_5012819_-\|ATP-binding-cassette-domain-containing-protein	gnl\|CDD\|224054	COG1131, CcmA, ABC-type multidrug transport system, ATPase component [Defense mechanisms].	1.8738e-31
NZ_CP031068.1\|WP_000568224.1\|5019704_5021567_-\|NADH-quinone-oxidoreductase-subunit-L	gnl\|CDD\|235836	PRK06590, PRK06590, NADH:ubiquinone oxidoreductase subunit L; Reviewed.	0
NZ_CP031068.1\|WP_000144443.1\|5010084_5010789_-\|ABC-transporter-permease	gnl\|CDD\|378925	pfam12698, ABC2_membrane_3, ABC-2 family transporter protein. This family is related to the ABC-2 membrane transporter family pfam01061.	0.00114363
NZ_CP031068.1\|WP_098290720.1\|5010788_5011631_-\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|224054	COG1131, CcmA, ABC-type multidrug transport system, ATPase component [Defense mechanisms].	1.41746e-90
NZ_CP031068.1\|WP_017656716.1\|5000687_5001368_-\|sugar-transferase	gnl\|CDD\|376775	pfam02397, Bac_transf, Bacterial sugar transferase. This Pfam family represents a conserved region from a number of different bacterial sugar transferases, involved in diverse biosynthesis pathways.	1.90266e-98

>NZ_CP031068.1|WP_000008903.1|5008740_5008875_-|hypothetical-protein
MSFLFVLSTAIILLFVLFTLYYYIAGKKEVPHHQLLEEELEREE
>NZ_CP031068.1|WP_000544012.1|5008160_5008433_-|sporulation-transcriptional-regulator-SpoIIID
MHDYIKERTIKIGKYIVETRKTVRVIAKEFGVSKSTVHKDLTERLPEINPELANEVKEILDYHKSIRHLRGGEATKQKYRKEDVEKPVRQ
>NZ_CP031068.1|WP_000457978.1|5006998_5008000_-|rod-shape-determining-protein
MFARDIGIDLGTANVLIHVKGKGIVLNEPSVVAIDRNSGKVLAVGEEARSMVGRTPGNIVAIRPLKDGVIADFEITEAMLKYFINKLDVKSFFSKPRILICCPTNITSVEQKAIREAAERSGGKTVFLEEEPKVAAVGAGMEIFQPSGNMVVDIGGGTTDIAVLSMGDIVTSSSIKMAGDKFDMEILNYIKRKYKLLIGERTSEDIKIKVGTVFPGARSEELEIRGRDMVTGLPRTITVCSEEITEALKENAAVIVQAAKGVLERTPPELSADIIDRGVILTGGGALLHGIDMLLAEELKVPVLIAENPMHCVAVGTGIMLENIDRLPKRALR
>NZ_CP031068.1|WP_000931959.1|5006136_5006571_-|3-hydroxyacyl-ACP-dehydratase-FabZ
MLNIEQIKEIIPHRYPFLLVDKILEVDEGKRAVGIKNVSANEEFFNGHFPDYAVMPGVLIVEALAQVGAVAVLKKEENRGRLAFFAGIDNCRFKKQVRPGDQLRLEVEMTRVRGPIGKGKAIATVDGEVACEAEITFAIGDKKE
>NZ_CP031068.1|WP_016111011.1|5005099_5005777_-|CpsD/CapB-family-tyrosine-protein-kinase
MFGRKKRKPLRQLITHKEPKSRIAEQYRNIRTNIEFTSVDNHIRSIIVTSVDPGDGKTTTIANLAVVFGQQGKKVLLIGADLRKPTLQNLFAAYSPNGLTNLLSGQTSFVQCIQKTDIENVYIMSAGPIPPNPAELLGYRKMDEVLLEAYKMFDIVLIDTPPVMAVTDAQILANKCDGVVLVARSEKTEKDKLIKVKQILDKASGKLLGVILNDKREEQESYGYY
>NZ_CP031068.1|WP_016111010.1|5004080_5004824_-|chain-length-regulator-(capsular-polysaccharide-biosynthesis)
MEETISLKELFHILKKRLAMILVIAFGAAIVSAIISFFFMTPIYQSSTQILVNQKKQEGAAIQYNEVQTNIQLTNTYKVIIKSPVILDQVKEKLGLNITVQELNNKIEVANEKDSQVVAVTAQDKDPKLARDIANTTAEVFKGEVAKIMSVDNVTVLSKAEVAENLSPIKPRPMLNVAIAFVIGLMASIGLAFLLEYLDNTVKKEEDVESLLGLPVLGIVARMDEETLNVKSHAPSSRKVRGQTIGS
>NZ_CP031068.1|WP_016111009.1|5003389_5004091_-|CpsD/CapB-family-tyrosine-protein-kinase
MALNLFKKKKNHRQRRQLIAHQQPKSPISEQYRNIRTNIEFAAVDLNLHSLMVTSANPSEGKTTTTANMAVVFAQQGKKVLLIDADMRKPAMHQMFQVDNIFGLTNVLTYSERLEKCVQTTSIDNLSFLACGPIPPNPAELLGSKSMKELLGQAYSMYDLVIFDMPPILAVTDAQIMANVCDASILVVRSESTEKETAVKAKGLLESAKGKLLGVVLNDREREEGLYYYYGAN
>NZ_CP031068.1|WP_098005322.1|5002509_5003277_-|tyrosine-protein-phosphatase
MIDLHCHILPGIDDGAQTVTDSLAMAQQAVSEGIHTIVATPHHQNGKYINERTSIIHGVKQLNDELQQHNIPLTILPGQEVRLYGDLLEDYETGKIVTLNETNKYIFIEFPSNHVPRYAEQLLYELRVKGMIPIIVHPERNAELIERPDKLYNLVNKGTLTQVTAGSLLGKFGKKIKKFSLQLVEHNLTHMVASDAHNTTSRGFHLAESYELIEKEFGMSVMSDLKENPYLLISGKAIYKEDPEQIRRKKLFGIF
>NZ_CP031068.1|WP_097999379.1|5001385_5002267_-|UTP--glucose-1-phosphate-uridylyltransferase-GalU
MKKVRKAIIPAAGLGTRFLPATKAMPKEMLPIVDKPTIQYIIEEAIESGIEDIIIVTGKGKRAIEDHFDHSFELEQNLLEKGKYEMLEKVQASSKINIHYIRQKEPKGLGHAVWCARKFIGNEPFAVLLGDDIVQAETPCLRQLMDQYEGTQSSVIGVQTVPENETHRYGIIDPVEQNDRRYQVRQFVEKPEQGTAPSNLAIMGRYVLTPEIFMFLENQETGAGGEIQLTDAIQRLNEIQRVFAYDFEGTRYDVGEKFGFIKTTIEMALQHESLREDLISYMNELVKKENVHI
>NZ_CP031068.1|WP_017656716.1|5000687_5001368_-|sugar-transferase
MALTKVSSGVVKEQRMLVAEEINQSVFYLFMKRVIDILGSLCGLIFLFPVFLVIALLIKLEDPKGSILFKQVRVGKNEKEFGMYKFRSMVTDAEERLKDLLEHNEVSGAMFKMKEDPRVTKIGKFIRKTSIDELPQLLNVLKGEMSLVGPRPPLPREVKEYTLYDKQRLLVTPGCTGLWQVTERNSVGFAEMVELDLTYIKKRNIRFDCKLILKTVLVLVGSKDAY
>NZ_CP031068.1|WP_000144443.1|5010084_5010789_-|ABC-transporter-permease
MTFSMRRVSAIFRKEVQDFKTNSQVLLMAFLPIILSFLFSRFGAGKEMLGITTITAFLFVAGFVQSMVIAEEKEKHTLRVLMLSPASSVEVLLGKSILTACLTLGICIVNLFILDQFSGNLLLLGFIFLCGTILFIVIGTMIGLLAASVPQTSIIGMPILMTMYLAVQFEPMVENKVIKTMIGYLPTSHIVKALNSLVGGTGFGSISGHVLNVAVWLIISLFVCLIVYKKKQLD
>NZ_CP031068.1|WP_098290720.1|5010788_5011631_-|ABC-transporter-ATP-binding-protein
MTLAIEMKDVMKSFNEKTALRNVNIEVKQGEIFGFLGPSGSGKTTTVKILTSQLLHSVGTVRVLGKDITGPSSIDYKRIGILTDNSGLYERLSIYDNLLLFCDLYDCKKERIDEVLAQVNLLDDKKTPVKKLSKGMKQRVTLARAILHKPDILFLDEPTSALDPVNVQNIHKILKDLNKEGTTIFLTTHNMDEAETLCNRIAFLCGGEIVAIDTPENLRLQYAKDQIQVVLKDKKKETVQKDELGAKRISEWMNKGELLSIHSHEPTLGDIFIEVTGRGL
>NZ_CP031068.1|WP_084063842.1|5011811_5012819_-|ATP-binding-cassette-domain-containing-protein
MALLELKQLGKTNQLPEIELKVEKGQCIVLQCNNHTAKVLHRIIIGEEEASTGNVLFEGEAIGKKNYFRIGFCFLKDEAYDRLKVKEYFKFLLGLYESKVSIEEVVQYVGLLDKLNVKIEKLSFSEKRRLHIGRIMIHNPDLVILEEPEQNVDTESTIIIRKAIMKMKEQGKAIFITSSFLSDALSLTEDVYILNNDGVKKVEIEQEEVEEVGEEKVIQMIPQMKLERIPAKVNDKIILLDPMEIHFIETQNGVTHIHVREGDFVCALTLSELEARLTGFGFFRCHRSYLVNLQRVREVITWTRNSFSLILDDERKSSIPLSKGRMDELKGVIGL
>NZ_CP031068.1|WP_000672659.1|5012918_5013938_-|stage-II-sporulation-protein-D
MKFSKPLFITVALLIALVIIVPAALVIPFAKAKVGEETASKTPPAIESIPAPGKVDTAVQVAVYREKQKKVETLPVEEYVTGVVASEMNASFEIEALKAQALAARTFVVQRMLSGGKKNNADVTDTVKDQVYKGKDELKKQWGNNYENNLKKIEEAVSKTAGQVLTYDGKPISASFFSTSNGRTENAADYWGNDYPYLKSVDSPWDQASPKFTSEQTFTVADFQKRLGVKVLADGKVGNIKDLTEGKRVKDVAFQGKTLTGKEVREKLDLRSSDFTWKQQGDKIIVTTKGFGHGVGMSQYGANGMAAEGKKYTDIVAHYYKGVEIKTMNDYEGKLMVKK
>NZ_CP031068.1|WP_000411274.1|5014144_5015449_-|UDP-N-acetylglucosamine-1-carboxyvinyltransferase
MEKIIVRGGKRLNGTVRVEGAKNAVLPIIAAALLASDGKNVLSEVPVLSDVYTINEVLRHLNAEVVFENNQVTIDSSKELNIEAPFEYVRKMRASVQVMGPLLARNGRARIALPGGCAIGSRPIDQHLKGFEAMGAKVQVGNGFVEAYVEGELKGAKIYLDFPSVGATENIMSAATLAKGTTILENAAKEPEIVDLANFLNAMGAKVRGAGTGTIRIEGVDKLYGANHSIIPDRIEAGTFMVAAAITGGDILIENAVPEHLRSITAKMEEMGVKIIEENEGVRVIGPDKLKAVDIKTMPHPGFPTDMQSQMMALLLQADGTSMITETVFENRFMHVEEFRRMNADIKIEGRSVIMNGPNSLQGAEVAATDLRAAAALILAGLVSEGYTRVTELKHLDRGYVNFHKKLAALGATIERVNEKVEEVKEQEVSDLHA
>NZ_CP031068.1|WP_002005985.1|5015488_5016199_-|YwmB-family-TATA-box-binding-protein
MKLKLKAILIVALSVVLFLVGYREMKPISDEQKMESMIKALEKNDAKVEKWSWLARETKTISNIHTFQKLLNDVKEKANIQKWEVEQSPDGYKATSYKKLSSYEERVVVTWSKENTKENTFIIFEVSGAKWDPKYVQNMNKIFSEKPIIYTCVQGVLNDKIEGVLQNKTNQVLKDLSARAIEQVEERAFVSVSAYNKKWDDALSTNREKINVQIAIRSTDNKDTIVVGTPIITSEY
>NZ_CP031068.1|WP_000057889.1|5016244_5016481_-|DUF1146-domain-containing-protein
MAQLLGQQALIAIVSHLLFITITWWALQGIHIERLMKSGKVLQTRVLLILITIAIGTSVSNFFLDYLGYSKSLTYLVK
>NZ_CP031068.1|WP_149216713.1|5016683_5018204_-|NADH-quinone-oxidoreductase-subunit-NuoN
MDMNTLLSLSWHLMVPEFIILGAAILLSICDLFFKLNHRYVALGAIAAIVLAIVSLITLYSEPAGDILNGSFVLDGFSKGFKTLLLGASALILCTAMSDDKKNPIEDKGEYYYLFLMALLGAMFMASSVDFITLFVGLELLSLSSYILVGIRKKSRASNEAAMKYVISGGIGTAITLFGMSYLYGITGSTNIVDMQKVLTGELASGIQLLLALAFLLLLVGLSFKIATVPFHMWAPDVYEGAATPVTAFLGTISKIAGFLLIIRLFLMVFASVLIQGDMQSLYGHMSIYIAVLASITMIVGNVVALKQYNIKRLFAYSGIAHAGYLLVPLVALSPFTMDSMWFYMLAYMLMNIGAFAIIHGLILQNDEENITIFTGLYKRSPFAAIMMTIFILSLAGIPGTVGFIGKVNIFLGALHVEPAHYVLASIMMGTTVVSFVYYFRILQQMFFRTGETEERIRLPLNIKVVMSLCAISIVILGIMPMIGYNFFYEYFPLMKDFFFLGNVVQ
>NZ_CP031068.1|WP_098290722.1|5018205_5019708_-|NADH-quinone-oxidoreductase-subunit-M
MNDLLLTFFIFSPLLGILLLVLTPKKELRTVRALGLFGTALPFGIAIVLACTYASGKNLSLFDEKVKWIKFGDFTAVDKRWFSIYYELGIDGLSLVMMVLTALLAMLAAIAAFSIKRNLKAFYMLLLMLEIGMLGVFAAQNLMLFFIFFEITLPPMFLLIGKWGKLSSEKAAYSYLIYNGIGSAILLIVFSILFAKTGTTNIVELKEILRSVSAEGELVIPSSLQLGVFISIMIAFAIKLPVFPLHRWMVNVHIEAHPAVVMLHAGVLLKIGAYGIIRFGKGLFPEYFHDVATLIAILGVINLLYGAFLALIQTDFRKVLAYSSISHMGIVLMGLASLNAPGTQGALFQVVSHGLIAALLFFLLGVIEQRFGTSDITVLGGLAKSVPILSGFFLAGGMASLGLPGMSGFVSEFLAFLGLFQGEPVIAAVGVIGIILTAVYVLRATLQVTFGKKEWEAKSDIHGWEYVPVLLLIFCIIAIGVMPELLGDPLQNTLKTLGVK
>NZ_CP031068.1|WP_000568224.1|5019704_5021567_-|NADH-quinone-oxidoreductase-subunit-L
MIDYAWLIPLFPLVSFLLLIIFGKKIREGSSVLSIFFVFLSFILAVLVLIERFSEASVKHKWVWLRAGDVDISFGFEVTALGALMLFIVTLVSLLVHVYSKGYMKGDERLPTFYAYLGLFTFAMLGLVISTNLLQLYIFWELVGLGSFLLIGFYFFKEEAKAAAKKAFIMTRIGDVGLFIGMILIFWYAGSFEYDAIFRAIYTGDLPPYMITITAILIFIGAMGKSGQFPLHTWLPDAMEGPTPVSALIHAATMVAAGVYLVATMFPLFSASTVAMQTVAIVGAFTAIFAASIGLVQTDIKRVLAYSTVSQLGYMMLALGSAGYVAGIFHLTTHAFFKALLFLAAGSVIHAVHTQNINKMGGLQKKMKVTGTLFLIGTLAISGVPLLSGFFSKDEILAATWMNGNYVLFTLAVIAAFLTAFYMFRLYFLVFTGETKTKEEVHESPRVMTYPMIVLGVLAVVAGYINTPWFGTFLGDWLTKDVGFKVEEAHGPVWIMIVATLVSFAGIALAYFIYGKKSISRDWAGGTETSLYNLLKEKYYVDELYNMTVIPITKGIAHVLRLFEVYVVEGIAVLIGSVVKGTSNLGSKLQNGNVQVYGTVTAVSLAVLVIILLYTGGDLR

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
NZ_CP031068_1	1.4\|1954600\|21\|NZ_CP031068\|CRT	1954600-1954620	21	NZ_CP031068.1	1954429-1954449	1	0.952

1. spacer 1.4|1954600|21|NZ_CP031068|CRT matches to position: 1954429-1954449, mismatch: 1, identity: 0.952

ttgttcttcttgttgtttgcg	CRISPR spacer
ttgttcttcttgttgtttacg	Protospacer
******************.**

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP031068_1	1.4\|1954600\|21\|NZ_CP031068\|CRT	1954600-1954620	21	NZ_CP021331	Maritalea myrionectae strain HL2708#5 plasmid pHL2708X3, complete sequence	121222-121242	1	0.952
NZ_CP031068_1	1.4\|1954600\|21\|NZ_CP031068\|CRT	1954600-1954620	21	MK559459	Vibrio phage vB_VcaS_HC, complete genome	61426-61446	2	0.905
NZ_CP031068_4	4.1\|5009086\|31\|NZ_CP031068\|CRISPRCasFinder	5009086-5009116	31	JX238501	Bacillus phage phiAGATE, complete genome	124559-124589	8	0.742

1. spacer 1.4|1954600|21|NZ_CP031068|CRT matches to NZ_CP021331 (Maritalea myrionectae strain HL2708#5 plasmid pHL2708X3, complete sequence) position: , mismatch: 1, identity: 0.952

ttgttcttcttgttgtttgcg	CRISPR spacer
ttgttcatcttgttgtttgcg	Protospacer
****** **************

2. spacer 1.4|1954600|21|NZ_CP031068|CRT matches to MK559459 (Vibrio phage vB_VcaS_HC, complete genome) position: , mismatch: 2, identity: 0.905

ttgttcttcttgttgtttgcg	CRISPR spacer
acgttcttcttgttgtttgcg	Protospacer
 .*******************

3. spacer 4.1|5009086|31|NZ_CP031068|CRISPRCasFinder matches to JX238501 (Bacillus phage phiAGATE, complete genome) position: , mismatch: 8, identity: 0.742

ttcttcttttttgtcactagttgaaccgctt	CRISPR spacer
ctaaccttttttgccactagttaaaccgccc	Protospacer
.*  .********.********.******..

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

256668 : 264615

uncultured_virus(33.33%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000917311.1\|256668_256953_+	co-chaperone GroES	A0A221S3C8	uncultured_virus	1.2e-20	54.8
WP_001029993.1\|256991_258626_+	chaperonin GroEL	A0A219YK78	uncultured_virus	1.5e-157	58.0
WP_149215952.1\|259032_260571_+	glutamine-hydrolyzing GMP synthase	A0A1V0SH76	Hokovirus	8.8e-22	31.7
WP_000833096.1\|260955_262281_+	NCS2 family permease	A0A0R6PHV4	Moraxella_phage	4.1e-44	29.4
WP_000929880.1\|262424_263126_+	response regulator transcription factor	W8CYM9	Bacillus_phage	9.5e-40	36.0
WP_000719210.1\|263109_264615_+	aminopeptidase AmpS	W8CYF6	Bacillus_phage	7.8e-31	30.0

DBSCAN-SWA_2

302981 : 311357

Synechococcus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000625682.1\|302981_304289_+	adenylosuccinate lyase	A0A2H4UUU6	Bodo_saltans_virus	4.9e-21	25.3
WP_001170544.1\|304377_305097_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	G8EYA2	Synechococcus_phage	4.0e-49	44.3
WP_000278823.1\|305089_305344_+	phosphoribosylformylglycinamidine synthase subunit PurS	NA	NA	NA	NA
WP_000666787.1\|305340_306024_+	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
WP_000055564.1\|306007_308227_+	phosphoribosylformylglycinamidine synthase II	A6N228	Microbacterium_phage	2.0e-163	41.2
WP_000879032.1\|308211_309627_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	4.3e-55	35.2
WP_001262439.1\|309732_310773_+	phosphoribosylformylglycinamidine cyclo-ligase	A0A0E3F760	Synechococcus_phage	1.2e-67	45.9
WP_000088589.1\|310769_311357_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	9.1e-28	37.4

DBSCAN-SWA_3

1777235 : 1785350

Bacillus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000755498.1\|1777235_1778528_+	C40 family peptidase	A0A1V0DZX6	Clostridioides_phage	3.5e-11	31.5
WP_001194306.1\|1778627_1779392_-	alpha/beta hydrolase	NA	NA	NA	NA
WP_000453861.1\|1779632_1781393_+	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	2.4e-273	98.0
WP_000612414.1\|1781430_1782108_+	response regulator transcription factor	W8CYM9	Bacillus_phage	1.1e-122	99.1
WP_001231624.1\|1782104_1783178_+	HAMP domain-containing sensor histidine kinase	W8CYF6	Bacillus_phage	1.8e-186	97.8
WP_000818979.1\|1783467_1784187_+	3-ketoacyl-ACP reductase	W8CYX9	Bacillus_phage	5.9e-61	100.0
WP_001258541.1\|1784477_1785350_+	aminoglycoside 6-adenylyltransferase	E4ZFP8	Streptococcus_phage	1.6e-65	44.8

DBSCAN-SWA_4

2392434 : 2442725

Bacillus_phage(76.92%)

head,portal,holin,integrase,coat,bacteriocin,terminase,tail

attL 2385419:2385433|attR 2406746:2406760

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
2385419:2385433	attL	NA	NA	NA	NA
WP_001071364.1\|2392434_2392755_+\|bacteriocin	heterocycloanthracin/sonorensin family bacteriocin	NA	NA	NA	NA
WP_002063155.1\|2393244_2393730_+	hypothetical protein	NA	NA	NA	NA
WP_000736195.1\|2394039_2394741_+	DUF3962 domain-containing protein	NA	NA	NA	NA
WP_149216294.1\|2394779_2395889_-\|integrase	tyrosine-type recombinase/integrase	A0A0U3U8Y8	Bacillus_phage	3.0e-144	77.5
WP_001987382.1\|2396204_2396651_+	hypothetical protein	NA	NA	NA	NA
WP_149216295.1\|2396694_2396904_-	hypothetical protein	NA	NA	NA	NA
WP_149216296.1\|2397743_2398895_+	hypothetical protein	A0A1B0T6A5	Bacillus_phage	1.0e-51	33.6
WP_149216297.1\|2398932_2399079_+	complement C1q protein	NA	NA	NA	NA
WP_149216298.1\|2399401_2399755_-	helix-turn-helix domain-containing protein	A0A0U3SD25	Bacillus_phage	7.4e-49	86.2
WP_000854267.1\|2399980_2400172_+	helix-turn-helix transcriptional regulator	A0A0U3SLC2	Bacillus_phage	1.9e-22	85.2
WP_149216299.1\|2400228_2400504_+	helix-turn-helix domain-containing protein	A0A0U3ULL9	Bacillus_phage	2.9e-37	88.8
WP_149216300.1\|2400648_2400969_+	hypothetical protein	I3WU08	Bacillus_phage	6.1e-26	56.3
WP_098209496.1\|2400969_2401164_+	hypothetical protein	NA	NA	NA	NA
WP_071730504.1\|2401240_2401741_+	hypothetical protein	A0A2H4J6F5	uncultured_Caudovirales_phage	1.3e-19	37.7
WP_149216301.1\|2401741_2402686_+	hypothetical protein	S6C475	Thermus_phage	1.8e-81	49.7
WP_149216302.1\|2402701_2403493_+	recombination protein RecT	S6AVW6	Thermus_phage	1.8e-95	66.1
WP_149216303.1\|2403663_2404521_+	DnaD domain protein	A0A0S2MVA0	Bacillus_phage	1.6e-73	56.1
WP_149216751.1\|2404591_2405284_+	ATP-binding protein	A0A0S2GLK2	Bacillus_phage	1.5e-61	50.8
WP_149216304.1\|2405289_2405478_+	hypothetical protein	A0A0U3K3J8	Bacillus_phage	1.5e-13	52.5
WP_149216305.1\|2405458_2405941_+	IDEAL domain-containing protein	NA	NA	NA	NA
WP_149216752.1\|2406088_2406403_+	hypothetical protein	A0A0A7AQW3	Bacillus_phage	4.7e-23	46.6
WP_000049835.1\|2406958_2407375_+	DUF1064 domain-containing protein	A0A2P1JTY5	Anoxybacillus_phage	1.1e-32	54.1
2406746:2406760	attR	NA	NA	NA	NA
WP_149216306.1\|2407389_2408106_+	2-methylcitrate dehydratase	NA	NA	NA	NA
WP_149216307.1\|2408175_2409069_+	zinc-finger domain-containing protein	NA	NA	NA	NA
WP_081366294.1\|2409078_2409468_+	zinc-finger domain-containing protein	NA	NA	NA	NA
WP_088345966.1\|2410027_2410222_-	hypothetical protein	NA	NA	NA	NA
WP_149216308.1\|2411104_2411485_+	hypothetical protein	S5MUC4	Brevibacillus_phage	1.4e-08	49.1
WP_149216309.1\|2411559_2412027_-	epimerase	NA	NA	NA	NA
WP_149216310.1\|2412294_2412492_+	hypothetical protein	A0A1B1P864	Bacillus_phage	2.1e-13	61.5
WP_149216753.1\|2413319_2413520_+	hypothetical protein	NA	NA	NA	NA
WP_149216311.1\|2413671_2413962_-	DUF4183 domain-containing protein	NA	NA	NA	NA
WP_001110933.1\|2415065_2415281_-	spore germination protein	A0A2H4J3A3	uncultured_Caudovirales_phage	7.4e-28	88.7
WP_149216312.1\|2415641_2416019_+	ArpU family transcriptional regulator	A0A2H4J748	uncultured_Caudovirales_phage	4.3e-23	50.4
WP_098394576.1\|2417189_2417396_-	hypothetical protein	NA	NA	NA	NA
WP_149216313.1\|2417762_2417945_+	hypothetical protein	NA	NA	NA	NA
WP_149216314.1\|2418373_2418580_-	hypothetical protein	NA	NA	NA	NA
WP_149216315.1\|2419268_2420207_+\|terminase	terminase	A0A0S2MVB6	Bacillus_phage	2.4e-115	83.9
WP_149216316.1\|2420193_2421465_+\|terminase	PBSX family phage terminase large subunit	A0A1L2JY46	Aeribacillus_phage	1.7e-220	84.6
WP_149216317.1\|2421477_2422911_+\|portal	phage portal protein	A0A0S2MVB2	Bacillus_phage	2.4e-271	97.5
WP_149216754.1\|2422984_2423752_+\|head	phage head protein	A0A0S2MVF0	Bacillus_phage	3.2e-137	96.5
WP_149216318.1\|2423824_2424538_+	DUF4355 domain-containing protein	A0A0A7AQU8	Bacillus_phage	2.4e-70	69.5
WP_149216319.1\|2424647_2425493_+	hypothetical protein	A0A0A7AQF5	Bacillus_phage	1.6e-145	94.3
WP_149216320.1\|2425689_2425998_+	hypothetical protein	A0A0A7AQX9	Bacillus_phage	1.4e-43	87.1
WP_149216321.1\|2425994_2426339_+\|head,tail	head-tail adaptor protein	A0A0A7AR32	Bacillus_phage	4.2e-57	91.2
WP_000061895.1\|2426313_2426721_+	HK97 gp10 family phage protein	A0A0A7AQU9	Bacillus_phage	6.5e-65	94.1
WP_149216322.1\|2426726_2427089_+	hypothetical protein	A0A0S2MV90	Bacillus_phage	3.9e-61	95.8
WP_000729988.1\|2427103_2427697_+	hypothetical protein	A0A0S2MV81	Bacillus_phage	6.3e-109	99.5
WP_149216323.1\|2427743_2428172_+	hypothetical protein	A0A0S2MV94	Bacillus_phage	5.0e-68	94.4
WP_000375621.1\|2428276_2428483_+	hypothetical protein	A0A0S2MV68	Bacillus_phage	2.1e-32	98.5
WP_149216324.1\|2431174_2431939_+	hypothetical protein	A0A2R3ZXV6	Staphylococcus_phage	3.1e-20	34.3
WP_149216325.1\|2432247_2433729_+\|tail	phage tail family protein	A0A0S2MV63	Bacillus_phage	5.5e-263	88.0
WP_149216326.1\|2433725_2438039_+	hypothetical protein	A0A0S2MVB4	Bacillus_phage	0.0e+00	61.4
WP_000354139.1\|2438055_2438433_+	hypothetical protein	A0A0S2MVE0	Bacillus_phage	3.9e-40	62.9
WP_149216327.1\|2438560_2438785_+	hypothetical protein	A0A1B1P780	Bacillus_phage	9.5e-26	86.5
WP_098281123.1\|2438860_2439286_+\|holin	phage holin family protein	A0A1B1P787	Bacillus_phage	2.7e-69	94.3
WP_149216328.1\|2439285_2440104_+	N-acetylmuramoyl-L-alanine amidase	D2XR33	Bacillus_phage	5.7e-145	86.8
WP_149216329.1\|2440522_2441902_-	PQQ-binding-like beta-propeller repeat protein	NA	NA	NA	NA
WP_149216330.1\|2442096_2442288_-\|coat	spore coat protein	NA	NA	NA	NA
WP_149216331.1\|2442512_2442725_+	small, acid-soluble spore protein, alpha/beta type	A0A1P8CX76	Bacillus_phage	6.4e-16	70.3

DBSCAN-SWA_5

2483118 : 2488343

Bacillus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_001139345.1\|2483118_2483334_-	spore germination protein	A0A2H4J3A3	uncultured_Caudovirales_phage	3.4e-25	83.8
WP_000369348.1\|2483570_2483702_+	DUF3983 domain-containing protein	A0A1B1P7V8	Bacillus_phage	2.9e-11	79.1
WP_033685795.1\|2484066_2484855_+	C1 family peptidase	A0A2K9L1Z4	Tupanvirus	1.8e-34	36.5
WP_002005147.1\|2484874_2485087_+	hypothetical protein	NA	NA	NA	NA
WP_002061469.1\|2485151_2485352_+	N-acetylmuramoyl-L-alanine amidase	D2XR33	Bacillus_phage	1.0e-15	90.9
WP_097796955.1\|2485489_2486371_-	serine/threonine protein kinase	NA	NA	NA	NA
WP_149216344.1\|2486498_2487008_+	GrpB family protein	A0A2K5B2B6	Erysipelothrix_phage	4.1e-16	35.4
WP_000411453.1\|2487125_2487656_+	hypothetical protein	NA	NA	NA	NA
WP_149216345.1\|2487668_2488343_+	response regulator	W8CYM9	Bacillus_phage	2.8e-28	30.7

DBSCAN-SWA_6

3563143 : 3673040

109

Bacillus_phage(50.0%)

protease,head,tRNA,portal,integrase,coat,capsid,bacteriocin,terminase,tail

attL 3551422:3551439|attR 3675100:3675117

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
3551422:3551439	attL	NA	NA	NA	NA
WP_001288799.1\|3563143_3563686_-\|coat	outer spore coat protein CotE	NA	NA	NA	NA
WP_000870461.1\|3563811_3564243_-	hypothetical protein	NA	NA	NA	NA
WP_149216558.1\|3564246_3565776_-\|tRNA	tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB	NA	NA	NA	NA
WP_000190155.1\|3566206_3567073_-	2-oxoacid:ferredoxin oxidoreductase subunit beta	NA	NA	NA	NA
WP_000625417.1\|3567059_3568817_-	2-oxoacid:acceptor oxidoreductase subunit alpha	NA	NA	NA	NA
WP_065703380.1\|3569041_3569965_-	membrane dipeptidase	NA	NA	NA	NA
WP_000404341.1\|3570023_3570284_-	stage V sporulation protein SpoVS	J9PTX7	Bacillus_phage	8.4e-10	43.8
WP_001221095.1\|3570433_3571228_-	TIGR00282 family metallophosphoesterase	NA	NA	NA	NA
WP_000204911.1\|3571390_3572953_-	ribonuclease Y	NA	NA	NA	NA
WP_001283860.1\|3573389_3574421_-	recombinase RecA	A0A0S2MVG1	Bacillus_phage	1.1e-137	74.0
WP_149216559.1\|3574564_3575803_-	competence/damage-inducible protein A	NA	NA	NA	NA
WP_001052967.1\|3575823_3576402_-	CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase	NA	NA	NA	NA
WP_149216560.1\|3576466_3577378_-	DUF4115 domain-containing protein	NA	NA	NA	NA
WP_000574107.1\|3577399_3578185_-	DUF3388 domain-containing protein	NA	NA	NA	NA
WP_000114450.1\|3578323_3578572_-	DUF3243 domain-containing protein	NA	NA	NA	NA
WP_149216561.1\|3578647_3579361_-	SDR family oxidoreductase	NA	NA	NA	NA
WP_149216562.1\|3579461_3580748_-	insulinase family protein	A0A2H4UVM3	Bodo_saltans_virus	1.7e-10	28.9
WP_000772405.1\|3580748_3582023_-	insulinase family protein	A0A1X9I714	Streptococcus_phage	1.2e-56	30.1
WP_000008857.1\|3582232_3583192_-	ABC transporter permease	NA	NA	NA	NA
WP_001085254.1\|3583192_3584251_-	ABC transporter permease	NA	NA	NA	NA
WP_000456908.1\|3584243_3585776_-	ABC transporter ATP-binding protein	W6JKT0	Anomala_cuprea_entomopoxvirus	2.8e-12	26.6
WP_000725771.1\|3585893_3586979_-	BMP family ABC transporter substrate-binding protein	NA	NA	NA	NA
WP_000114182.1\|3587071_3587797_-	GntR family transcriptional regulator	NA	NA	NA	NA
WP_001118776.1\|3588334_3590716_-	DNA translocase FtsK	A0A218M9A2	Mycobacterium_phage	2.0e-89	51.4
WP_000605020.1\|3590928_3591132_-	ribonuclease	NA	NA	NA	NA
WP_000139806.1\|3591128_3591878_-	translocation-enhancing protein TepA	NA	NA	NA	NA
WP_000823096.1\|3591980_3593651_-	ribonuclease J	NA	NA	NA	NA
WP_000564764.1\|3594387_3595266_-	4-hydroxy-tetrahydrodipicolinate synthase	NA	NA	NA	NA
WP_000692453.1\|3595277_3596510_-	aspartate kinase	NA	NA	NA	NA
WP_000414846.1\|3596533_3597580_-	aspartate-semialdehyde dehydrogenase	NA	NA	NA	NA
WP_149216766.1\|3597730_3597973_-	dipicolinate synthase	NA	NA	NA	NA
WP_149216563.1\|3598158_3599814_+	hypothetical protein	NA	NA	NA	NA
WP_000272778.1\|3599834_3600245_+	hypothetical protein	NA	NA	NA	NA
WP_004406814.1\|3600476_3600602_-	hypothetical protein	A0A1B1P878	Bacillus_phage	4.0e-10	75.0
WP_098869208.1\|3600666_3601128_+	hypothetical protein	NA	NA	NA	NA
WP_000245563.1\|3601155_3601383_+	hypothetical protein	NA	NA	NA	NA
WP_149216564.1\|3601407_3601833_+	SMI1/KNR4 family protein	NA	NA	NA	NA
WP_149216565.1\|3602399_3603209_-	N-acetylmuramoyl-L-alanine amidase	D2XR33	Bacillus_phage	2.6e-134	83.3
WP_001261076.1\|3603208_3603445_-\|bacteriocin	bacteriocin biosynthesis protein	A0A2H4JGN9	uncultured_Caudovirales_phage	1.7e-25	100.0
WP_097999696.1\|3603475_3603850_-	hypothetical protein	A0A0S2MVE0	Bacillus_phage	4.6e-65	97.6
WP_149216566.1\|3603865_3608185_-	hypothetical protein	A0A0S2MVB4	Bacillus_phage	0.0e+00	61.7
WP_149216567.1\|3608181_3609657_-\|tail	phage tail family protein	A0A0S2MV63	Bacillus_phage	1.0e-237	80.7
WP_149216568.1\|3609698_3613325_-	DUF2207 domain-containing protein	A0A2H4JAI2	uncultured_Caudovirales_phage	1.5e-184	86.5
WP_000415905.1\|3613557_3613920_-	hypothetical protein	A0A2H4JAG8	uncultured_Caudovirales_phage	1.2e-41	70.0
WP_001004907.1\|3613924_3614512_-\|tail	phage tail protein	A0A2H4JDV4	uncultured_Caudovirales_phage	5.1e-87	82.1
WP_000176452.1\|3614512_3614848_-	hypothetical protein	D2XR22	Bacillus_phage	7.5e-51	89.9
WP_149216569.1\|3614844_3615189_-	HK97 gp10 family phage protein	A0A2H4JAH8	uncultured_Caudovirales_phage	3.7e-45	79.6
WP_149216570.1\|3615190_3615544_-\|head	phage head closure protein	A0A2H4JGG7	uncultured_Caudovirales_phage	5.6e-57	92.2
WP_149216571.1\|3615545_3615839_-	hypothetical protein	A0A2H4JG04	uncultured_Caudovirales_phage	1.1e-42	86.6
WP_149216572.1\|3615844_3616996_-\|capsid	phage major capsid protein	A0A2H4JHU0	uncultured_Caudovirales_phage	1.1e-207	95.8
WP_048558800.1\|3616999_3617743_-\|protease	Clp protease ClpP	D2XR17	Bacillus_phage	1.4e-105	81.4
WP_149216573.1\|3617742_3618888_-\|portal	phage portal protein	A0A2H4JAJ1	uncultured_Caudovirales_phage	1.5e-180	81.6
WP_149216574.1\|3618896_3620564_-\|terminase	terminase large subunit	D2XR15	Bacillus_phage	8.5e-305	90.5
WP_149216575.1\|3620560_3620872_-\|terminase	P27 family phage terminase small subunit	D2XR14	Bacillus_phage	2.3e-46	97.1
WP_149216576.1\|3620994_3621330_-	HNH endonuclease	D2XR61	Bacillus_phage	4.0e-52	90.1
WP_149216577.1\|3622021_3622903_-	hypothetical protein	NA	NA	NA	NA
WP_149216578.1\|3622924_3624016_-	hypothetical protein	NA	NA	NA	NA
WP_060750964.1\|3624705_3624906_+	cold-shock protein	Q9AZD3	Lactococcus_phage	1.1e-20	72.7
WP_080483135.1\|3625208_3625388_+	gamma-type small acid-soluble spore protein	NA	NA	NA	NA
WP_001012165.1\|3625796_3626339_-\|integrase	site-specific integrase	W8CYP1	Bacillus_phage	2.5e-88	91.7
WP_149216579.1\|3626338_3626803_-	ArpU family transcriptional regulator	D2XR57	Bacillus_phage	5.1e-74	92.9
WP_149216580.1\|3628184_3629423_+	NTTRR-F1 domain	NA	NA	NA	NA
WP_086389448.1\|3629939_3630131_-	DUF3954 domain-containing protein	D2XR48	Bacillus_phage	8.3e-23	88.5
WP_149216581.1\|3630205_3630568_-	cell division protein SepF	D2XR47	Bacillus_phage	2.7e-54	89.2
WP_089148379.1\|3630542_3630731_-	hypothetical protein	D2XR45	Bacillus_phage	3.7e-15	83.6
WP_149216582.1\|3630733_3632056_-	replicative DNA helicase	D2XR44	Bacillus_phage	1.9e-235	95.0
WP_149216583.1\|3632052_3633000_-	DnaD domain protein	D2XR43	Bacillus_phage	2.4e-86	61.4
WP_149216584.1\|3633001_3633187_-	hypothetical protein	NA	NA	NA	NA
WP_149216585.1\|3633279_3633564_-	hypothetical protein	D2XR42	Bacillus_phage	1.1e-21	52.1
WP_086407747.1\|3633752_3634067_+	hypothetical protein	NA	NA	NA	NA
WP_086389452.1\|3634397_3634619_-	RNA polymerase subunit sigma	NA	NA	NA	NA
WP_000537277.1\|3634632_3635220_-	hypothetical protein	D2XR41	Bacillus_phage	2.2e-74	69.7
WP_002082295.1\|3635310_3635559_-	hypothetical protein	NA	NA	NA	NA
WP_065845234.1\|3635611_3635800_-	helix-turn-helix transcriptional regulator	A0A2H4J4N8	uncultured_Caudovirales_phage	4.2e-11	55.9
WP_063548461.1\|3635955_3636390_+	helix-turn-helix transcriptional regulator	D2XR39	Bacillus_phage	7.2e-30	53.9
WP_086389454.1\|3636402_3636837_+	ImmA/IrrE family metallo-endopeptidase	D2XR38	Bacillus_phage	3.9e-60	77.8
WP_149216586.1\|3636877_3637432_+	hypothetical protein	NA	NA	NA	NA
WP_149216587.1\|3637465_3638197_+	hypothetical protein	NA	NA	NA	NA
WP_149216588.1\|3638399_3639947_+	recombinase family protein	I3VYZ3	Thermoanaerobacterium_phage	1.6e-143	53.8
WP_000954735.1\|3640401_3641304_-	dipicolinic acid synthetase subunit A	NA	NA	NA	NA
WP_001239756.1\|3641474_3641726_-	YlmC/YmxH family sporulation protein	NA	NA	NA	NA
WP_000592989.1\|3641860_3643102_-	insulinase family protein	G3MBJ8	Bacillus_virus	4.4e-56	34.5
WP_000868232.1\|3643188_3644088_-	polysaccharide deacetylase family protein	NA	NA	NA	NA
WP_000076737.1\|3644241_3646380_-	polyribonucleotide nucleotidyltransferase	NA	NA	NA	NA
WP_001229392.1\|3646540_3646810_-	30S ribosomal protein S15	NA	NA	NA	NA
WP_000766704.1\|3646910_3647882_-	bifunctional riboflavin kinase/FAD synthetase	A0A1V0SD03	Indivirus	2.5e-06	32.7
WP_087967423.1\|3647925_3648849_-\|tRNA	tRNA pseudouridine(55) synthase TruB	NA	NA	NA	NA
WP_000776437.1\|3648935_3649292_-	30S ribosome-binding factor RbfA	NA	NA	NA	NA
WP_000582367.1\|3649307_3649589_-	DUF503 family protein	NA	NA	NA	NA
WP_000036343.1\|3649585_3651646_-	translation initiation factor IF-2	A0A2H4UTS4	Bodo_saltans_virus	3.8e-20	25.9
WP_001286524.1\|3651650_3651962_-	YlxQ family RNA-binding protein	NA	NA	NA	NA
WP_000071127.1\|3651962_3652235_-	YlxR family protein	NA	NA	NA	NA
WP_000102604.1\|3652246_3653353_-	transcription termination/antitermination protein NusA	NA	NA	NA	NA
WP_000359096.1\|3653370_3653841_-	ribosome maturation factor RimP	NA	NA	NA	NA
WP_149216589.1\|3654177_3658479_-	PolC-type DNA polymerase III	A0A0A8WJ41	Clostridium_phage	4.1e-24	39.6
WP_149216590.1\|3658603_3660304_-\|tRNA	proline--tRNA ligase	NA	NA	NA	NA
WP_001090243.1\|3660413_3661670_-\|protease	RIP metalloprotease RseP	NA	NA	NA	NA
WP_000790372.1\|3661687_3662830_-	1-deoxy-D-xylulose-5-phosphate reductoisomerase	NA	NA	NA	NA
WP_000813592.1\|3662853_3663645_-	phosphatidate cytidylyltransferase	NA	NA	NA	NA
WP_149216591.1\|3663662_3664439_-	isoprenyl transferase	R9W0U9	Flavobacterium_phage	9.0e-23	42.5
WP_000531501.1\|3664524_3665082_-	ribosome recycling factor	NA	NA	NA	NA
WP_000042668.1\|3665084_3665807_-	UMP kinase	NA	NA	NA	NA
WP_149216592.1\|3665873_3666761_-	elongation factor Ts	NA	NA	NA	NA
WP_000111485.1\|3666864_3667566_-	30S ribosomal protein S2	NA	NA	NA	NA
WP_000421290.1\|3667914_3668694_-	GTP-sensing pleiotropic transcriptional regulator CodY	NA	NA	NA	NA
WP_000550087.1\|3668771_3670163_-\|protease	ATP-dependent protease ATPase subunit HslU	A0A173GFL6	Erwinia_phage	3.2e-47	30.0
WP_048567505.1\|3670185_3670728_-\|protease	ATP-dependent protease proteolytic subunit HslV	NA	NA	NA	NA
WP_001101241.1\|3670770_3671670_-	tyrosine recombinase XerC	A0A0K2CP59	Brevibacillus_phage	3.1e-35	31.8
WP_000213002.1\|3671735_3673040_-\|tRNA	FADH(2)-oxidizing methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))- methyltransferase TrmFO	NA	NA	NA	NA
3675100:3675117	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_CP031070

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP031070_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP031070_1

181202-181598

TypeV

Consensus_repeat	Method
ATCTCCTGGACTACCGAAATAGTATTGCTTNCCGTNAANT	CRT

4 spacers

cas14j

The CRISPR arrays of NZ_CP031070_1

>merge|NZ_CP031070|1|181202-181598|CRT
ATCTCCTGGACTACCGAAATAGTATTGCTTTCCGTCAACTTTATACCAGCCAGTTTGCATTTTTCCCATTGCTTCTCTTTGTTCAGTTGTTGGATTGTTATAGAAAAGGAAATAGTATTGCTTCCCGTTAATTTGAAGAATTCCTTTTGCCATTTCTCCAGTTTCTAAGCGACGATCAGTAGTATCTCCTGGACTACCGAAATAGTATTGCTCCCCGTTAAGGGATTTCCAGCCTGTTGCTATTTCTCCGATTTCTAAGCCAGTACCATCTCCTGGACTACCGAAATAGTATTGCTTTTCGTTAATTTGAAGAATTCCTTTTGCCATTTCTCCAGTTTCTAAGCGACGATCAGTAGTATCTCCTGGACTACCGAAATAGTATTGCTTCCCATCAATT

>NZ_CP031070|1|1|181202-181598|CRT
ATCTCCTGGACTACCGAAATAGTATTGCTTTCCGTCAACT	TTATACCAGCCAGTTTGCATTTTTCCCATTGCTTCTCTTTGTTCAGTTGTTGG
ATTGTTATAGAAAAGGAAATAGTATTGCTTCCCGTTAATT	TGAAGAATTCCTTTTGCCATTTCTCCAGTTTCTAAGCGACGATCAGTAGT
ATCTCCTGGACTACCGAAATAGTATTGCTCCCCGTTAAGG	GATTTCCAGCCTGTTGCTATTTCTCCGATTTCTAAGCCAGTACC
ATCTCCTGGACTACCGAAATAGTATTGCTTTTCGTTAATT	TGAAGAATTCCTTTTGCCATTTCTCCAGTTTCTAAGCGACGATCAGTAGT
ATCTCCTGGACTACCGAAATAGTATTGCTTCCCATCAATT

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP031070.1\|WP_149217184.1\|184171_184294_+\|transposase	unknown	unknown	gnl\|CDD\|376773
NZ_CP031070.1\|WP_149217188.1\|188785_190114_-\|magnesium-transporter	unknown	unknown	gnl\|CDD\|225148
NZ_CP031070.1\|WP_149217186.1\|185692_186499_-\|N-acetylmuramoyl-L-alanine-amidase	unknown	unknown	gnl\|CDD\|119383
NZ_CP031070.1\|WP_149217173.1\|163682_164306_+\|DUF1361-domain-containing-protein	unknown	unknown	gnl\|CDD\|369208
NZ_CP031070.1\|WP_149217187.1\|186983_187400_-\|phage-holin-family-protein	unknown	unknown	gnl\|CDD\|377457
NZ_CP031070.1\|WP_149217181.1\|173204_174596_+\|amino-acid-permease	unknown	unknown	gnl\|CDD\|129987
NZ_CP031070.1\|WP_149217191.1\|194233_195046_+\|polysaccharide-deacetylase-family-protein	unknown	unknown	gnl\|CDD\|200569
NZ_CP031070.1\|WP_149217177.1\|166932_168144_+\|IS110-family-transposase	unknown	unknown	gnl\|CDD\|376773
NZ_CP031070.1\|WP_149217175.1\|164825_165356_-\|DUF3967-domain-containing-protein	unknown	unknown	gnl\|CDD\|289896
NZ_CP031070.1\|WP_149217179.1\|171004_172117_+\|alanine-dehydrogenase	unknown	unknown	gnl\|CDD\|240630
NZ_CP031070.1\|WP_149217185.1\|184805_185417_-\|sporulation-membrane-protein-YtaF	unknown	unknown	gnl\|CDD\|274321
NZ_CP031070.1\|WP_149217176.1\|165726_166446_+\|MOSC-domain-containing-protein	unknown	unknown	gnl\|CDD\|225758
NZ_CP031070.1\|WP_149217180.1\|172113_173115_+\|bifunctional-threonine-ammonia-lyase/L-serine-ammonia-lyase-TdcB	unknown	unknown	gnl\|CDD\|236317
NZ_CP031070.1\|WP_149217189.1\|190998_192459_+\|HTH-domain-containing-protein	unknown	unknown	gnl\|CDD\|368260
NZ_CP031070.1\|WP_149217182.1\|178195_179713_-\|lethal-factor-domain-protein	unknown	unknown	gnl\|CDD\|380335
NZ_CP031070.1\|WP_149217174.1\|164305_164599_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP031070.1\|WP_149217192.1\|195382_195787_-\|phosphate-starvation-inducible-protein-PsiE	unknown	unknown	gnl\|CDD\|235075
NZ_CP031070.1\|WP_149217242.1\|185564_185696_-\|N-acetylmuramoyl-L-alanine-amidase	unknown	unknown	unknown
NZ_CP031070.1\|WP_149217190.1\|192455_193565_+\|helix-turn-helix-domain-containing-protein	cas14j	-	gnl\|CDD\|223747
NZ_CP031070.1\|WP_149217178.1\|169025_170375_-\|amino-acid-permease	unknown	unknown	gnl\|CDD\|273332

Protein	Function_ID	Function_description	E-value
NZ_CP031070.1\|WP_149217184.1\|184171_184294_+\|transposase	gnl\|CDD\|376773	pfam02371, Transposase_20, Transposase IS116/IS110/IS902 family. Transposases are needed for efficient transposition of the insertion sequence or transposon DNA. This family includes transposases for IS116, IS110 and IS902. This region is often found with pfam01548. The exact function of this region is uncertain. This family contains a HHH motif suggesting a DNA-binding function.	9.37132e-05
NZ_CP031070.1\|WP_149217186.1\|185692_186499_-\|N-acetylmuramoyl-L-alanine-amidase	gnl\|CDD\|119383	cd06523, GH25_PlyB-like, PlyB is a bacteriophage endolysin that displays potent lytic activity toward Bacillus anthracis. PlyB has an N-terminal glycosyl hydrolase family 25 (GH25) catalytic domain and a C-terminal bacterial SH3-like domain, SH3b. Both domains are required for effective catalytic activity. Endolysins are produced by bacteriophages at the end of their life cycle and participate in lysing the bacterial cell in order to release the newly formed progeny. Endolysins (also referred to as endo-N-acetylmuramidases or peptidoglycan hydrolases) degrade bacterial cell walls by catalyzing the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues.	3.0294e-53
NZ_CP031070.1\|WP_149217173.1\|163682_164306_+\|DUF1361-domain-containing-protein	gnl\|CDD\|369208	pfam07099, DUF1361, Protein of unknown function (DUF1361). This family consists of several hypothetical bacterial proteins of around 200 residues in length. The function of this family is unknown although some members are annotated as being putative integral membrane proteins.	2.15358e-21
NZ_CP031070.1\|WP_149217187.1\|186983_187400_-\|phage-holin-family-protein	gnl\|CDD\|377457	pfam05105, Phage_holin_4_1, Bacteriophage holin family. Phage holins and lytic enzymes are both necessary for bacterial lysis and virus dissemination. This family also includes TcdE/UtxA involved in toxin secretion in Clostridium difficile. The 1.E.10 family is represented by Bacillus subtilis phi29 holin; 1.E.16 represents the Cph1 holin; and the 1.E.19 family is represented by the Clostridium difficile TcdE holin. Toxigenic strains of C. difficile produce two large toxins (TcdA and TcdB) encoded within a pathogenicity locus. tcdE, encoded between tcdA and tcdB, encodes a 166 aa protein which causes death to E. coli when expressed, and the structure of TcdE resembles holins. TcdE acts on the bacterial membrane. Since TcdA and TcdB lack signal peptides, they may be released via TcdE either prior to or subsequent to cell lysis.	5.23871e-37
NZ_CP031070.1\|WP_149217182.1\|178195_179713_-\|lethal-factor-domain-protein	gnl\|CDD\|380335	cd20185, M34_PABD, N-terminal protective antigen-binding domain (PABD) of Anthrax Toxin Lethal Factor (ATLF) and Edema Factor (EF), and similar domains; belongs to peptidase family M34. This subfamily includes the functional N-terminal protective antigen-binding domain (PABD) of the anthrax edema factor (EF), as well as the likely inactive N-terminal PABD of anthrax toxin lethal factor (ATLF), both secreted by Bacillus anthracis. ATLF and EF are enzyme components of anthrax toxin and are carried into the cell by a third component, the protective antigen (PA). ATLF-PABD resembles the C-terminal catalytic domain of ATLF (EC 3.4.24.83) but lacks the hallmark metalloprotease motif HEXXH. This subfamily belongs to the peptidase family M34 (also known as the anthrax lethal factor family), which also includes the C-terminal catalytic domain of ATLF, and Pro-Pro endopeptidase (PPEP-1; EC 3.4.24.89, also known as Zmp1), which is an extracellular metalloprotease that shows a unique specificity for hydrolyzing a Pro-Pro bond and is involved in bacterial adhesion.	5.30124e-70
NZ_CP031070.1\|WP_149217191.1\|194233_195046_+\|polysaccharide-deacetylase-family-protein	gnl\|CDD\|200569	cd10944, CE4_SmPgdA_like, Catalytic NodB homology domain of Streptococcus mutans polysaccharide deacetylase PgdA, Bacillus subtilis YheN, and similar proteins. This family is represented by a putative polysaccharide deacetylase PgdA from the oral pathogen Streptococcus mutans (SmPgdA) and Bacillus subtilis YheN (BsYheN), which are members of the carbohydrate esterase 4 (CE4) superfamily. SmPgdA is an extracellular metal-dependent polysaccharide deacetylase with a typical CE4 fold, with metal bound to a His-His-Asp triad. It possesses de-N-acetylase activity toward a hexamer of chitooligosaccharide N-acetylglucosamine, but not shorter chitooligosaccharides or a synthetic peptidoglycan tetrasaccharide. SmPgdA plays a role in tuning cell surface properties and in interactions with (salivary) agglutinin, an essential component of the innate immune system, most likely through deacetylation of an as-yet-unidentified polysaccharide. SmPgdA shows significant homology to the catalytic domains of peptidoglycan deacetylases from Streptococcus pneumoniae (SpPgdA) and Listeria monocytogenes (LmPgdA), both of which are involved in the bacterial defense mechanism against human mucosal lysozyme. The Bacillus subtilis genome contains six polysaccharide deacetylase gene homologs: pdaA, pdaB (previously known as ybaN), yheN, yjeA, yxkH and ylxY. The biological function of BsYheN is still unknown. This family also includes many uncharacterized polysaccharide deacetylases mainly found in bacteria.	9.78586e-84
NZ_CP031070.1\|WP_149217177.1\|166932_168144_+\|IS110-family-transposase	gnl\|CDD\|376773	pfam02371, Transposase_20, Transposase IS116/IS110/IS902 family. Transposases are needed for efficient transposition of the insertion sequence or transposon DNA. This family includes transposases for IS116, IS110 and IS902. This region is often found with pfam01548. The exact function of this region is uncertain. This family contains a HHH motif suggesting a DNA-binding function.	1.65309e-24
NZ_CP031070.1\|WP_149217175.1\|164825_165356_-\|DUF3967-domain-containing-protein	gnl\|CDD\|289896	pfam13152, DUF3967, Protein of unknown function (DUF3967). This family of proteins is functionally uncharacterized. This family of proteins is found in bacteria. Proteins in this family are typically between 173 and 249 amino acids in length.	3.66021e-08
NZ_CP031070.1\|WP_149217179.1\|171004_172117_+\|alanine-dehydrogenase	gnl\|CDD\|240630	cd05305, L-AlaDH, Alanine dehydrogenase NAD-binding and catalytic domains. Alanine dehydrogenase (L-AlaDH) catalyzes the NAD-dependent conversion of pyruvate to L-alanine via reductive amination. Like formate dehydrogenase and related enzymes, L-AlaDH is comprised of 2 domains connected by a long alpha helical stretch, each resembling a Rossmann fold NAD-binding domain. The NAD-binding domain is inserted within the linear sequence of the more divergent catalytic domain. Ligand binding and active site residues are found in the cleft between the subdomains. L-AlaDH is typically hexameric and is critical in carbon and nitrogen metabolism in micro-organisms.	0
NZ_CP031070.1\|WP_149217188.1\|188785_190114_-\|magnesium-transporter	gnl\|CDD\|225148	COG2239, MgtE, Mg/Co/Ni transporter MgtE (contains CBS domain) [Inorganic ion transport and metabolism].	1.14123e-131
NZ_CP031070.1\|WP_149217176.1\|165726_166446_+\|MOSC-domain-containing-protein	gnl\|CDD\|225758	COG3217, COG3217, Uncharacterized Fe-S protein [General function prediction only].	9.50171e-25
NZ_CP031070.1\|WP_149217180.1\|172113_173115_+\|bifunctional-threonine-ammonia-lyase/L-serine-ammonia-lyase-TdcB	gnl\|CDD\|236317	PRK08638, PRK08638, bifunctional threonine ammonia-lyase/L-serine ammonia-lyase TdcB.	0
NZ_CP031070.1\|WP_149217189.1\|190998_192459_+\|HTH-domain-containing-protein	gnl\|CDD\|368260	pfam05043, Mga, Mga helix-turn-helix domain. M regulator protein trans-acting positive regulator (Mga) is a DNA-binding protein that activates the expression of several important virulence genes in group A streptococcus in response to changing environmental conditions. This domain is found in the centre of the Mga proteins. This family also contains a number of bacterial RofA transcriptional regulators that seem to be largely restricted to streptococci. These proteins have been shown to regulate the expression of important bacterial adhesins. This is presumably a DNA-binding domain.	3.17245e-09
NZ_CP031070.1\|WP_149217185.1\|184805_185417_-\|sporulation-membrane-protein-YtaF	gnl\|CDD\|274321	TIGR02840, conserved_hypothetical_protein, putative sporulation protein YtaF. This protein family was identified, at the time of the publication of the Carboxydothermus hydrogenoformans genome, as having a phylogenetic profile that exactly matches the subset of the Firmicutes capable of forming endospores. The species include Bacillus anthracis, Clostridium tetani, Thermoanaerobacter tengcongensis, Geobacillus kaustophilus, etc. This protein, previously named YtaF, is therefore a putative sporulation protein. [Cellular processes, Sporulation and germination].	1.58805e-29
NZ_CP031070.1\|WP_149217181.1\|173204_174596_+\|amino-acid-permease	gnl\|CDD\|129987	TIGR00909, putative_amino_acid_transporter, amino acid transporter. [Transport and binding proteins, Amino acids, peptides and amines].	4.80477e-137
NZ_CP031070.1\|WP_149217192.1\|195382_195787_-\|phosphate-starvation-inducible-protein-PsiE	gnl\|CDD\|235075	PRK02833, PRK02833, phosphate-starvation-inducible protein PsiE; Provisional.	1.11193e-38
NZ_CP031070.1\|WP_149217190.1\|192455_193565_+\|helix-turn-helix-domain-containing-protein	gnl\|CDD\|223747	COG0675, COG0675, Transposase and inactivated derivatives [DNA replication, recombination, and repair].	1.56681e-47
NZ_CP031070.1\|WP_149217178.1\|169025_170375_-\|amino-acid-permease	gnl\|CDD\|273332	TIGR00911, High-affinity_methionine_permease, L-type amino acid transporter. [Transport and binding proteins, Amino acids, peptides and amines].	2.07951e-68

>NZ_CP031070.1|WP_149217182.1|178195_179713_-|lethal-factor-domain-protein
MQVKNKFLFKVFSTVSVISLLSVTFATFPKSTYAINKGHIEEKEKEKQKEKDEKKEKNKEEKEKEFIKDIVKPDYTSEKKEMAQQTEQMLEKIPHEVLEMYDKIGGKIYITDKELTKQDELKNLSEKEKNVIDSNGKPASLDSRFVFAKGGKNPALIIRIEDYQNDNEKRKEVYYELGKEIIRDVLNEKELEGEPFIKAVNKANEDSDSRDLLFGESLQKELKSDNKPIDKEFIKKHLTAFQDVFAQAFSQYFESEHKESLKLYAPDMFKYIEELDQKRFKEINDSMENKKNSEENNKDKTIKDFSRDIKAADIWYKEVFKDFNNKLNPEQKASIQMYTTQNYKTINKGLRESNLPEDKEKEVKNISEALNKSSLPEKVVVYRKTGKDSLGLEINTNFKDQKVIENLKNKLEHSTREEKAFLSTSIANHFSESFDVKSVLYKITVPKGTKAAYIFGDLATYQGESELLIDKGYSYEITKISTYEHTKNTGTKQTNLLVEATLLPK
>NZ_CP031070.1|WP_149217181.1|173204_174596_+|amino-acid-permease
MINLLRKKSVTQLLGESQSKTLTKTLGAFDLTMLGIGAIIGTGVLVLTGLVAARDAGPAVIFSFMIAAIVCGFAALCYAEVASTLPVSGSVYTYSYATIGEFVAHLMGWTLLAVYAVTTAAVAGGWTGYFQNLVSGFSLEIPKALLTIPSQGGIVNLPAVIIILVLTWLLSRGTKESKRVNNAMVLIKISIIVLFIVVGVFYVKPENWVPFAPYGLSGIFAGGAAVFFSFLGFDALATSAEEVKNPQRDLPIGIITSLVICTIIYIVVCLVMTGMVSYKELDVPEAMAYVLAAIGQDKVAGVIAVGAIIGIMAVIFAYIYATTRVFFAMSRDGLLPKSFVKINKKTEAPTFSTWLTGIGSALIAGFIDLKELSNLANIGALLTFAMVGVSVIILRKKHPKLKRGFMVPLVPILPIISIACCLFLMVNLPLRTWIYFGAWLAIGVVVYFVYSKKHSHLTKEENS
>NZ_CP031070.1|WP_149217180.1|172113_173115_+|bifunctional-threonine-ammonia-lyase/L-serine-ammonia-lyase-TdcB
MINKSLPLNIGDIKKAQQILFGKARKTPLVKSFYLTSKTGGEVYLKLENMQLTGSFKFRGAFNKISNLTDEEKARGVIACSAGNHAQGVALSSHLLGIKSKIVMPTSAPQAKVDATRGYGSEVILHGDTFDDAKVKCEEIIQETGETYLHPYDDAEVMAGQGTIGLDILDDMWDVDTVIVPIGGGGIISGIAVALKSFNPSINIIGIQAANFHGMKASYDVGKIVSHYEAPTIADGCAVKIPGNLTFEIVKELVDDIVTVSEDELEVAMKDLLQRGKAVVEGAGALATAALLAGKVDQYIKDKKVVAVISGGNVDLKRISSVCEHFFVANEVK
>NZ_CP031070.1|WP_149217179.1|171004_172117_+|alanine-dehydrogenase
MKIGVVKEIKKGEARVGMTPENVRKLVEEGNQVLVQKDAGLGSGYTNDEYANAGASLVTQAEAWDVDMVVKVKEPLPAEYHYFKKDLIVWGFLHLAASKECVETMKQVGTTAIAGETISNNGVLALLKPMSAIAGRRAVFMGAYYLEKQHQGEGILLSGIEGIHAGNVVILGGGNAAINACDMAIGIGCHVTILELNKTQIAYLKEKYANGSVEVIESNTKNLEKHIKEADLFISTILIPGAKPPKIVKEYMVQSMKPGSVIVDIAIDQGGTVETVDHYTTHDNPIFIKHDVIHYAVPNMPGATPRTSTMALANGNIEYLLAIAKDGLENAINIKPALASGINIYKGTITYENLGSTLELEYIPLKEVLI
>NZ_CP031070.1|WP_149217178.1|169025_170375_-|amino-acid-permease
MSQQQLKKEIGFLAALTTVIGTVIGAGVFFKPTALYGVTGTASLGLLAWVIGGILTICAGLTAAELSAAIPETGGMMAYLKRTYGNLTAFLLGWAQTVIYFPANIAALAIIFGTQTVSLFGLDANENKMMIVGIAVVTATFVTLMNFLGAKTAGGIQMVSTICKLFPLALIIIFGLLHKGDVPFQLLPIEAGANKSFISALGSGLLATMFAYDGWIHVGNIAGEMKNPKRDLPKAIILGLSIIMVVYLLINIAFLMVMSATALAGTDTPASEVVTIIFGNMGGKLVSIGILISVFGTINGYILTGMRIPYAMALENKLPCSKWFATLSNKSRIPYNSGIFILLISVIMMLVGGFNTLTDMLVFVIWIFYTMTFLAVFILRKREPELIRPYKVPLYPFIPIISLLGGAFIVLNTLFTQPLLALCGIGLTGLGLPIYFKMRHKHTNVKVKN
>NZ_CP031070.1|WP_149217177.1|166932_168144_+|IS110-family-transposase
MQHVIAFDVSMGKSYMVIYNAQKQCIFESEINHSKPDFEELRKRIDQLMTENKKSPHILFEATGVYSRQLERFMQDNKFEYFLLNPLEAKLQCDSLRIHKTDRSDAHQLALTHFTASRREKTGTDNLFHQLKSLSRFYNELDDELSLIRSRMHKVIQLTFPELEQIFITKSDLFLNFVQLFPHPDFVRNLSKTVMKNQIRKNTNKNISIRVAEKKAMEVLEAARYSYPAVRQDDVLCEQLKSYAKRYQELLHQKEQCITKMVRLAESRTEYPIILSFPGIGPNTAIRLLAEIGDITRFKNNKQLNAFAGIDIRRFQSGKTFFKDKINKRGNKHLRKLLFLIIQNMIKLRRYGQNHIVEYYDKLKTQPYNKCHKVASIACVNKLLKLLFYLITHNIHYDYRLTA
>NZ_CP031070.1|WP_149217176.1|165726_166446_+|MOSC-domain-containing-protein
MIIGEIKEIVRHPIKSFRRENVQQTYIASYGLYGDRSHAFLDETRPGKYLTATQLPEMIGYTASFIGEESLDTYPPIKIVSPEGKIYKWGDQELLTELEQKSDRKIKDIQYSPQQVPLGAIEEEHILIVTDTSLQEMSTMWGQDVNHRRFRPNLIFSLYENIPFAEDTWFGKCIRIGEVGLKIVRHCERCMIITIDPNPLTLEKTLLKTVVQKRNNHFGVYASVIKPGKVNVGDSIVLE
>NZ_CP031070.1|WP_149217175.1|164825_165356_-|DUF3967-domain-containing-protein
MATEEHLGTEGGQNTAQTYWGSEVAKNLGIASSTLRKYCLALEEAGYPFDRGNNNSRIFYYKDVATIERLITAMNKKNVTPEQAINLAIASVEEDEIATVAIDSVADTEHIKVLSERIERLDQQSKLLQETDAQRIIREEQRDIQLMQVLREIQDSKRLVAVSKQKKSFWSRLFGK
>NZ_CP031070.1|WP_149217174.1|164305_164599_+|hypothetical-protein
METLLWTIGLVMLASQIYISVHLYKTEQSILWACIGFFLCFGLNVYIYQITKLEKYAGYSFEKLQQKERKKWQKVYVLILIQYIIYLLYLVGLTPHK
>NZ_CP031070.1|WP_149217173.1|163682_164306_+|DUF1361-domain-containing-protein
MNERSKARLSFFIYISLLIICDVKYSSFLILNMFLAFSALELSFLLPLFKIQEKRDIPSSLAFYGIFILLSPNVFYVVTDLIHLNIFKFDYKKELLLKEWWNFSILTAGVLLSIFYYTLMLKQIESLLGNFKYKQGALLMLILLSSFGIYIGRFLRFHSVHLFIEPFSLLIQVWKTLNIEALLFIGWISILQLIIYWLFVSIRKDEQ
>NZ_CP031070.1|WP_149217184.1|184171_184294_+|transposase
MSTLPQTLDSIPGVDPVFAADIVTEIGQIDFFVDETRIAK
>NZ_CP031070.1|WP_149217185.1|184805_185417_-|sporulation-membrane-protein-YtaF
MSTAGIFSIILIGISSNLDNAGVAIAYGMRKIQISWFHNLIIACFGFLFTLLGGIFGNWISLFISEFTANLIGAIVLGGIGILVLCQSLLSKDDQFHQKTEDGKRKILHQPEYNVLNSSKIIGLSETMILGIALSINNIAGGFDAGITNLNLWWTAFVSGIFSLMCMSIFSEIGKTFLAEYLGKWASVISSILLILIGMNQIL
>NZ_CP031070.1|WP_149217242.1|185564_185696_-|N-acetylmuramoyl-L-alanine-amidase
MEPSYSFIVSEKIKVDELSKYKVYNSNKQIFYITIKKLHNMAQ
>NZ_CP031070.1|WP_149217186.1|185692_186499_-|N-acetylmuramoyl-L-alanine-amidase
MGYIIDLSKWNDNIDWDVAAPQLDLAICRVQYGSNKKDTLYDAHVTNLEAKGIRHAAYAYGCYESVNDAIVEAKDFLARVHPNAKFLVLDVEDDTLKAMKDENKLAEASQAFIQTCRKAGWKIGLYVSHHMYDSHGLQNVDADFLWLPRYGTNDGKPQKKPSYSCDIWQYTDNGYINGIGKVDMNLLQGNKKLDWYVGEQAKYIATGGLGLKACTEISQYLLERRWWAIMEFTTNGDAFVKTGGICEPNLSEFREWMDQKGWWYEVKE
>NZ_CP031070.1|WP_149217187.1|186983_187400_-|phage-holin-family-protein
MDSINMLFKTVITTLGAFCGYFLGEWNTVLQILVSIVVIDYITGVIAAGYNGNLKSKIGFRGISKKVMLFLLVGVSAQLDTVFGSHSTIREATIFFFVGNELLSILENAGLMGIKLPNALIKAVEILNNQSKQNKGEK
>NZ_CP031070.1|WP_149217188.1|188785_190114_-|magnesium-transporter
MRKKIKQLLLTKNIFIAEDLLKIPPHDIAKEVKSFRFKDQVSLIDLMPAAKSSKILRYISPKERCAILNSLSEDKAKILLNSQPIDELVDLFVAINPDKTLKLMSYLSKEKQQKVRELMIYKPGTAGSLVTSDYITARENWSVKKTLEYIRKIAKDVESVSYIYVLDNHGQLNGIISIRELLLGLDLEILSNIMIRDVISVYADDDQQEVVQKLTNHNLSALPVTTTGNKMIGIITFDDAMDIMEEEATEDFQKLSGSEPLNESYFEASIWSIFSKRIPWLLLLFVAEAYTGTVLKYFEDEIEKVVALAFFVPLLVGTGGNTGTQVVATIIRAIGIGEVKFKDMFRVVGKEVMIGMLLGISLGIVGFIRVHILGVGSVVAQVVAITMVCIVIWSSIVAAALPLIIRKLKLDPAVISGPFITTFVDGTGLLIYFIVAKMLLNI
>NZ_CP031070.1|WP_149217189.1|190998_192459_+|HTH-domain-containing-protein
MEEITKNIIQNTTLLRKIHILEKLIENDNIIPSKILANQLHCTNRTILNDISELKQILPNNWEIITIPAKGYLLKKPNFDKLAHVITPYLINSEIHKILLGIFNCKYYTLEKWSQLLYLSKSTLKKKLENFAKTLHHFGLTFSFKTLQLTGEELNIRYFYVIYFYTIQKYKEFMNLHPYLQKKIDDITRTYGINLDINLLTIIINVFVNRHMHKCQITQNSGFKSIFYSKKVKCIKSTISEIEQFYGMNFSEKELYFFSLSYHLISEGNIKEKIDIANYFQETHREFYKASLSIFDIISSKFKLNFYAKEKIKHDIFYQINKINILKQYNLPTKYILEQCNNLKREIPELMESYGIIHPLIISWNKTLIKNRLNKNEIHYIIFNTLFAIHLNSSQKGLLLLSGPTTWKNFIYYKLNSELGNTINIYSESNNFKNYDFIITNYKIDINNIPIPVLQISDRINEQDLKYIRKFLYYNIVVKNKKINCK
>NZ_CP031070.1|WP_149217190.1|192455_193565_+|helix-turn-helix-domain-containing-protein
MKIQKSNKFRLYPTEEQAYLMRKTFGCVRFVYNRMLAERKEAYETYKGDKEQLKKQKLPTPAKYKEEFEWLKEVDSLALANAQLNLQTAYKSFFSGHNDFPTFKSKKDRKSYTTNVVNGNIMLLHGHIKLPKLKMVRIKQHREIPQEYRIKSCTISMTPTGKYYVSILTEYEKEIVQKEVETVVGLDFAMDGLYVSSEDKKANYPKFYRNMLDQLAKVQRVLSRRNKCSKRWHKQRIRVAKPHEKVANQRKNFLHHESKKSATHFDVVAIEALNMKGISQALHFGKSVADNGWGMFTSFLAYKLNEQGKQLVKIDKWFPSTKTCSNCGNVKNMTLSERVYSCICGVNLDRDYNAAINIKNEAIRLLALG
>NZ_CP031070.1|WP_149217191.1|194233_195046_+|polysaccharide-deacetylase-family-protein
MKNIRNIKRIVVTIIAIIIGCIVFKTFTSSTKSITKQEKPVQQVAEQNKIQNNKSVPQKFNGQTRKIVYLTFDDGPGKYTAKLLDILKKNQVKASFFLIGGNVTKFPDLVKQEKAEGDYIGLHSMTHDYKKLYTNGQYVKEMKEVQSLVKNVIGDSPTLTRPPYGSMPGLNEELRNKVVEEGFKVWDWTIDSLDWKYNKLPIETASNKIIQNVLSNAKHSKEIILLHDIHPQSIAAVPAIIEGLKAKGYEFEVYSEANHFPLNFWHDKRI
>NZ_CP031070.1|WP_149217192.1|195382_195787_-|phosphate-starvation-inducible-protein-PsiE
MKSFNIEFYIVNILQWILNIALVILSIILSIFLIRETIIFVKYLFTTKKYTSYELTENIMVYFLYFEFIALIIKYFKSKYHFPLRYFIYIGITAIIRLIIVSHEDPFKTLLFSGATLILVIALYISKAKGLREE

You can click texts colored in the table to view more detailed information

Click the colored protein region to show detailed information

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity
NZ_CP031070_1	1.1\|181242\|53\|NZ_CP031070\|CRT	181242-181294	53	NZ_CP031070.1	180726-180778	1	0.981
NZ_CP031070_1	1.1\|181242\|53\|NZ_CP031070\|CRT	181242-181294	53	NZ_CP031070.1	180984-181036	1	0.981

1. spacer 1.1|181242|53|NZ_CP031070|CRT matches to position: 180726-180778, mismatch: 1, identity: 0.981

ttataccagccagtttgcatttttcccattgcttctctttgttcagttgttgg	CRISPR spacer
ttataccagccagtttgcatttttcccattacttctctttgttcagttgttgg	Protospacer
******************************.**********************

2. spacer 1.1|181242|53|NZ_CP031070|CRT matches to position: 180984-181036, mismatch: 1, identity: 0.981

ttataccagccagtttgcatttttcccattgcttctctttgttcagttgttgg	CRISPR spacer
ttataccagccagtttgcatttttcccattacttctctttgttcagttgttgg	Protospacer
******************************.**********************

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP031070_1	1.1\|181242\|53\|NZ_CP031070\|CRT	181242-181294	53	NZ_CP031070	Bacillus sp. JAS24-2 plasmid pl278, complete sequence	181242-181294	0	1.0
NZ_CP031070_1	1.2\|181335\|50\|NZ_CP031070\|CRT	181335-181384	50	NZ_CP031070	Bacillus sp. JAS24-2 plasmid pl278, complete sequence	181335-181384	0	1.0
NZ_CP031070_1	1.2\|181335\|50\|NZ_CP031070\|CRT	181335-181384	50	NZ_CP031070	Bacillus sp. JAS24-2 plasmid pl278, complete sequence	181509-181558	0	1.0
NZ_CP031070_1	1.3\|181425\|44\|NZ_CP031070\|CRT	181425-181468	44	NZ_CP031070	Bacillus sp. JAS24-2 plasmid pl278, complete sequence	181425-181468	0	1.0
NZ_CP031070_1	1.4\|181509\|50\|NZ_CP031070\|CRT	181509-181558	50	NZ_CP031070	Bacillus sp. JAS24-2 plasmid pl278, complete sequence	181335-181384	0	1.0
NZ_CP031070_1	1.4\|181509\|50\|NZ_CP031070\|CRT	181509-181558	50	NZ_CP031070	Bacillus sp. JAS24-2 plasmid pl278, complete sequence	181509-181558	0	1.0
NZ_CP031070_1	1.1\|181242\|53\|NZ_CP031070\|CRT	181242-181294	53	NZ_CP031070	Bacillus sp. JAS24-2 plasmid pl278, complete sequence	180726-180778	1	0.981
NZ_CP031070_1	1.1\|181242\|53\|NZ_CP031070\|CRT	181242-181294	53	NZ_CP031070	Bacillus sp. JAS24-2 plasmid pl278, complete sequence	180984-181036	1	0.981

1. spacer 1.1|181242|53|NZ_CP031070|CRT matches to NZ_CP031070 (Bacillus sp. JAS24-2 plasmid pl278, complete sequence) position: , mismatch: 0, identity: 1.0

ttataccagccagtttgcatttttcccattgcttctctttgttcagttgttgg	CRISPR spacer
ttataccagccagtttgcatttttcccattgcttctctttgttcagttgttgg	Protospacer
*****************************************************

2. spacer 1.2|181335|50|NZ_CP031070|CRT matches to NZ_CP031070 (Bacillus sp. JAS24-2 plasmid pl278, complete sequence) position: , mismatch: 0, identity: 1.0

tgaagaattccttttgccatttctccagtttctaagcgacgatcagtagt	CRISPR spacer
tgaagaattccttttgccatttctccagtttctaagcgacgatcagtagt	Protospacer
**************************************************

3. spacer 1.2|181335|50|NZ_CP031070|CRT matches to NZ_CP031070 (Bacillus sp. JAS24-2 plasmid pl278, complete sequence) position: , mismatch: 0, identity: 1.0

tgaagaattccttttgccatttctccagtttctaagcgacgatcagtagt	CRISPR spacer
tgaagaattccttttgccatttctccagtttctaagcgacgatcagtagt	Protospacer
**************************************************

4. spacer 1.3|181425|44|NZ_CP031070|CRT matches to NZ_CP031070 (Bacillus sp. JAS24-2 plasmid pl278, complete sequence) position: , mismatch: 0, identity: 1.0

gatttccagcctgttgctatttctccgatttctaagccagtacc	CRISPR spacer
gatttccagcctgttgctatttctccgatttctaagccagtacc	Protospacer
********************************************

5. spacer 1.4|181509|50|NZ_CP031070|CRT matches to NZ_CP031070 (Bacillus sp. JAS24-2 plasmid pl278, complete sequence) position: , mismatch: 0, identity: 1.0

tgaagaattccttttgccatttctccagtttctaagcgacgatcagtagt	CRISPR spacer
tgaagaattccttttgccatttctccagtttctaagcgacgatcagtagt	Protospacer
**************************************************

6. spacer 1.4|181509|50|NZ_CP031070|CRT matches to NZ_CP031070 (Bacillus sp. JAS24-2 plasmid pl278, complete sequence) position: , mismatch: 0, identity: 1.0

tgaagaattccttttgccatttctccagtttctaagcgacgatcagtagt	CRISPR spacer
tgaagaattccttttgccatttctccagtttctaagcgacgatcagtagt	Protospacer
**************************************************

7. spacer 1.1|181242|53|NZ_CP031070|CRT matches to NZ_CP031070 (Bacillus sp. JAS24-2 plasmid pl278, complete sequence) position: , mismatch: 1, identity: 0.981

ttataccagccagtttgcatttttcccattgcttctctttgttcagttgttgg	CRISPR spacer
ttataccagccagtttgcatttttcccattacttctctttgttcagttgttgg	Protospacer
******************************.**********************

8. spacer 1.1|181242|53|NZ_CP031070|CRT matches to NZ_CP031070 (Bacillus sp. JAS24-2 plasmid pl278, complete sequence) position: , mismatch: 1, identity: 0.981

ttataccagccagtttgcatttttcccattgcttctctttgttcagttgttgg	CRISPR spacer
ttataccagccagtttgcatttttcccattacttctctttgttcagttgttgg	Protospacer
******************************.**********************

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

4542 : 91141

Bacillus_phage(33.33%)

transposase,integrase,protease,tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_149217112.1\|4542_5484_+\|transposase	IS3 family transposase	A0A1B1P773	Bacillus_phage	1.4e-38	37.4
WP_149217113.1\|7205_8324_-\|transposase	IS200/IS605 family element transposase accessory protein TnpB	S6AND0	Bacillus_phage	4.0e-173	81.2
WP_149217236.1\|9670_9991_-	hypothetical protein	NA	NA	NA	NA
WP_149217114.1\|11128_11767_-	HNH endonuclease	NA	NA	NA	NA
WP_149217115.1\|16859_17171_+	MarR family transcriptional regulator	NA	NA	NA	NA
WP_149217116.1\|17188_18637_+	cell division protein FtsZ	NA	NA	NA	NA
WP_149217117.1\|19464_20079_-	DUF3967 domain-containing protein	NA	NA	NA	NA
WP_149217118.1\|21346_21604_+	TrmB family transcriptional regulator	NA	NA	NA	NA
WP_149217119.1\|24545_24752_+	hypothetical protein	NA	NA	NA	NA
WP_149217120.1\|25203_25935_+	ATP-binding cassette domain-containing protein	G9BWD6	Planktothrix_phage	1.9e-35	39.9
WP_149217121.1\|28996_30067_-	ricin-type beta-trefoil lectin domain protein	NA	NA	NA	NA
WP_149217237.1\|30213_30987_-	hypothetical protein	NA	NA	NA	NA
WP_149217238.1\|31839_32031_+\|transposase	IS200/IS605 family element transposase accessory protein TnpB	Q332I1	Clostridium_botulinum_C_phage	5.4e-14	66.1
WP_149217122.1\|32067_32238_+\|transposase	transposase	Q332I1	Clostridium_botulinum_C_phage	2.7e-17	71.4
WP_149217123.1\|32540_33695_-	permease	NA	NA	NA	NA
WP_149217124.1\|33858_34893_-	YdcF family protein	NA	NA	NA	NA
WP_149217239.1\|37978_38212_-\|transposase	Tn3 family transposase	A0A125RQ78	Bacillus_phage	3.1e-27	82.7
WP_149217125.1\|38770_38968_+	hypothetical protein	NA	NA	NA	NA
WP_149217126.1\|42630_43002_+	DUF1093 domain-containing protein	NA	NA	NA	NA
WP_149217127.1\|43294_44056_+	ATP-binding cassette domain-containing protein	G9BWD6	Planktothrix_phage	4.5e-35	38.8
WP_149217128.1\|44072_45935_+	FtsX-like permease family protein	NA	NA	NA	NA
WP_149217129.1\|47181_47556_+	pyridoxal-phosphate dependent enzyme	NA	NA	NA	NA
WP_149217130.1\|47953_48670_-	Bax inhibitor-1/YccA family protein	NA	NA	NA	NA
WP_149217131.1\|48757_49588_-	ABC transporter permease	NA	NA	NA	NA
WP_149217132.1\|49584_50283_-	ATP-binding cassette domain-containing protein	W6JKT0	Anomala_cuprea_entomopoxvirus	1.5e-13	28.4
WP_149217133.1\|57578_58841_+\|tRNA	tyrosine--tRNA ligase	K4F5T3	Cronobacter_phage	2.5e-83	40.4
WP_149217134.1\|58871_59486_+	protein rep	NA	NA	NA	NA
WP_149217135.1\|60070_61510_+	amino acid permease	NA	NA	NA	NA
WP_149217136.1\|61566_63429_+	tyrosine decarboxylase	NA	NA	NA	NA
WP_149217137.1\|63646_64462_+	bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase	NA	NA	NA	NA
WP_149217138.1\|64697_66083_+	Na+/H+ antiporter NhaC	NA	NA	NA	NA
WP_149217139.1\|66564_66858_+	transcriptional regulator	NA	NA	NA	NA
WP_086411375.1\|69331_71158_-	group II intron reverse transcriptase/maturase	A0A0U4J920	Pseudomonas_phage	1.4e-82	35.1
WP_149217240.1\|72640_73012_-	DUF202 domain-containing protein	NA	NA	NA	NA
WP_149217140.1\|73044_73857_-	prolipoprotein diacylglyceryl transferase	NA	NA	NA	NA
WP_149217141.1\|79438_80437_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_149217142.1\|80840_82001_+\|integrase	tyrosine-type recombinase/integrase	A0A142F1N9	Bacillus_phage	6.7e-06	24.0
WP_149217143.1\|82576_83884_-	group II intron reverse transcriptase/maturase	A0A0U4J920	Pseudomonas_phage	1.0e-26	30.5
WP_001100111.1\|84057_84237_-	hypothetical protein	NA	NA	NA	NA
WP_000956453.1\|85648_85843_+	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_149217241.1\|89090_89975_+\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
WP_149217144.1\|90766_91141_-\|transposase	transposase	NA	NA	NA	NA

DBSCAN-SWA_2

158057 : 230387

Bacillus_phage(16.67%)

transposase,holin,protease

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_149217168.1\|158057_158978_-\|transposase	Rpn family recombination-promoting nuclease/putative transposase	NA	NA	NA	NA
WP_149217169.1\|160257_160713_+	MarR family transcriptional regulator	NA	NA	NA	NA
WP_149217170.1\|161255_161453_-	hypothetical protein	NA	NA	NA	NA
WP_149217171.1\|162329_162470_-\|protease	M48 family metalloprotease	NA	NA	NA	NA
WP_149217172.1\|163047_163329_+	hypothetical protein	NA	NA	NA	NA
WP_149217173.1\|163682_164306_+	DUF1361 domain-containing protein	NA	NA	NA	NA
WP_149217174.1\|164305_164599_+	hypothetical protein	NA	NA	NA	NA
WP_149217175.1\|164825_165356_-	DUF3967 domain-containing protein	A0A1Z1LZN4	Bacillus_phage	4.4e-05	33.3
WP_149217176.1\|165726_166446_+	MOSC domain-containing protein	NA	NA	NA	NA
WP_149217177.1\|166932_168144_+\|transposase	IS110 family transposase	A0A1X9I619	Streptococcus_phage	1.8e-70	38.6
WP_149217178.1\|169025_170375_-	amino acid permease	NA	NA	NA	NA
WP_149217179.1\|171004_172117_+	alanine dehydrogenase	NA	NA	NA	NA
WP_149217180.1\|172113_173115_+	bifunctional threonine ammonia-lyase/L-serine ammonia-lyase TdcB	NA	NA	NA	NA
WP_149217181.1\|173204_174596_+	amino acid permease	NA	NA	NA	NA
WP_149217182.1\|178195_179713_-	lethal factor domain protein	NA	NA	NA	NA
WP_149217183.1\|180256_183610_-	hypothetical protein	A0A1V0E026	Clostridioides_phage	1.5e-66	31.6
WP_149217184.1\|184171_184294_+\|transposase	transposase	NA	NA	NA	NA
WP_149217185.1\|184805_185417_-	sporulation membrane protein YtaF	NA	NA	NA	NA
WP_149217242.1\|185564_185696_-	N-acetylmuramoyl-L-alanine amidase	NA	NA	NA	NA
WP_149217186.1\|185692_186499_-	N-acetylmuramoyl-L-alanine amidase	A0A2H4J966	uncultured_Caudovirales_phage	1.2e-94	58.8
WP_149217187.1\|186983_187400_-\|holin	phage holin family protein	D2XPZ9	Bacillus_virus	1.3e-47	71.4
WP_149217188.1\|188785_190114_-	magnesium transporter	NA	NA	NA	NA
WP_149217189.1\|190998_192459_+	HTH domain-containing protein	NA	NA	NA	NA
WP_149217190.1\|192455_193565_+	helix-turn-helix domain-containing protein	A0A286QQB7	Streptococcus_phage	2.6e-76	45.5
WP_149217191.1\|194233_195046_+	polysaccharide deacetylase family protein	NA	NA	NA	NA
WP_149217192.1\|195382_195787_-	phosphate-starvation-inducible protein PsiE	NA	NA	NA	NA
WP_149217193.1\|196837_198181_+	Mn(2+) uptake NRAMP transporter MntH	NA	NA	NA	NA
WP_149217194.1\|199265_199979_-	response regulator	NA	NA	NA	NA
WP_149217195.1\|200086_201208_-\|transposase	IS200/IS605 family element transposase accessory protein TnpB	S6AND0	Bacillus_phage	1.7e-168	79.4
WP_149217196.1\|203092_203233_+	hypothetical protein	NA	NA	NA	NA
WP_149217197.1\|203560_204589_-	FUSC family protein	NA	NA	NA	NA
WP_149217198.1\|204848_205001_-	Fur-regulated basic protein FbpA	NA	NA	NA	NA
WP_149217199.1\|206083_206482_+\|transposase	IS3 family transposase	NA	NA	NA	NA
WP_149217243.1\|207524_207770_+	DUF418 domain-containing protein	NA	NA	NA	NA
WP_149217200.1\|208330_209014_-\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
WP_098287136.1\|210349_211657_-	group II intron reverse transcriptase/maturase	A0A0U4J920	Pseudomonas_phage	2.9e-26	30.5
WP_001100112.1\|211830_212010_-	hypothetical protein	NA	NA	NA	NA
WP_149217201.1\|212161_212374_-\|transposase	transposase	NA	NA	NA	NA
WP_149217202.1\|212837_213137_-	hypothetical protein	U5P429	Shigella_phage	1.9e-05	31.2
WP_149217203.1\|216028_216421_-	DUF1093 domain-containing protein	NA	NA	NA	NA
WP_149217204.1\|219919_220123_-	hypothetical protein	NA	NA	NA	NA
WP_149217205.1\|220383_220566_+	hypothetical protein	NA	NA	NA	NA
WP_149217206.1\|221257_222052_+	hypothetical protein	NA	NA	NA	NA
WP_149217244.1\|223000_223207_+	GTP-binding protein	NA	NA	NA	NA
WP_149217245.1\|223194_223533_+	hypothetical protein	NA	NA	NA	NA
WP_149217246.1\|223696_223939_+	GTP-binding protein	NA	NA	NA	NA
WP_149217207.1\|224083_225034_+	zinc ABC transporter solute-binding protein	NA	NA	NA	NA
WP_149217208.1\|225531_226227_+	ATP-binding cassette domain-containing protein	G3M9Y6	Bacillus_virus	1.1e-16	29.2
WP_149217247.1\|226190_227009_+	metal ABC transporter permease	NA	NA	NA	NA
WP_149217209.1\|229689_229860_+\|transposase	transposase	A0A0N9S8A3	Staphylococcus_phage	2.2e-06	42.3
WP_149217248.1\|229926_230136_+	hypothetical protein	NA	NA	NA	NA
WP_149217210.1\|230171_230387_+\|transposase	IS3 family transposase	A0A2I6AZV9	Macacine_betaherpesvirus	1.1e-10	55.1

Anti-CRISPR protein detection

Acr_ID: NZ_CP031070.1|WP_149217170.1|161255_161453_-|hypothetical-protein

Click the colored protein region to show detailed information

Acr ID

Acr position

Acr size

Homology with known anti

Neighbor HTH/AcRanker

Neighbor Aca

In prophage

Protospacer in prophage

NZ_CP031070.1|WP_149217170.1|161255_161453_-|hypothetical-protein

161255_161453_-

65 aa aa

158057-230387

yes

CRISPR ID	CRISPR type	Spacer info	Spacer location	Spacer length	Protospacer location	Mismatch
NZ_CP031070_1	TypeV	1.1\|181242\|53\|NZ_CP031070\|CRT	181242-181294	53	180726-180778	1
NZ_CP031070_1	TypeV	1.1\|181242\|53\|NZ_CP031070\|CRT	181242-181294	53	180984-181036	1

1. spacer 1.1|181242|53|NZ_CP031070|CRT matches to NZ_CP031070 position: 180726-180778, mismatch: 1

ttataccagccagtttgcatttttcccattgcttctctttgttcagttgttgg	CRISPR spacer
ttataccagccagtttgcatttttcccattacttctctttgttcagttgttgg	Protospacer
******************************.**********************

2. spacer 1.1|181242|53|NZ_CP031070|CRT matches to NZ_CP031070 position: 180984-181036, mismatch: 1

ttataccagccagtttgcatttttcccattgcttctctttgttcagttgttgg	CRISPR spacer
ttataccagccagtttgcatttttcccattacttctctttgttcagttgttgg	Protospacer
******************************.**********************

3. NZ_CP031069

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

213152 : 294301

Bacillus_phage(50.0%)

integrase,transposase,protease

attL 227485:227508|attR 289504:289527

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_149216882.1\|213152_214274_-\|transposase	IS200/IS605 family element transposase accessory protein TnpB	S6AND0	Bacillus_phage	1.1e-173	81.2
WP_149216883.1\|218271_219936_+	SpaH/EbpB family LPXTG-anchored major pilin	NA	NA	NA	NA
WP_149216884.1\|220024_220861_+	class C sortase	NA	NA	NA	NA
WP_149216885.1\|221091_222321_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_149216886.1\|222815_224045_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_149216887.1\|225633_226014_+	hypothetical protein	NA	NA	NA	NA
WP_149216888.1\|226259_227489_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
227485:227508	attL	NA	NA	NA	NA
WP_149216889.1\|227939_232691_+	DUF11 domain-containing protein	NA	NA	NA	NA
WP_149216890.1\|232720_234382_+	SpaH/EbpB family LPXTG-anchored major pilin	NA	NA	NA	NA
WP_149216891.1\|234481_235306_+	class C sortase	NA	NA	NA	NA
WP_149216892.1\|235413_236610_+	DUF4046 domain-containing protein	NA	NA	NA	NA
WP_149216893.1\|237147_237966_+	class C sortase	NA	NA	NA	NA
WP_149216894.1\|238227_239454_+	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_149216895.1\|240100_240667_-	hypothetical protein	NA	NA	NA	NA
WP_149216896.1\|240748_241039_-	metalloregulator ArsR/SmtB family transcription factor	NA	NA	NA	NA
WP_149216897.1\|242172_243168_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_149216898.1\|243345_243549_-	hypothetical protein	NA	NA	NA	NA
WP_149216899.1\|243570_244743_+\|integrase	tyrosine-type recombinase/integrase	A0A142F1N9	Bacillus_phage	3.0e-06	24.4
WP_001000186.1\|245413_245920_-	DUF664 domain-containing protein	NA	NA	NA	NA
WP_149216900.1\|246677_247103_+	DUF4879 domain-containing protein	NA	NA	NA	NA
WP_149216901.1\|247333_248125_+	ADP-ribosyltransferase	Q331X8	Clostridium_botulinum_C_phage	1.6e-27	37.7
WP_149216902.1\|249650_249863_+	small, acid-soluble spore protein, alpha/beta type	Q77YX0	Bacillus_phage	1.9e-12	56.7
WP_149217099.1\|250252_250456_-	spore germination protein	NA	NA	NA	NA
WP_149216903.1\|250563_250791_-	hypothetical protein	NA	NA	NA	NA
WP_149216904.1\|251386_252796_+	MATE family efflux transporter	NA	NA	NA	NA
WP_149216905.1\|252944_254522_-	FAD-dependent oxidoreductase	NA	NA	NA	NA
WP_149216906.1\|254877_255324_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_149216907.1\|255986_257705_+	alpha-keto acid decarboxylase family protein	A0A0P0YLY7	Yellowstone_lake_phycodnavirus	8.4e-21	24.0
WP_149216908.1\|257945_259367_+	PQQ-dependent sugar dehydrogenase	NA	NA	NA	NA
WP_149217100.1\|259693_260230_+	hypothetical protein	NA	NA	NA	NA
WP_149214957.1\|260296_261148_+	TIM barrel protein	NA	NA	NA	NA
WP_149216909.1\|261161_261959_+	hydrolase TatD	NA	NA	NA	NA
WP_149214956.1\|261973_262804_+	TIM barrel protein	NA	NA	NA	NA
WP_149216910.1\|262800_263679_+	UbiA-like protein EboC	NA	NA	NA	NA
WP_149217101.1\|263711_265034_+	alkaline phosphatase family protein	NA	NA	NA	NA
WP_149216911.1\|265038_266223_+	myo-inositol-1-phosphate synthase	NA	NA	NA	NA
WP_149216912.1\|266678_267599_-\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
WP_149216913.1\|267653_267869_-	hypothetical protein	NA	NA	NA	NA
WP_149216914.1\|268163_269750_+	amino acid permease	NA	NA	NA	NA
WP_086388367.1\|269851_270079_-	hypothetical protein	NA	NA	NA	NA
WP_149216915.1\|270815_271667_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_149216916.1\|271790_273026_+	beta-ketoacyl-ACP synthase II	NA	NA	NA	NA
WP_149216917.1\|273795_274779_+	hypothetical protein	NA	NA	NA	NA
WP_149216918.1\|274871_275774_+	hypothetical protein	NA	NA	NA	NA
WP_149216919.1\|276516_277278_+	glycosyltransferase	NA	NA	NA	NA
WP_149216920.1\|277445_277955_+	ClbS/DfsB family four-helix bundle protein	NA	NA	NA	NA
WP_149216921.1\|278658_279135_-	histidine phosphatase family protein	NA	NA	NA	NA
WP_149216922.1\|279458_281042_+	hypothetical protein	NA	NA	NA	NA
WP_149216923.1\|281978_283391_+	hypothetical protein	NA	NA	NA	NA
WP_149216924.1\|283831_284905_-	tetratricopeptide repeat protein	A0A2H4J8G3	uncultured_Caudovirales_phage	1.5e-73	42.0
WP_149216925.1\|285112_285430_+\|transposase	transposase	NA	NA	NA	NA
WP_149216926.1\|285637_286414_+	hypothetical protein	NA	NA	NA	NA
WP_149216927.1\|286613_287360_-\|protease	CPBP family intramembrane metalloprotease	NA	NA	NA	NA
WP_149216928.1\|287765_288164_-	hypothetical protein	NA	NA	NA	NA
WP_149216929.1\|288524_289508_-	glycerophosphodiester phosphodiesterase	NA	NA	NA	NA
WP_149216930.1\|289782_290694_+	ATP-binding cassette domain-containing protein	A0A2H4PQG7	Staphylococcus_phage	1.1e-35	39.0
289504:289527	attR	NA	NA	NA	NA
WP_113304807.1\|290686_291631_+	ABC transporter permease subunit	NA	NA	NA	NA
WP_149216931.1\|291826_292834_+	MBL fold metallo-hydrolase	NA	NA	NA	NA
WP_149216932.1\|293182_294301_+\|transposase	IS200/IS605 family element transposase accessory protein TnpB	S6AND0	Bacillus_phage	8.9e-173	81.5

DBSCAN-SWA_2

585858 : 593680

Bacillus_phage(90.91%)

holin

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_149217077.1\|585858_586914_-	SH3 domain-containing protein	A0A1B0T6C8	Bacillus_phage	3.1e-151	71.8
WP_149217078.1\|586910_587150_-\|holin	holin	A0A0A7AR38	Bacillus_phage	5.9e-26	74.7
WP_000377825.1\|587149_587386_-	hypothetical protein	A0A2H4J382	uncultured_Caudovirales_phage	3.0e-14	84.6
WP_149217079.1\|587455_588352_-	N-acetylmuramoyl-L-alanine amidase family protein	A0A0S2MVR5	Bacillus_phage	6.0e-79	52.1
WP_149217080.1\|588627_589470_-	peptidoglycan DD-metalloendopeptidase family protein	A0A218KCJ1	Bacillus_phage	1.0e-32	47.7
WP_149217081.1\|589592_590579_-	lysozyme family protein	A0A218KCJ1	Bacillus_phage	1.4e-33	47.5
WP_098685367.1\|590817_591204_-	hypothetical protein	A0A288WG73	Bacillus_phage	2.0e-47	71.4
WP_149217082.1\|591307_592204_-	DnaD domain protein	A0A1C8E9B4	Bacillus_phage	3.1e-75	51.2
WP_086388376.1\|592427_592664_-	helix-turn-helix transcriptional regulator	W8CYU0	Bacillus_phage	2.4e-11	58.2
WP_086388377.1\|592800_593229_+	helix-turn-helix transcriptional regulator	Q786F1	Bacillus_phage	1.6e-29	51.4
WP_065705253.1\|593251_593680_+	ImmA/IrrE family metallo-endopeptidase	Q9T201	Bacillus_phage	1.6e-34	55.1

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP031068

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NZ_CP031070

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

Click the colored protein region to show detailed information

3. NZ_CP031069

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection