CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP046735	Vibrio cholerae strain 3528-08 chromosome 2, complete sequence	0 crisprs	cas3,csx1,csa3	0	0	2	0
NZ_CP046736	Vibrio cholerae strain 3528-08 chromosome 1, complete sequence	1 crisprs	DEDDh,cas3,DinG,csx1,csa3	0	0	7	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

145078 : 154427

Escherichia_phage(33.33%)

transposase

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_001887128.1\|145078_146473_+	DEAD/DEAH box helicase	Q5GF26	Diachasmimorpha_longicaudata_entomopoxvirus	1.7e-48	36.4
WP_001887130.1\|146638_147076_+\|transposase	IS200/IS605 family transposase	A0A1P8BMC0	Lactococcus_phage	3.2e-17	34.1
WP_001887131.1\|147137_148934_-	hypothetical protein	A0A1B5FPH1	Escherichia_phage	8.2e-27	22.1
WP_001887132.1\|148933_151930_-	sensor histidine kinase	A0A1B5FPD5	Escherichia_phage	6.0e-75	32.3
WP_001915158.1\|152014_153196_-	DNA cytosine methyltransferase	M1PSQ0	Streptococcus_phage	1.2e-42	31.8
WP_001217709.1\|153383_154427_-	GMP reductase	A0A0N9Q9A5	Chrysochromulina_ericina_virus	1.8e-98	53.6

DBSCAN-SWA_2

189303 : 251137

Synechococcus_phage(22.22%)

transposase,plate

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_158106132.1\|189303_190539_+\|transposase	transposase	NA	NA	NA	NA
WP_069217048.1\|190734_191676_+	universal stress protein	NA	NA	NA	NA
WP_001889941.1\|191747_191948_+	hypothetical protein	NA	NA	NA	NA
WP_080286722.1\|192094_194770_+	DUF4040 domain-containing protein	NA	NA	NA	NA
WP_000398830.1\|194769_195123_+	Na+/H+ antiporter subunit C	NA	NA	NA	NA
WP_001887166.1\|195119_196616_+	hypothetical protein	NA	NA	NA	NA
WP_000639705.1\|196612_197089_+	Na+/H+ antiporter subunit E	NA	NA	NA	NA
WP_001883000.1\|197048_197360_+	cation:proton antiporter	NA	NA	NA	NA
WP_001887167.1\|197359_197683_+	Na+/H+ antiporter subunit G	NA	NA	NA	NA
WP_032472941.1\|197757_199089_-	ferric reductase	NA	NA	NA	NA
WP_000279461.1\|199238_199745_-	peptide deformylase	E3SLL2	Synechococcus_phage	9.0e-16	39.3
WP_158106133.1\|199887_201210_-\|transposase	IS4 family transposase	NA	NA	NA	NA
WP_158106134.1\|201333_205071_-	peptidase M66	NA	NA	NA	NA
WP_001888852.1\|205442_206654_-	L-2-hydroxyglutarate oxidase	NA	NA	NA	NA
WP_158106135.1\|206650_208792_-	TRAP transporter fused permease subunit	NA	NA	NA	NA
WP_158106136.1\|208971_209979_-	TAXI family TRAP transporter solute-binding subunit	NA	NA	NA	NA
WP_000561009.1\|210104_210215_-	hypothetical protein	NA	NA	NA	NA
WP_001888847.1\|210280_211633_-	sigma-54-dependent Fis family transcriptional regulator	NA	NA	NA	NA
WP_071170557.1\|211796_213851_+	sensor histidine kinase	A0A1V0SGX0	Hokovirus	1.8e-09	25.1
WP_080112194.1\|213964_215173_-	Spindolin	S5MR53	Choristoneura_rosaceana_nucleopolyhedrovirus	8.5e-28	34.0
WP_000720501.1\|215580_215928_+	DUF3316 domain-containing protein	NA	NA	NA	NA
WP_019829515.1\|216353_217712_+	glycerol-3-phosphate transporter	NA	NA	NA	NA
WP_000917228.1\|217865_218933_+	glycerophosphodiester phosphodiesterase	NA	NA	NA	NA
WP_001887744.1\|218967_219648_-	Crp/Fnr family transcriptional regulator	NA	NA	NA	NA
WP_001887743.1\|219787_220519_-	nucleoside phosphorylase	NA	NA	NA	NA
WP_000365210.1\|220515_221250_-	nicotinamide riboside transporter PnuC	A0A1L2CV72	Pectobacterium_phage	5.0e-07	22.1
WP_000224436.1\|221375_222380_-	LacI family DNA-binding transcriptional regulator	NA	NA	NA	NA
WP_001887742.1\|222390_223311_-	ribokinase	E3SL45	Synechococcus_phage	3.4e-05	38.6
WP_158106137.1\|223449_224334_-	ribose ABC transporter substrate-binding protein RbsB	C6ZCU4	Enterobacteria_phage	9.6e-05	23.0
WP_001887740.1\|224388_225390_-	ribose ABC transporter permease	NA	NA	NA	NA
WP_142615375.1\|225386_226889_-	ribose ABC transporter ATP-binding protein RbsA	A0A2H4PQG7	Staphylococcus_phage	1.4e-16	26.7
WP_000749918.1\|226915_227335_-	D-ribose pyranase	NA	NA	NA	NA
WP_158106138.1\|227618_227879_+	DUF3012 domain-containing protein	NA	NA	NA	NA
WP_154025657.1\|227926_228892_-	DUF1911 domain-containing protein	NA	NA	NA	NA
WP_000264537.1\|228912_230229_-	hypothetical protein	NA	NA	NA	NA
WP_158106139.1\|230231_232430_-	type VI secretion system tip protein VgrG	A0A2H4JCD4	uncultured_Caudovirales_phage	1.3e-29	24.6
WP_001882973.1\|232389_232632_-	hypothetical protein	NA	NA	NA	NA
WP_158106140.1\|232682_233948_-	type VI secretion protein	NA	NA	NA	NA
WP_001882971.1\|233995_237541_-	type VI secretion system membrane subunit TssM	NA	NA	NA	NA
WP_032469836.1\|237556_238966_-	type VI secretion system protein TssA	NA	NA	NA	NA
WP_001882968.1\|238975_239659_-	type VI secretion system-associated protein TagO	NA	NA	NA	NA
WP_000084793.1\|239628_241221_-	AAA domain-containing protein	NA	NA	NA	NA
WP_158106141.1\|241223_243833_-	type VI secretion system ATPase TssH	H6X3M6	Enterobacteria_phage	2.9e-89	29.9
WP_000083407.1\|243858_244632_-	DotU family type IV/VI secretion system protein	NA	NA	NA	NA
WP_000458007.1\|244634_245969_-\|plate	type VI secretion system baseplate subunit TssK	NA	NA	NA	NA
WP_001045311.1\|245975_246452_-	type VI secretion system lipoprotein TssJ	NA	NA	NA	NA
WP_001089708.1\|246454_247942_-	type VI secretion system-associated FHA domain protein TagH	NA	NA	NA	NA
WP_158106142.1\|247944_248961_-\|plate	type VI secretion system baseplate subunit TssG	NA	NA	NA	NA
WP_002043238.1\|248924_250694_-\|plate	type VI secretion system baseplate subunit TssF	NA	NA	NA	NA
WP_000221000.1\|250699_251137_-\|plate	type VI secretion system baseplate subunit TssE	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_CP046736

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CRISPR-Cas detection and classification

Crispr_ID: NZ_CP046736_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP046736_1

2701177-2701475

Orphan

Consensus_repeat	Method
TGCGGTCGCCGCCGCCATTGCTCGCGCTAAAGCGCGTAAAGCGGAGCAGCAGGAAACCGA	PILER-CR

2 spacers

DEDDh

The CRISPR arrays of NZ_CP046736_1

>merge|NZ_CP046736|1|2701177-2701475|PILER-CR
GCTGTCGCTGCGGCCATTGCTCGCGCTAAAGCACGTAAAGCGGAGCAGCAGGAAACCGAAAGCGCAGCTCAGCCAGCACAAGCCACGCCAAGCAGTGACGAGGCTGATCCGAAAAAAGCTGCGGTCGCCGCCGCCATTGCTCGCGCTAAAGCGCGTAAAGCGGAGCAGCAGGAAACCGAAAGCGCAGCTCAGCCAGCACAAGCCACGCCAAGCAGTGACGACGCTGACCCGAAAAAAGCTGCGGTCGCCGCCGCCATTGCTCGCGCTAAAGCGCGTAAAGCGGAGCAGCAGGAAACCGA

>NZ_CP046736|1|1|2701177-2701475|PILER-CR
GCTGTCGCTGCGGCCATTGCTCGCGCTAAAGCACGTAAAGCGGAGCAGCAGGAAACCGAA	AGCGCAGCTCAGCCAGCACAAGCCACGCCAAGCAGTGACGAGGCTGATCCGAAAAAAGCT
GCGGTCGCCGCCGCCATTGCTCGCGCTAAAGCGCGTAAAGCGGAGCAGCAGGAAACCGAA	AGCGCAGCTCAGCCAGCACAAGCCACGCCAAGCAGTGACGACGCTGACCCGAAAAAAGCT
GCGGTCGCCGCCGCCATTGCTCGCGCTAAAGCGCGTAAAGCGGAGCAGCAGGAAACCGA

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP046736.1\|WP_158106604.1\|2692087_2693383_-\|response-regulator	unknown	unknown	gnl\|CDD\|225114
NZ_CP046736.1\|WP_080561446.1\|2688505_2688988_-\|cyclic-pyranopterin-monophosphate-synthase-MoaC	unknown	unknown	gnl\|CDD\|236483
NZ_CP046736.1\|WP_019829919.1\|2708069_2709395_+\|Na+/H+-antiporter-family-protein	unknown	unknown	gnl\|CDD\|224967
NZ_CP046736.1\|WP_000141550.1\|2698241_2698823_+\|electron-transport-complex-subunit-RsxA	unknown	unknown	gnl\|CDD\|235352
NZ_CP046736.1\|WP_095490393.1\|2688995_2689508_-\|molybdenum-cofactor-biosynthesis-protein-B	unknown	unknown	gnl\|CDD\|131715
NZ_CP046736.1\|WP_001057745.1\|2704390_2705032_+\|endonuclease-III	unknown	unknown	gnl\|CDD\|182661
NZ_CP046736.1\|WP_001209531.1\|2691742_2692084_-\|phosphorelay-protein-LuxU	unknown	unknown	gnl\|CDD\|366732
NZ_CP046736.1\|WP_001045948.1\|2706136_2707021_-\|OmpA-family-protein	unknown	unknown	gnl\|CDD\|375819
NZ_CP046736.1\|WP_032472885.1\|2693813_2695844_-\|excinuclease-ABC-subunit-UvrB	unknown	unknown	gnl\|CDD\|235395
NZ_CP046736.1\|WP_158106561.1\|2701982_2703029_+\|electron-transport-complex-subunit-RsxD	unknown	unknown	gnl\|CDD\|234844
NZ_CP046736.1\|WP_001893528.1\|2707237_2707969_+\|ribonuclease-T	DEDDh	cd06127_DEDDh_CAS-I	gnl\|CDD\|179951
NZ_CP046736.1\|WP_000061070.1\|2690863_2691754_+\|YvcK-family-protein	unknown	unknown	gnl\|CDD\|211689
NZ_CP046736.1\|WP_000064969.1\|2705071_2705488_+\|lactoylglutathione-lyase	unknown	unknown	gnl\|CDD\|272886
NZ_CP046736.1\|WP_019829920.1\|2709521_2711036_-\|amidophosphoribosyltransferase	unknown	unknown	gnl\|CDD\|236427
NZ_CP046736.1\|WP_000104488.1\|2698822_2699410_+\|electron-transport-complex-subunit-RsxB	unknown	unknown	gnl\|CDD\|235347
NZ_CP046736.1\|WP_001293888.1\|2703671_2704364_+\|electron-transport-complex-subunit-E	unknown	unknown	gnl\|CDD\|237091
NZ_CP046736.1\|WP_076025639.1\|2705647_2706088_+\|DUF2753-domain-containing-protein	unknown	unknown	gnl\|CDD\|287867
NZ_CP046736.1\|WP_000954368.1\|2703040_2703670_+\|electron-transport-complex-subunit-RsxG	unknown	unknown	gnl\|CDD\|179350
NZ_CP046736.1\|WP_000598588.1\|2688263_2688509_-\|molybdopterin-synthase-sulfur-carrier-subunit	unknown	unknown	gnl\|CDD\|182985
NZ_CP046736.1\|WP_158106559.1\|2689567_2690572_-\|GTP-3',8-cyclase-MoaA	unknown	unknown	gnl\|CDD\|234672

Protein	Function_ID	Function_description	E-value
NZ_CP046736.1\|WP_158106604.1\|2692087_2693383_-\|response-regulator	gnl\|CDD\|225114	COG2204, AtoC, Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains [Signal transduction mechanisms].	6.16816e-180
NZ_CP046736.1\|WP_080561446.1\|2688505_2688988_-\|cyclic-pyranopterin-monophosphate-synthase-MoaC	gnl\|CDD\|236483	PRK09364, moaC, cyclic pyranopterin monophosphate synthase MoaC.	2.16503e-105
NZ_CP046736.1\|WP_019829919.1\|2708069_2709395_+\|Na+/H+-antiporter-family-protein	gnl\|CDD\|224967	COG2056, COG2056, Predicted permease [General function prediction only].	0
NZ_CP046736.1\|WP_000141550.1\|2698241_2698823_+\|electron-transport-complex-subunit-RsxA	gnl\|CDD\|235352	PRK05151, PRK05151, electron transport complex protein RsxA; Provisional.	4.22504e-120
NZ_CP046736.1\|WP_095490393.1\|2688995_2689508_-\|molybdenum-cofactor-biosynthesis-protein-B	gnl\|CDD\|131715	TIGR02667, Molybdenum_cofactor_biosynthesis_protein_B., molybdenum cofactor biosynthesis protein B, proteobacterial. This model represents the MoaB protein molybdopterin biosynthesis regions in Proteobacteria. This crystallized but incompletely characterized protein is thought to be involved in, though not required for, early steps in molybdopterin biosynthesis. It may bind a molybdopterin precursor. A distinctive conserved motif PCN near the C-terminus helps distinguish this clade from other homologs, including sets of proteins designated MogA. [Biosynthesis of cofactors, prosthetic groups, and carriers, Molybdopterin].	9.7999e-104
NZ_CP046736.1\|WP_001057745.1\|2704390_2705032_+\|endonuclease-III	gnl\|CDD\|182661	PRK10702, PRK10702, endonuclease III; Provisional.	2.13039e-145
NZ_CP046736.1\|WP_001209531.1\|2691742_2692084_-\|phosphorelay-protein-LuxU	gnl\|CDD\|366732	pfam01627, Hpt, Hpt domain. The histidine-containing phosphotransfer (HPt) domain is a novel protein module with an active histidine residue that mediates phosphotransfer reactions in the two-component signaling systems. A multistep phosphorelay involving the HPt domain has been suggested for these signaling pathways. The crystal structure of the HPt domain of the anaerobic sensor kinase ArcB has been determined. The domain consists of six alpha helices containing a four-helix bundle-folding. The pattern of sequence similarity of the HPt domains of ArcB and components in other signaling systems can be interpreted in light of the three-dimensional structure and supports the conclusion that the HPt domains have a common structural motif both in prokaryotes and eukaryotes. In S. cerevisiae ypd1p this domain has been shown to contain a binding surface for Ssk1p (response regulator receiver domain containing protein pfam00072).	2.89002e-08
NZ_CP046736.1\|WP_001045948.1\|2706136_2707021_-\|OmpA-family-protein	gnl\|CDD\|375819	pfam18393, MotY_N, MotY N-terminal domain. The bacterial flagellar motor is a rotary motor complex composed of various proteins. MotX and MotY are essential for the Na+-driven flagellar motor motility of Vibrio, Shewanella and Aeromonas species. MotY is main component for T-ring formation and absence of MotY completely disrupt the T-ring formation. This is the N-terminal domain of MotY which is shown to be essential for motility and responsible for the interaction with both MotX and the basal body. Functional analysis suggests that MotY-N connects the basal body to MotX and that the PomA/PomB complex associates with MotX to form the functional stator complex around the rotor. MotY-N alone does not associate strongly with the basal body, but the partial T-ring structure made of the MotY-N/MotX complex is sufficient to allow at least a few PomA/PomB stator complexes to be incorporated into the motor.	7.14257e-78
NZ_CP046736.1\|WP_032472885.1\|2693813_2695844_-\|excinuclease-ABC-subunit-UvrB	gnl\|CDD\|235395	PRK05298, PRK05298, excinuclease ABC subunit UvrB.	0
NZ_CP046736.1\|WP_158106561.1\|2701982_2703029_+\|electron-transport-complex-subunit-RsxD	gnl\|CDD\|234844	PRK00816, rnfD, electron transport complex protein RnfD; Reviewed.	0
NZ_CP046736.1\|WP_001893528.1\|2707237_2707969_+\|ribonuclease-T	gnl\|CDD\|179951	PRK05168, PRK05168, ribonuclease T; Provisional.	1.10706e-161
NZ_CP046736.1\|WP_000061070.1\|2690863_2691754_+\|YvcK-family-protein	gnl\|CDD\|211689	TIGR01826, Putative_gluconeogenesis_factor, conserved hypothetical protein, cofD-related. This model represents a subfamily of conserved hypothetical proteins that forms a sister group to the family of CofD, (TIGR01819), LPPG:Fo 2-phospho-L-lactate transferase, an enzyme of cytochrome F420 biosynthesis. Both this family and TIGR01819 are within the scope of the pfam01933. [Hypothetical proteins, Conserved].	2.20109e-151
NZ_CP046736.1\|WP_000064969.1\|2705071_2705488_+\|lactoylglutathione-lyase	gnl\|CDD\|272886	TIGR00068, Lactoylglutathione_lyase, lactoylglutathione lyase. Lactoylglutathione lyase is also known as aldoketomutase and glyoxalase I. Glyoxylase I is a homodimer in many species. In some eukaryotes, including yeasts and plants, the orthologous protein carries a tandem duplication, is twice as long, and hits this model twice. [Central intermediary metabolism, Amino sugars, Energy metabolism, Other].	1.78678e-79
NZ_CP046736.1\|WP_019829920.1\|2709521_2711036_-\|amidophosphoribosyltransferase	gnl\|CDD\|236427	PRK09246, PRK09246, amidophosphoribosyltransferase; Provisional.	0
NZ_CP046736.1\|WP_000104488.1\|2698822_2699410_+\|electron-transport-complex-subunit-RsxB	gnl\|CDD\|235347	PRK05113, PRK05113, electron transport complex protein RnfB; Provisional.	2.12863e-127
NZ_CP046736.1\|WP_001293888.1\|2703671_2704364_+\|electron-transport-complex-subunit-E	gnl\|CDD\|237091	PRK12405, PRK12405, electron transport complex RsxE subunit; Provisional.	3.98478e-134
NZ_CP046736.1\|WP_076025639.1\|2705647_2706088_+\|DUF2753-domain-containing-protein	gnl\|CDD\|287867	pfam10952, DUF2753, Protein of unknown function (DUF2753). This bacterial family of proteins has no known function.	1.22027e-94
NZ_CP046736.1\|WP_000954368.1\|2703040_2703670_+\|electron-transport-complex-subunit-RsxG	gnl\|CDD\|179350	PRK01908, PRK01908, electron transport complex protein RnfG; Validated.	2.23404e-127
NZ_CP046736.1\|WP_000598588.1\|2688263_2688509_-\|molybdopterin-synthase-sulfur-carrier-subunit	gnl\|CDD\|182985	PRK11130, moaD, molybdopterin synthase small subunit; Provisional.	7.50588e-53
NZ_CP046736.1\|WP_158106559.1\|2689567_2690572_-\|GTP-3',8-cyclase-MoaA	gnl\|CDD\|234672	PRK00164, moaA, GTP 3',8-cyclase MoaA.	0

>NZ_CP046736.1|WP_000104488.1|2698822_2699410_+|electron-transport-complex-subunit-RsxB
MSTIVIAVIALAALAAVFGAILGFASIRFKVEADPIVDQIDAILPQTQCGQCGYPGCRPYAEAIANGDAINKCPPGGQATIEKLADLMGVEVQDSAHDLDNKVKMVAFIHEDMCIGCTKCIQACPVDAIVGGNKAVHTVIKNECTGCDLCVAPCPTDCIEMIPVQTTPESWKWQLNAIPVVNVTDSAPAAQKSAN
>NZ_CP046736.1|WP_000141550.1|2698241_2698823_+|electron-transport-complex-subunit-RsxA
MTEYLLLLIGTVLVNNFVLVKFLGLCPFMGVSKKLETAIGMGLATTFVLTLASVCAYLVESYVLRPLGIEYLRTMSFILVIAVVVQFTEMVVHKTSPTLYRLLGIFLPLITTNCAVLGVALLNINENHNFIQSIIYGFGAAVGFSLVLILFASMRERIHVADVPAPFKGASIAMITAGLMSLAFMGFTGLVKL
>NZ_CP046736.1|WP_032472885.1|2693813_2695844_-|excinuclease-ABC-subunit-UvrB
MSKAFELVSDYQPAGDQPTAIKQLLEGLEAGLAHQTLLGVTGSGKTFTLANVIATAQRPTIILAPNKTLAAQLYGEMKAFFPNNAVEYFVSYYDYYQPEAYVPTTDTFIEKDASVNAHIEQMRLSATKALLERKDVVIVASVSAIYGLGDPDSYLKMMLHLRRGDVINQRDMLRRLAELQYSRNDMAFERGQFRVRGEVIDIFPAESDQEAVRVEMFDDEVECISLFDPLTGVIMSRDLARFTIYPKTHYVTPRERILEAIEQIKSELQVRRQYLLDNNKLLEEQRISQRTQFDIEMMNELGFCSGIENYSRYLSGRAEGEPPPTLFDYLPHDGLLIIDESHVTVPQIGAMFKGDRSRKETLVEFGFRLPSALDNRPLKFDEFEALAPQTIFVSATPSEYELTKSGGEVAEQVVRPTGLLDPIIEVRPVATQVDDLLSEARIRAANDERILVTTLTKRMAEDLTEYLHEHGVKVRYLHSDIDTVERVEIIRDLRLGVFDVLVGINLLREGLDMPEVALVAILDADKEGFLRSERSLIQTMGRAARNVNGKAILYADSITKSMRKAIDETERRREKQLAYNEQRGITPQPLKRSVKDIMELGDIAKSRKQKNSKVVPLAKVAEESAAYQVLTPQQLEKEISKLEAAMYQHAQNLEFELAAQKRDEIHQLRQQFIANS
>NZ_CP046736.1|WP_158106604.1|2692087_2693383_-|response-regulator
MVEDTASVAALYRSYLTPLDIDINIVGTGRDAIESIGRREPDLILLDLRLPDMTGMDVLYAVKEKSPDVPIVFMTAHGSIDTAVEAMRHGAQDFLIKPCEADRLYQGFIGSSQTMQAVYRTIDSAASSKASIFITGESGTGKEVCAEAIHAASKRGDKPFIAINCAAIPKDLIESELFGHVKGAFTGAATERQGAAEAADGGTLFLDELCEMDLDLQTKLLRFIQTGTFQKVGSSKMKSVDVRFVCATNRDPWKEVQEGRFREDLYYRLYVIPLHLPPLRARGDDVIEIAYSLLGFMSKEEGKDFVRLSAEVVERFRQYEWPGNVRQLQNVLRNVVVLNEGREITLDMLPPPLNQMYAPINRALPLAHENKVSVHEIFPLWMTEKQAIEQAIEACDGNIPRAATYLDVSPSTIYRKLQTWNEKVKEKEKER
>NZ_CP046736.1|WP_001209531.1|2691742_2692084_-|phosphorelay-protein-LuxU
MREWINQSKIDLLAKEIGEENVPILVNIFLGELNDYQSKLVSDTVADKLGYLKEISHALKSSAASFGADRLCAKAVELDSRAKSGEMMDISLEVEHMLELLKQTHQCYSDLVH
>NZ_CP046736.1|WP_000061070.1|2690863_2691754_+|YvcK-family-protein
MSLYENKKVVAIGGGHGLGRMLAALKVFGSNATGIVTTTDNGGSTGRIRHCQGGIAWGDTRNCINQLITEPSISSMMFEYRFKGAGELNGHNLGNLMLTALDNLSVRPLDAINLIRNMLKVDVNILPMSEHPSDLAALAMDGKWVTGETSVDEMTQDLRRLDLAPEVPATKEAVTAIQDADCVILGPGSFLTSVMPPLLLPELGKAIARNQTAKVIFVENLSPEYGPAGRMSLEQKLEWCERACQGRKIDVVLGHHPHPELEQRWNFVTRDLASANRDWRHDRQKLRQAIEEQLMN
>NZ_CP046736.1|WP_158106559.1|2689567_2690572_-|GTP-3',8-cyclase-MoaA
MERCSVAQQFEDRFQRKFYYLRLSITDVCNFKCTYCLPDGYKLSADRPASFLTVNEIRRVVSAFAHCGTSKVRITGGEPSLRKDFGEIIHAIAQTPGIQKVATTTNGYRLAKHIGEWREAGLTQLNVSVDSLDPRMFAQITGENRFQQVMSGIDRAFEVGFEQVKVNVVLMKNLNHLELPKFMAWIKTRPIQLRFIELMQTGEMDALFARHHVSGISIRDYLFGNGWLLKARADNDGPAQVFIHPDFQGEIGLIMPYEKDFCRSCNRLRVSALGKLHLCLFGEQGIELRDLLQDDHQENALIERIQTQLQNKAETHFLHDGNSGMTPHLASIGG
>NZ_CP046736.1|WP_095490393.1|2688995_2689508_-|molybdenum-cofactor-biosynthesis-protein-B
MGHAESKFQAANIAVLTVSDTRTEENDTSGRYLAEQLQQAGHTLADKQIVIDDMYKIRAVVSQWIADETIQAILITGGTGFTSRDSTPEALKPLFDKEVEGFGELFRMVSFEEIGTSTIQSRAVAGFANHTVIFAMPGSTGACRTGWTKIIKQQLDASHRPCNFMPHLTV
>NZ_CP046736.1|WP_080561446.1|2688505_2688988_-|cyclic-pyranopterin-monophosphate-synthase-MoaC
MMSQLTHINASGEANMVDVSNKADTVREARAEAYVRMAPETLQLILSGQHHKGDVFATARIAGIQAAKRTWELIPLCHPLLLSKVEVQLEALLEQNSVRIESLCKLSGKTGVEMEALTAASVAALTIYDMCKAVQKDIVIENVRLLEKSGGKSGHFKVDA
>NZ_CP046736.1|WP_000598588.1|2688263_2688509_-|molybdopterin-synthase-sulfur-carrier-subunit
MIKVLFFAQTRELVECDSLVVDHAFATVEALRQHLAQQPGKWDIALEPGKLLAAVNQSIVPFDTELQDGDEVAFFPPVTGG
>NZ_CP046736.1|WP_158106561.1|2701982_2703029_+|electron-transport-complex-subunit-RsxD
MAFFIASSPHLRSKRSTADVMRWVLACALPGLIAQTYFFGYGTLIQLLLAISVAVALEAGIMLLRKRSPISALRDYSAVVTAWLLAVAIPPLSPWWVVVIGLIFAIVIAKHLYGGLGQNPFNPAMIAYVVLLISFPVQMTSWMAPIKLTAEPSSLVDSFSLIFGGFDSDGLSLQQIRTGIDGITMATPLDAIKTSLKAGHTMSETLIQPQFSGFAGIGWEWVNIAYLLGGLILLKLRIIRWHIPMAMLAGLVFTALLAQLFAPGTTASPMIHLLSGATMLGAFFIATDPVSASTTDKGRLIYGFFIGAMVFLIRSWGGFPDGVAFAVLLANMCVPLIDYYTKPRTYGH
>NZ_CP046736.1|WP_000954368.1|2703040_2703670_+|electron-transport-complex-subunit-RsxG
MLTAIRKNGLILAVFACVSTGLVALTYALTAEQIQQQEQKQLLQVLNQVIPHKYHDNPLAQACTLVNDDKLGTAKTMHAYLAQRDGQPTAIAIETIAPDGYNGEIKLIVGIANNGTVLGVRVLAHQETPGLGDKIDLRISNWVLGFNGQQVTADNQDDWKVRKDGGQFDQFTGATITPRAVVLAVKKAVEYVNQHQQQLHNQPNPCEGQ
>NZ_CP046736.1|WP_001293888.1|2703671_2704364_+|electron-transport-complex-subunit-E
MSENRTLMLNGMWNNNPALVQLLGLCPLLAVSSTVTNALGLGIATLLVLVGSNVTVSLVRDYVPKEVRIPVFVMIIASLVTCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDVLPAALDGFWMGLGMTSVLVVLGSLREIIGNGTLFDGADLLLGEWAKVLRIEVFHFDSAFLLALLPPGAFIGVGFLIAAKSVIDKQIAARQPKQQKQAIERARVTNV
>NZ_CP046736.1|WP_001057745.1|2704390_2705032_+|endonuclease-III
MNNAKRIEILERLRANNPKPETELNWNSPFELLIAVLLSAQATDVSVNKATDKLYAVANTPQAMLDLGVDGVKEYIKTIGLFNSKAENVIKTCRILLEKHQGEVPEDREALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVSNRTKFAVGKNVDEVEHKLLKVVPNEFKLDVHHWLILHGRYTCVARKPRCGSCIIEDLCEFKDKVYPES
>NZ_CP046736.1|WP_000064969.1|2705071_2705488_+|lactoylglutathione-lyase
MSNHRILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIGVNDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQNKQAHAGLEG
>NZ_CP046736.1|WP_076025639.1|2705647_2706088_+|DUF2753-domain-containing-protein
MMNIKEWERHTLLADIAMQESDHLRSILHYQQALTVSQQLDESEEIDMEDRMMISVISCHNMAKFWRTIGDNQYELKYLQLASERVLTLIPQCHKPGCEAFVDSLGCCRKALLDFMKRHPNPEIARMVQHIDTASKCEIIAQFRLN
>NZ_CP046736.1|WP_001045948.1|2706136_2707021_-|OmpA-family-protein
MNKWVITSLFAISTLSASVASALEVRYVATPQQSTWQMATNTPLECRLVHPIPNYGDAEFSSRAGKKINLDFELKMRRPMGDTRNVSLVSMPPVWRPGEGADRITNLQFFKQFDGYIGGQTAWSLLSELEKGRYPTFSYQDWQSRDQRIEVALSSVLFQQPYNEFSDCISKLLPYSFEDISFTILHYDQGTSVELNKASQKRLAQIAEYVRYNQDIDLVLVSTYTDSTDTNNNSQNLSEQRAEVLREYFKSIGLPEDRIQVQGYGKRRPIADNASPIGKDKNRRVVISLGRTQV
>NZ_CP046736.1|WP_001893528.1|2707237_2707969_+|ribonuclease-T
MLHRRICNNASLITQFRTRMTDKNDALTLKKRFRGYFPVVIDVETAGFNAQTDALLEICAVTLSMDENGDLHPASTIHFHVEPFEGANLEKAALEFTGIYDPFSPLRGAVSEDHALKEIYKLVRKEQKAADCSRAIIVAHNAHFDHSFVMAASERCKLKRVPFHPFATFDTATLSGLAFGQTVLAKACKTAGMEFDNREAHSALYDTQKTAELFCTIVNQWKALGGWPLVNDDEDNNNDADLD
>NZ_CP046736.1|WP_019829919.1|2708069_2709395_+|Na+/H+-antiporter-family-protein
MNPVVISVCVMLLLALMRVNVVVALTFSAVVGGLVAGMSLNETVAAFESGLGGGATIALSYAMLGTFAVAISKSGITDLLAQSVIRRIHGQESHAASTGLKYLVLISLLLMAMASQNVIPVHIAFIPILIPPLLSVFAKLKLDRRLVACVLTFGLITPYMVLPVGFGGIFLNNILLKNLHENGLESITASQVPTAMILPAAGMIFGLLLAVLFSYRKPREYQEMELTVVQQESSSINKQHIAIAGVGIVAALAVQLLTGSMIIGALAGFMVFTFGGVIAWKETHDVFTRGVHMMAMIGFIMISAAGFAAVMKATGGVETLVTSLSTSIGDNKALAALLMLVVGLLVTMGIGSSFSTIPIIATIYVPLCLSFGFSPMATVALVGTAAALGDAGSPASDSTLGPTSGLNADGQHEHIWETVVPTFIHYNLPLIVFGWIAAMVL
>NZ_CP046736.1|WP_019829920.1|2709521_2711036_-|amidophosphoribosyltransferase
MCGIVGIVGTTPVNQSIYDALTVLQHRGQDAAGICTIESNRFRLRKANGLVRDVFEAKHMQRLQGNVGIGHVRYPTAGSSSASEAQPFYVNSPFGITLAHNGNLTNANQVRQKLFEKDRRHVNTTSDSEVLLNVLAHEIDTVKGNVTAEDVFRAISNVHRTIRGAYAVAAMIIGHGMIAFRDPHGIRPLCLGKREVNGHLEYMVASESVALDAVGFDFVRDVAPGEAIYATFDGELYTKQCADNPALNPCIFEFVYFARPDSFIDKISVYSARVEMGKRLGERIKNDYSDLDIDVVIPIPETSCDIALQIAQAIDKPYRQGFVKNRYVGRTFIMPGQQQRKKSVRRKLNAIRSEFKDKNVLLVDDSIVRGTTSEQIIEMARDSGAKKVYIVSAAPEIRFPNVYGIDMPSANELIAHGRDNDAICKQIGADALIFQTLEDLVEAVRCGNPDIVKFEASVFNGEYVTGDIDQQYLDFLESMRSDDAKVQREIQQDLANLELHNEGA

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Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

124075 : 131339

uncultured_Mediterranean_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_001888660.1\|124075_124963_+	cysteine synthase CysM	A0A1X9I5K7	Streptococcus_phage	3.2e-56	41.1
WP_158106282.1\|125230_127819_+	DNA mismatch repair protein MutS	A0A1V0SC35	Catovirus	1.7e-33	23.0
WP_000116735.1\|127912_128920_-	RNA polymerase sigma factor RpoS	F4YCU2	Synechococcus_phage	6.0e-35	34.2
WP_000177561.1\|128993_129929_-	murein hydrolase activator NlpD	D7RWE0	Brochothrix_phage	7.8e-05	34.7
WP_000002982.1\|129928_130555_-	protein-L-isoaspartate(D-aspartate) O-methyltransferase	A0A1B1IU40	uncultured_Mediterranean_phage	2.0e-36	52.1
WP_001888657.1\|130547_131339_-	5'/3'-nucleotidase SurE	A0A1B1ITZ2	uncultured_Mediterranean_phage	5.0e-69	52.0

DBSCAN-SWA_2

426785 : 434981

Micromonas_sp._RCC1109_virus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_001887557.1\|426785_428405_-	aldolase	E5EQ69	Micromonas_sp._RCC1109_virus	1.1e-11	27.5
WP_001887558.1\|428397_430218_-	ABC transporter ATP-binding protein	W8CYL7	Bacillus_phage	3.1e-26	24.5
WP_001887559.1\|430226_431369_-	DegT/DnrJ/EryC1/StrS family aminotransferase	E5ES42	Bathycoccus_sp._RCC1105_virus	8.5e-38	29.2
WP_050501597.1\|431365_432553_-	formyl transferase	A0A220T679	Eptesipox_virus	2.0e-05	41.9
WP_032472847.1\|432524_432947_-	WxcM-like domain-containing protein	NA	NA	NA	NA
WP_019827646.1\|432943_433825_-	glucose-1-phosphate thymidylyltransferase RfbA	I7I009	Enterobacteria_phage	4.0e-112	68.1
WP_000699461.1\|433907_434981_-	dTDP-glucose 4,6-dehydratase	A0A1D7XFE8	Escherichia_phage	7.6e-97	51.2

DBSCAN-SWA_3

1246048 : 1252660

Staphylococcus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_000210573.1\|1246048_1247182_+	glutamate 5-kinase	A0A1X9I5D0	Streptococcus_phage	1.4e-64	40.4
WP_001893543.1\|1247190_1248444_+	glutamate-5-semialdehyde dehydrogenase	A0A1X9I5D4	Streptococcus_phage	3.9e-100	49.5
WP_000543544.1\|1248543_1248993_+	transcriptional regulator NrdR	NA	NA	NA	NA
WP_001888607.1\|1249018_1250122_+	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	3.5e-44	33.3
WP_000493874.1\|1250126_1250780_+	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	1.2e-31	38.2
WP_001122865.1\|1250820_1251930_+	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	6.7e-64	39.8
WP_000864130.1\|1252189_1252660_+	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	6.8e-34	48.7

DBSCAN-SWA_4

1745778 : 1830581

100

Vibrio_phage(89.13%)

portal,transposase,integrase,lysis,head,capsid,protease,tail,terminase

attL 1739558:1739574|attR 1807000:1807016

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
1739558:1739574	attL	NA	NA	NA	NA
WP_001886870.1\|1745778_1750302_+\|protease	SslE/AcfD family lipoprotein zinc metalloprotease	NA	NA	NA	NA
WP_001886869.1\|1751179_1752454_-	hypothetical protein	NA	NA	NA	NA
WP_001013534.1\|1752557_1753370_-	solute-binding protein	NA	NA	NA	NA
WP_001886868.1\|1753609_1754443_+	hypothetical protein	NA	NA	NA	NA
WP_158106420.1\|1754429_1754633_+	hypothetical protein	NA	NA	NA	NA
WP_001886867.1\|1754798_1756391_-	hypothetical protein	NA	NA	NA	NA
WP_032472471.1\|1756689_1757331_+	hypothetical protein	NA	NA	NA	NA
WP_032472472.1\|1757472_1757727_+	hypothetical protein	NA	NA	NA	NA
WP_000734971.1\|1757728_1758262_+	type III secretion protein	NA	NA	NA	NA
WP_000830400.1\|1758263_1758722_+	hypothetical protein	NA	NA	NA	NA
WP_001001657.1\|1758751_1759240_+	hypothetical protein	NA	NA	NA	NA
WP_001886865.1\|1759252_1760620_+	hypothetical protein	NA	NA	NA	NA
WP_001886864.1\|1760620_1761766_+	hypothetical protein	NA	NA	NA	NA
WP_000858304.1\|1762122_1763172_+	hypothetical protein	NA	NA	NA	NA
WP_000026208.1\|1763182_1763908_+	hypothetical protein	NA	NA	NA	NA
WP_001246761.1\|1763952_1764555_+	hypothetical protein	NA	NA	NA	NA
WP_032467989.1\|1764481_1764889_+	hypothetical protein	NA	NA	NA	NA
WP_032472473.1\|1764899_1765475_+	hypothetical protein	NA	NA	NA	NA
WP_158106421.1\|1765772_1769759_+	hypothetical protein	NA	NA	NA	NA
WP_088131832.1\|1769871_1770483_+	hypothetical protein	NA	NA	NA	NA
WP_001886860.1\|1770479_1772363_+	type III secretion protein	NA	NA	NA	NA
WP_001886859.1\|1772383_1773436_+	EscU/YscU/HrcU family type III secretion system export apparatus switch protein	NA	NA	NA	NA
WP_001886858.1\|1773410_1774217_+	OmpA family protein	NA	NA	NA	NA
WP_000842251.1\|1774206_1774599_+	VPA1352 family putative T3SS effector	NA	NA	NA	NA
WP_158106422.1\|1774576_1775590_+	VPA1351 family putative T3SS effector	NA	NA	NA	NA
WP_095461408.1\|1775595_1776249_+	VPA1350 family putative T3SS effector	NA	NA	NA	NA
WP_000861356.1\|1776263_1776479_+	FliM/FliN family flagellar motor switch protein	NA	NA	NA	NA
WP_158106423.1\|1776691_1777243_+	transcriptional regulator	NA	NA	NA	NA
WP_139284602.1\|1778095_1778395_+	hypothetical protein	NA	NA	NA	NA
WP_001910588.1\|1778443_1779130_+	EscR/YscR/HrcR family type III secretion system export apparatus protein	NA	NA	NA	NA
WP_001186128.1\|1779132_1779816_+	hypothetical protein	NA	NA	NA	NA
WP_000181845.1\|1779808_1780057_+	VPA1340 family putative T3SS effector	NA	NA	NA	NA
WP_002052990.1\|1780046_1781504_+	vcsC2	G4WZN6	Enterobacteria_phage	1.3e-06	23.3
WP_000922351.1\|1781503_1782766_+	hypothetical protein	NA	NA	NA	NA
WP_088136745.1\|1782845_1783115_+	VPA1337 family putative T3SS effector	NA	NA	NA	NA
WP_001886853.1\|1783107_1783848_+	hypothetical protein	NA	NA	NA	NA
WP_000217070.1\|1783834_1784068_+	flagellar biosynthetic protein FliQ	NA	NA	NA	NA
WP_000854879.1\|1784082_1784457_+	hypothetical protein	NA	NA	NA	NA
WP_000836879.1\|1784457_1784943_-	hypothetical protein	NA	NA	NA	NA
WP_001886852.1\|1784926_1785694_-	transcriptional regulator	NA	NA	NA	NA
WP_019829802.1\|1785932_1786691_+	hypothetical protein	NA	NA	NA	NA
WP_000472892.1\|1786731_1787013_+	hypothetical protein	NA	NA	NA	NA
WP_001886849.1\|1787245_1787860_+	hypothetical protein	NA	NA	NA	NA
WP_032472476.1\|1788067_1788721_+	AcfA family outer membrane beta-barrel protein	NA	NA	NA	NA
WP_001886848.1\|1789609_1790845_-\|integrase	tyrosine-type recombinase/integrase	A0A1V0E8G8	Vibrio_phage	1.0e-145	61.4
WP_001886847.1\|1791446_1792853_-\|tail	tail fiber domain-containing protein	A0A1E1GDN9	Vibrio_phage	2.4e-13	35.2
WP_158106424.1\|1792852_1794562_-	hypothetical protein	NA	NA	NA	NA
WP_032472478.1\|1794561_1794843_-	hypothetical protein	A0A1V0E868	Vibrio_phage	1.3e-24	60.4
WP_154388724.1\|1794907_1795069_-	hypothetical protein	NA	NA	NA	NA
WP_139284600.1\|1795079_1796492_-	hypothetical protein	A0A2I7R7L5	Vibrio_phage	3.9e-40	36.7
WP_158106425.1\|1796472_1796637_-	hypothetical protein	A0A1V0E896	Vibrio_phage	3.8e-08	50.9
WP_158106426.1\|1796645_1800536_-	DUF1983 domain-containing protein	A0A1V0E886	Vibrio_phage	0.0e+00	79.5
WP_148506517.1\|1800547_1801156_-\|tail	tail assembly protein	A0A1V0E8A0	Vibrio_phage	4.1e-71	70.8
WP_158106427.1\|1801225_1801642_-	hypothetical protein	NA	NA	NA	NA
WP_158106428.1\|1801974_1802802_+	hypothetical protein	I6R977	Salmonella_phage	2.7e-33	40.6
WP_158106429.1\|1802923_1803964_+\|transposase	IS481 family transposase	A0A077SLK2	Escherichia_phage	5.0e-170	81.8
WP_158106430.1\|1804060_1804843_-	peptidase P60	A0A1V0E882	Vibrio_phage	2.0e-78	54.3
WP_158106431.1\|1804844_1805597_-\|tail	phage minor tail protein L	A0A1V0E876	Vibrio_phage	5.7e-115	78.2
WP_032472482.1\|1805600_1805939_-\|tail	phage tail protein	A0A1V0E890	Vibrio_phage	2.3e-47	75.0
WP_158106432.1\|1805941_1808758_-\|tail	phage tail tape measure protein	A0A1V0E821	Vibrio_phage	3.8e-289	58.7
1807000:1807016	attR	NA	NA	NA	NA
WP_158003290.1\|1808769_1808913_-	hypothetical protein	NA	NA	NA	NA
WP_158106433.1\|1808942_1809458_-	hypothetical protein	A0A1V0E823	Vibrio_phage	1.0e-43	70.0
WP_158106434.1\|1809469_1809949_-\|tail	phage tail protein	A0A1U9GN34	Vibrio_phage	2.5e-76	89.8
WP_032472484.1\|1810029_1810398_-	DUF3168 domain-containing protein	A0A1V0E8A7	Vibrio_phage	2.6e-52	75.4
WP_158106435.1\|1810394_1810862_-	hypothetical protein	A0A1V0E895	Vibrio_phage	1.8e-55	82.7
WP_158106436.1\|1810864_1811227_-\|head,tail	head-tail adaptor protein	A0A1V0E8B6	Vibrio_phage	1.5e-44	67.2
WP_158106437.1\|1811226_1811520_-\|head,tail	phage gp6-like head-tail connector protein	A0A1V0E887	Vibrio_phage	1.4e-32	68.6
WP_158106438.1\|1811534_1811804_-	hypothetical protein	A0A1V0E889	Vibrio_phage	2.2e-08	54.1
WP_071181049.1\|1811876_1813064_-\|capsid	phage major capsid protein	A0A1V0E898	Vibrio_phage	1.4e-168	79.2
WP_080482709.1\|1813068_1813947_-\|protease	Clp protease ClpP	A0A1V0E8B8	Vibrio_phage	3.2e-133	81.8
WP_001886835.1\|1813949_1815257_-\|portal	phage portal protein	A0A1V0E8B9	Vibrio_phage	5.4e-214	83.0
WP_032472489.1\|1815256_1815457_-	hypothetical protein	NA	NA	NA	NA
WP_001886832.1\|1815456_1817172_-\|terminase	terminase large subunit	A0A1V0E8C2	Vibrio_phage	0.0e+00	91.8
WP_032472490.1\|1817168_1817642_-\|terminase	phage terminase small subunit P27 family	A0A1V0E8B7	Vibrio_phage	4.9e-64	73.9
WP_032472491.1\|1817647_1818007_-	HNH endonuclease	A0A1V0E8A5	Vibrio_phage	1.2e-51	79.8
WP_139284597.1\|1818010_1818331_-	hypothetical protein	A0A1V0E8K3	Vibrio_phage	1.8e-22	55.7
WP_032472493.1\|1818394_1818601_-	hypothetical protein	A0A1V0E8A3	Vibrio_phage	2.4e-20	69.7
WP_158106439.1\|1819448_1819889_-\|lysis	lysis protein	A0A1V1FDS3	Vibrio_phage	3.1e-20	47.7
WP_080285581.1\|1819860_1820472_-	lysozyme	A0A1V0E844	Vibrio_phage	1.3e-85	79.5
WP_044126697.1\|1820458_1820644_-	hypothetical protein	A0A1V0E826	Vibrio_phage	2.4e-11	55.7
WP_044126696.1\|1820775_1821444_-	hypothetical protein	A0A1V0E841	Vibrio_phage	4.0e-72	60.3
WP_158106440.1\|1821506_1821653_-	hypothetical protein	NA	NA	NA	NA
WP_148529107.1\|1821672_1822056_-	hypothetical protein	NA	NA	NA	NA
WP_158106441.1\|1822066_1823080_-	DUF968 domain-containing protein	A0A1U9GXE8	Vibrio_phage	4.8e-133	67.5
WP_044126693.1\|1823073_1823358_-	DUF4406 domain-containing protein	A0A1U9GY09	Vibrio_phage	2.9e-40	79.6
WP_044126692.1\|1823354_1823618_-	hypothetical protein	NA	NA	NA	NA
WP_158106442.1\|1823614_1824121_-	hypothetical protein	A0A1V1FCN0	Vibrio_phage	9.9e-63	72.1
WP_158106443.1\|1824117_1824423_-	hypothetical protein	NA	NA	NA	NA
WP_158106444.1\|1824415_1824853_-	hypothetical protein	A0A2I7S3S8	Vibrio_phage	4.9e-10	32.7
WP_158106445.1\|1824858_1825047_-	hypothetical protein	A0A1V0E836	Vibrio_phage	2.0e-08	48.3
WP_158106446.1\|1825043_1825250_-	hypothetical protein	NA	NA	NA	NA
WP_071181037.1\|1825422_1826121_-	DNA-binding protein	A0A1V0E838	Vibrio_phage	8.8e-70	61.0
WP_052382155.1\|1826121_1826304_-	hypothetical protein	A0A1V0E856	Vibrio_phage	4.0e-06	40.0
WP_158106447.1\|1826315_1827305_-	ATPase	R9TPU9	Vibrio_phage	1.0e-42	57.4
WP_032472503.1\|1827297_1827570_-	hypothetical protein	NA	NA	NA	NA
WP_071181035.1\|1827574_1828228_-	phage N-6-adenine-methyltransferase	Q8HA94	Salmonella_phage	6.8e-48	47.6
WP_071181034.1\|1828411_1828618_-	hypothetical protein	A0A1V0E8D3	Vibrio_phage	2.0e-14	66.2
WP_032472504.1\|1828614_1829067_-	hypothetical protein	R9TRN6	Vibrio_phage	2.9e-42	63.0
WP_080482707.1\|1829361_1830156_+	helix-turn-helix transcriptional regulator	R9TNM0	Vibrio_phage	1.4e-76	64.5
WP_071181033.1\|1830182_1830581_+	hypothetical protein	A0A0P0IYG9	Acinetobacter_phage	4.3e-05	32.3

DBSCAN-SWA_5

1834111 : 1846437

Vibrio_phage(83.33%)

integrase

attL 1831166:1831181|attR 1855105:1855120

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
1831166:1831181	attL	NA	NA	NA	NA
WP_071181029.1\|1834111_1835221_+	recombination-associated protein RdgC	A0A2I7RNT5	Vibrio_phage	2.8e-86	54.3
WP_158106449.1\|1835271_1835862_+	hypothetical protein	K7R9L4	Vibrio_phage	4.4e-70	57.9
WP_158106450.1\|1835858_1837376_+	DNA cytosine methyltransferase	A0A1L5C2B8	Pseudoalteromonas_phage	1.3e-153	52.2
WP_158106451.1\|1837386_1837860_+	hypothetical protein	NA	NA	NA	NA
WP_158106452.1\|1837843_1838335_+	hypothetical protein	A0A1V0E8E0	Vibrio_phage	3.4e-44	55.0
WP_158106453.1\|1838364_1838574_+	hypothetical protein	A0A1V0E8D5	Vibrio_phage	2.5e-20	63.8
WP_158106454.1\|1838582_1838843_+	hypothetical protein	A0A1V0E8E7	Vibrio_phage	6.3e-13	53.8
WP_158106455.1\|1838866_1839094_+	hypothetical protein	A0A2H4JB60	uncultured_Caudovirales_phage	7.6e-15	58.4
WP_158106456.1\|1839103_1839940_+	HNH endonuclease	A0A1S5SAI9	Streptococcus_phage	4.2e-34	39.8
WP_158106457.1\|1839884_1840382_+	hypothetical protein	A0A1V0E8C4	Vibrio_phage	6.3e-70	77.5
WP_032472514.1\|1840374_1840626_+	hypothetical protein	A0A1V0E8C3	Vibrio_phage	1.6e-18	61.0
WP_158106458.1\|1840646_1841282_+	hypothetical protein	A0A1V0E8E6	Vibrio_phage	3.6e-78	64.1
WP_158106459.1\|1841294_1841642_+	hypothetical protein	NA	NA	NA	NA
WP_158106460.1\|1841638_1842280_+	hypothetical protein	A0A059WLH8	Vibrio_phage	5.1e-72	63.1
WP_032472518.1\|1842276_1842504_+	hypothetical protein	A0A1V0E8F5	Vibrio_phage	2.9e-22	67.6
WP_152554674.1\|1842607_1842850_+	hypothetical protein	A0A067ZIB4	Vibrio_phage	3.6e-31	82.2
WP_158106461.1\|1842855_1843125_+	hypothetical protein	NA	NA	NA	NA
WP_032472521.1\|1843124_1843559_+	hypothetical protein	A0A2I7RS33	Vibrio_phage	6.1e-13	36.2
WP_043881223.1\|1843954_1844287_+	hypothetical protein	A0A1V0E8F8	Vibrio_phage	8.2e-26	66.7
WP_158106462.1\|1844298_1844517_+	hypothetical protein	NA	NA	NA	NA
WP_032472524.1\|1844521_1844761_+	hypothetical protein	NA	NA	NA	NA
WP_032472525.1\|1844757_1844991_+	hypothetical protein	NA	NA	NA	NA
WP_032472526.1\|1844992_1845190_+	AlpA family transcriptional regulator	A0A1V0E8E5	Vibrio_phage	2.9e-18	70.8
WP_001886817.1\|1845186_1846437_-\|integrase	tyrosine-type recombinase/integrase	A0A1V0E8G8	Vibrio_phage	8.3e-204	82.9
1855105:1855120	attR	NA	NA	NA	NA

DBSCAN-SWA_6

2073432 : 2130607

Vibrio_phage(81.63%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_158106482.1\|2073432_2087109_+	MARTX multifunctional-autoprocessing repeats-in-toxin holotoxin RtxA	B3Y8K3	Vibrio_virus	8.4e-07	100.0
WP_000593519.1\|2087567_2087942_-	cholera enterotoxin binding subunit CtxB	A0A142I6Y1	Vibrio_phage	5.9e-65	100.0
WP_001888508.1\|2087938_2088715_-	cholera enterotoxin catalytic subunit CtxA	A0A023W6A1	Vibrio_virus	7.8e-152	100.0
WP_000979342.1\|2090011_2090305_-	accessory cholera enterotoxin	A0A0F6YNQ7	Vibrio_phage	3.7e-46	100.0
WP_080482798.1\|2090301_2091582_-	hypothetical protein	A0A142I701	Vibrio_phage	3.3e-216	93.4
WP_000493022.1\|2091592_2091841_-	colonization factor	A0A0N7F140	Vibrio_phage	8.8e-33	100.0
WP_001888513.1\|2091976_2092357_-	hypothetical protein	A0A0N9HJ67	Vibrio_phage	4.0e-69	100.0
WP_019830347.1\|2092334_2093414_-	hypothetical protein	A0A023W5Z8	Vibrio_virus	2.2e-213	100.0
WP_000492997.1\|2093545_2093881_+	helix-turn-helix transcriptional regulator	A0A0N9HJE7	Vibrio_phage	4.7e-53	100.0
WP_000593519.1\|2094488_2094863_-	cholera enterotoxin binding subunit CtxB	A0A142I6Y1	Vibrio_phage	5.9e-65	100.0
WP_001888508.1\|2094859_2095636_-	cholera enterotoxin catalytic subunit CtxA	A0A023W6A1	Vibrio_virus	7.8e-152	100.0
WP_050926705.1\|2095735_2096935_-	zonula occludens toxin ZOT	A0A023W604	Vibrio_virus	5.3e-240	100.0
WP_000979342.1\|2096931_2097225_-	accessory cholera enterotoxin	A0A0F6YNQ7	Vibrio_phage	3.7e-46	100.0
WP_080482798.1\|2097221_2098502_-	hypothetical protein	A0A142I701	Vibrio_phage	3.3e-216	93.4
WP_000493022.1\|2098512_2098761_-	colonization factor	A0A0N7F140	Vibrio_phage	8.8e-33	100.0
WP_001888513.1\|2098896_2099277_-	hypothetical protein	A0A0N9HJ67	Vibrio_phage	4.0e-69	100.0
WP_019830347.1\|2099254_2100334_-	hypothetical protein	A0A023W5Z8	Vibrio_virus	2.2e-213	100.0
WP_000492997.1\|2100465_2100801_+	helix-turn-helix transcriptional regulator	A0A0N9HJE7	Vibrio_phage	4.7e-53	100.0
WP_158106483.1\|2101314_2102514_-	zonula occludens toxin ZOT	A0A0N9HE81	Vibrio_phage	1.2e-236	99.2
WP_000979342.1\|2102510_2102804_-	accessory cholera enterotoxin	A0A0F6YNQ7	Vibrio_phage	3.7e-46	100.0
WP_158106484.1\|2102800_2104081_-	hypothetical protein	A0A142I701	Vibrio_phage	1.8e-217	93.9
WP_000493022.1\|2104091_2104340_-	colonization factor	A0A0N7F140	Vibrio_phage	8.8e-33	100.0
WP_032473196.1\|2104475_2104796_-	hypothetical protein	Q2PZB5	Vibrio_virus	1.0e-49	92.5
WP_005521561.1\|2104845_2106591_-	replication endonuclease	A0A0F6YPA7	Vibrio_phage	0.0e+00	98.1
WP_032469109.1\|2107102_2107444_+	helix-turn-helix transcriptional regulator	A0A0F6WBJ9	Vibrio_phage	9.6e-54	100.0
WP_158106485.1\|2107879_2109079_-	zonula occludens toxin ZOT	A0A0N9HE81	Vibrio_phage	2.5e-237	99.5
WP_000979342.1\|2109075_2109369_-	accessory cholera enterotoxin	A0A0F6YNQ7	Vibrio_phage	3.7e-46	100.0
WP_158106486.1\|2109365_2110649_-	hypothetical protein	A0A142I701	Vibrio_phage	2.3e-217	98.4
WP_000493022.1\|2110659_2110908_-	colonization factor	A0A0N7F140	Vibrio_phage	8.8e-33	100.0
WP_032473196.1\|2111043_2111364_-	hypothetical protein	Q2PZB5	Vibrio_virus	1.0e-49	92.5
WP_005521561.1\|2111413_2113159_-	replication endonuclease	A0A0F6YPA7	Vibrio_phage	0.0e+00	98.1
WP_032469109.1\|2113670_2114012_+	helix-turn-helix transcriptional regulator	A0A0F6WBJ9	Vibrio_phage	9.6e-54	100.0
WP_158106485.1\|2114447_2115647_-	zonula occludens toxin ZOT	A0A0N9HE81	Vibrio_phage	2.5e-237	99.5
WP_000979342.1\|2115643_2115937_-	accessory cholera enterotoxin	A0A0F6YNQ7	Vibrio_phage	3.7e-46	100.0
WP_158106486.1\|2115933_2117217_-	hypothetical protein	A0A142I701	Vibrio_phage	2.3e-217	98.4
WP_000493022.1\|2117227_2117476_-	colonization factor	A0A0N7F140	Vibrio_phage	8.8e-33	100.0
WP_005521561.1\|2117982_2119728_-	replication endonuclease	A0A0F6YPA7	Vibrio_phage	0.0e+00	98.1
WP_032469109.1\|2120239_2120581_+	helix-turn-helix transcriptional regulator	A0A0F6WBJ9	Vibrio_phage	9.6e-54	100.0
WP_000288488.1\|2120968_2121331_-	hypothetical protein	Q9MCC3	Vibrio_phage	3.8e-32	58.5
WP_001018569.1\|2121509_2121758_+	hypothetical protein	O56843	Vibrio_virus	1.8e-22	65.9
WP_069648025.1\|2121768_2121972_+	hypothetical protein	E1CJD3	Vibrio_phage	3.5e-35	98.5
WP_001112192.1\|2121961_2123065_+	hypothetical protein	Q64EV8	Vibrio_phage	1.5e-220	100.0
WP_001284206.1\|2123069_2123408_+	DUF1293 domain-containing protein	Q858R2	Vibrio_phage	1.3e-55	96.4
WP_001900021.1\|2123417_2123648_+	hypothetical protein	G8IRU7	Vibrio_phage	3.9e-35	94.7
WP_000672170.1\|2123681_2123891_+	hypothetical protein	G8IRU8	Vibrio_phage	1.1e-20	81.2
WP_158106487.1\|2124023_2125418_+	hypothetical protein	Q64EV4	Vibrio_phage	5.2e-162	94.6
WP_001160095.1\|2125417_2125762_+	DUF2523 domain-containing protein	Q64EV1	Vibrio_phage	4.8e-61	100.0
WP_158106488.1\|2125764_2127132_+	toxin	Q64EV0	Vibrio_phage	7.8e-256	95.6
WP_032474068.1\|2127354_2127546_+	hypothetical protein	NA	NA	NA	NA
WP_148526835.1\|2128468_2130607_+	MarR family transcriptional regulator	Q64EU7	Vibrio_phage	2.8e-34	92.5

DBSCAN-SWA_7

2979471 : 2986664

Anguillid_herpesvirus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_001162850.1\|2979471_2979900_-	nucleoside-diphosphate kinase	D2E8E1	Anguillid_herpesvirus	1.0e-20	38.4
WP_001959105.1\|2980103_2981393_-	aminopeptidase PepB	Q6GYZ8	Mycoplasma_phage	6.5e-34	34.0
WP_000872174.1\|2981612_2981807_-	Fe-S cluster assembly protein IscX	NA	NA	NA	NA
WP_001124187.1\|2981855_2982194_-	ISC system 2Fe-2S type ferredoxin	NA	NA	NA	NA
WP_019830072.1\|2982207_2984058_-	Fe-S protein assembly chaperone HscA	A0A0N9QXN8	Chrysochromulina_ericina_virus	1.5e-105	41.1
WP_001105747.1\|2984081_2984597_-	co-chaperone HscB	NA	NA	NA	NA
WP_000301571.1\|2984645_2984969_-	iron-sulfur cluster assembly protein IscA	A0A2H4N7N5	Lake_Baikal_phage	3.0e-25	50.5
WP_000331703.1\|2985029_2985413_-	Fe-S cluster assembly scaffold IscU	A0A218MKD1	uncultured_virus	5.4e-53	78.2
WP_001886931.1\|2985449_2986664_-	IscS subfamily cysteine desulfurase	A0A1X7C038	Faustovirus	5.0e-28	41.8

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP046735

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NZ_CP046736

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection