CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_AP022345	Rhodocyclaceae bacterium ICHIAU1 chromosome, complete genome	0 crisprs	cas3,WYL,DEDDh,RT,DinG	0	0	6	0
NZ_AP022346	Rhodocyclaceae bacterium ICHIAU1 plasmid pICHIAU1, complete sequence	0 crisprs	NA	0	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

857069 : 864335

Bacillus_phage(33.33%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_162050645.1\|857069_858116_+	phosphate ABC transporter substrate-binding protein PstS	A0A1D7SRJ6	Cyanophage	1.3e-48	40.1
WP_162050999.1\|858239_859178_+	phosphate ABC transporter permease subunit PstC	NA	NA	NA	NA
WP_162050644.1\|859179_860025_+	phosphate ABC transporter permease PstA	NA	NA	NA	NA
WP_162050643.1\|860034_860817_+	phosphate ABC transporter ATP-binding protein PstB	G9BWD6	Planktothrix_phage	3.7e-16	30.4
WP_162050642.1\|860850_862080_-	phosphate regulon sensor histidine kinase PhoR	W8CYF6	Bacillus_phage	1.1e-30	33.3
WP_162050641.1\|862127_862820_-	phosphate regulon transcriptional regulatory protein PhoB	W8CYM9	Bacillus_phage	4.5e-34	35.7
WP_162050640.1\|862834_863548_-	phosphate signaling complex protein PhoU	NA	NA	NA	NA
WP_162050639.1\|863627_863942_-	DUF190 domain-containing protein	A0A2H4J2R0	uncultured_Caudovirales_phage	4.9e-20	42.3
WP_162050638.1\|863951_864335_-	fluoride efflux transporter CrcB	A0A2H4J148	uncultured_Caudovirales_phage	2.5e-34	64.2

DBSCAN-SWA_2

873552 : 883971

Vibrio_phage(16.67%)

tRNA

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_162050627.1\|873552_876099_-	RNA polymerase sigma factor RpoD	A0A2I7SAT0	Vibrio_phage	6.8e-35	32.3
WP_162050626.1\|876261_878037_-	DNA primase	A0A0K1LMQ9	Caulobacter_phage	3.2e-68	40.0
WP_162050625.1\|878058_878514_-	GatB/YqeY domain-containing protein	A0A292GL36	Xanthomonas_phage	3.4e-22	46.6
WP_162050624.1\|878602_878815_-	30S ribosomal protein S21	NA	NA	NA	NA
WP_162050623.1\|878965_880045_+\|tRNA	tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD	A0A0R6PI74	Moraxella_phage	7.0e-82	49.9
WP_162050998.1\|880050_880698_-	glycerol-3-phosphate 1-O-acyltransferase PlsY	NA	NA	NA	NA
WP_162050622.1\|880795_881146_+	dihydroneopterin aldolase	NA	NA	NA	NA
WP_162050621.1\|881233_882157_-	site-specific tyrosine recombinase XerD	A0A0K2CP59	Brevibacillus_phage	5.1e-33	30.4
WP_162050620.1\|882149_882713_-	methylated-DNA--[protein]-cysteine S-methyltransferase	NA	NA	NA	NA
WP_162050619.1\|882696_883971_-	glutamate-5-semialdehyde dehydrogenase	A0A1X9I5D4	Streptococcus_phage	3.8e-95	48.2

DBSCAN-SWA_3

1398649 : 1451282

Macacine_betaherpesvirus(25.0%)

transposase,integrase

attL 1409231:1409246|attR 1451956:1451971

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_162050170.1\|1398649_1399879_-\|transposase	IS256 family transposase	A0A218MNI5	uncultured_virus	4.2e-51	49.7
WP_162050169.1\|1400232_1400538_+	type II toxin-antitoxin system RelE/ParE family toxin	A0A141GEX6	Brucella_phage	3.4e-18	48.4
WP_162050168.1\|1400530_1400848_+	putative addiction module antidote protein	A4JWV1	Burkholderia_virus	4.5e-13	39.8
WP_162050167.1\|1400913_1401753_-	nucleotidyl transferase AbiEii/AbiGii toxin family protein	NA	NA	NA	NA
WP_162050166.1\|1401749_1402352_-	transcriptional regulator	NA	NA	NA	NA
WP_162050165.1\|1402441_1403464_-	hypothetical protein	NA	NA	NA	NA
WP_162071257.1\|1403917_1404061_+	hypothetical protein	NA	NA	NA	NA
WP_162071258.1\|1404157_1405372_+	hypothetical protein	NA	NA	NA	NA
WP_162071259.1\|1405477_1406077_-	class I SAM-dependent methyltransferase	NA	NA	NA	NA
WP_162071260.1\|1406089_1406476_-	rhodanese-like domain-containing protein	NA	NA	NA	NA
WP_162071261.1\|1406479_1407010_-	YeeE/YedE family protein	NA	NA	NA	NA
WP_162071262.1\|1407012_1407546_-	YeeE/YedE family protein	NA	NA	NA	NA
WP_162071263.1\|1407872_1409906_+	c-type cytochrome	NA	NA	NA	NA
1409231:1409246	attL	NA	NA	NA	NA
WP_162071264.1\|1409940_1410249_+	twin-arginine translocation signal domain-containing protein	NA	NA	NA	NA
WP_162071265.1\|1410490_1411501_-	hypothetical protein	NA	NA	NA	NA
WP_162050955.1\|1411747_1412404_-\|integrase	tyrosine-type recombinase/integrase	A0A1J0GUW7	Halomonas_phage	4.3e-26	31.6
WP_162050164.1\|1413661_1413943_+	hypothetical protein	NA	NA	NA	NA
WP_162050163.1\|1413946_1415047_+	P-type DNA transfer ATPase VirB11	NA	NA	NA	NA
WP_162050954.1\|1415162_1415396_+	hypothetical protein	NA	NA	NA	NA
WP_162050162.1\|1415392_1415767_+	MarR family transcriptional regulator	NA	NA	NA	NA
WP_162050161.1\|1415987_1416173_+	hypothetical protein	NA	NA	NA	NA
WP_162050160.1\|1416219_1416540_+	XRE family transcriptional regulator	NA	NA	NA	NA
WP_162050159.1\|1416660_1417188_-	transglycosylase SLT domain-containing protein	A0A0H3V0Q1	Geobacillus_virus	5.3e-19	45.9
WP_162050158.1\|1417194_1417335_-	hypothetical protein	NA	NA	NA	NA
WP_162050157.1\|1417407_1418247_-	alpha/beta fold hydrolase	NA	NA	NA	NA
WP_162050156.1\|1418584_1418788_-	hypothetical protein	NA	NA	NA	NA
WP_162050155.1\|1418993_1420880_-	CBS domain-containing protein	NA	NA	NA	NA
WP_162071266.1\|1421374_1421629_-	hypothetical protein	NA	NA	NA	NA
WP_162050153.1\|1423086_1423245_-	DUF2789 family protein	NA	NA	NA	NA
WP_162050152.1\|1423317_1424289_+	alpha/beta fold hydrolase	NA	NA	NA	NA
WP_162050151.1\|1424808_1425111_-	monooxygenase	NA	NA	NA	NA
WP_162050150.1\|1425114_1426188_-	NAD(P)-dependent alcohol dehydrogenase	M1PHA2	Moumouvirus	4.0e-05	23.3
WP_162050149.1\|1426422_1430019_+	S8 family serine peptidase	NA	NA	NA	NA
WP_162050148.1\|1430048_1430681_+	carbonic anhydrase	NA	NA	NA	NA
WP_162050138.1\|1430717_1431849_+\|transposase	IS3 family transposase	A0A2I6AZV9	Macacine_betaherpesvirus	1.6e-12	28.4
WP_162050147.1\|1432003_1432396_+	hypothetical protein	NA	NA	NA	NA
WP_162050953.1\|1432579_1432897_+	HNH endonuclease	NA	NA	NA	NA
WP_162050146.1\|1433168_1433450_-	hypothetical protein	NA	NA	NA	NA
WP_162050145.1\|1433527_1433758_-	DUF2789 domain-containing protein	NA	NA	NA	NA
WP_162050144.1\|1434286_1435480_-	MFS transporter	NA	NA	NA	NA
WP_162050952.1\|1435619_1436606_-	ZIP family metal transporter	NA	NA	NA	NA
WP_162050143.1\|1436740_1437499_+	ferredoxin--NADP reductase	NA	NA	NA	NA
WP_162050142.1\|1438264_1439608_-	NADP-specific glutamate dehydrogenase	NA	NA	NA	NA
WP_162050141.1\|1439771_1440713_+	sodium-dependent bicarbonate transport family permease	NA	NA	NA	NA
WP_162049263.1\|1441143_1441821_+\|transposase	IS1595 family transposase	NA	NA	NA	NA
WP_162050140.1\|1441885_1443589_-	sodium:proton antiporter	NA	NA	NA	NA
WP_162050139.1\|1443970_1444897_-	universal stress protein	NA	NA	NA	NA
WP_162050138.1\|1444994_1446127_-\|transposase	IS3 family transposase	A0A2I6AZV9	Macacine_betaherpesvirus	1.6e-12	28.4
WP_162050137.1\|1447638_1447803_+	hypothetical protein	NA	NA	NA	NA
WP_162050136.1\|1448035_1449079_-	hypothetical protein	NA	NA	NA	NA
WP_162071267.1\|1449128_1449443_-	DUF59 domain-containing protein	NA	NA	NA	NA
WP_162050134.1\|1449604_1450069_+	hypothetical protein	NA	NA	NA	NA
WP_162050133.1\|1450118_1451282_-\|transposase	IS4 family transposase	NA	NA	NA	NA
1451956:1451971	attR	NA	NA	NA	NA

DBSCAN-SWA_4

1616379 : 1674233

Acidithiobacillus_phage(27.27%)

tRNA,transposase,integrase

attL 1662915:1662931|attR 1674261:1674277

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_162071291.1\|1616379_1617225_+\|tRNA	tRNA pseudouridine(38-40) synthase TruA	NA	NA	NA	NA
WP_162049962.1\|1617235_1617862_+	phosphoribosylanthranilate isomerase	NA	NA	NA	NA
WP_162071292.1\|1617918_1619193_+	tryptophan synthase subunit beta	NA	NA	NA	NA
WP_162071293.1\|1619192_1620005_+	tryptophan synthase subunit alpha	NA	NA	NA	NA
WP_162071294.1\|1620028_1620895_+	acetyl-CoA carboxylase carboxyltransferase subunit beta	NA	NA	NA	NA
WP_162071295.1\|1620914_1622255_+	bifunctional folylpolyglutamate synthase/dihydrofolate synthase	NA	NA	NA	NA
WP_162071296.1\|1622251_1623025_+	hypothetical protein	NA	NA	NA	NA
WP_162071297.1\|1623046_1623604_+	CvpA family protein	NA	NA	NA	NA
WP_162071298.1\|1623616_1625170_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	1.9e-93	43.2
WP_162071299.1\|1625181_1626375_+	O-succinylhomoserine sulfhydrylase	NA	NA	NA	NA
WP_162071300.1\|1626443_1628405_+	DEAD/DEAH box helicase	Q5GF26	Diachasmimorpha_longicaudata_entomopoxvirus	1.1e-32	26.3
WP_162071301.1\|1628783_1629050_+	AlpA family phage regulatory protein	NA	NA	NA	NA
WP_162071302.1\|1629138_1630032_+	hypothetical protein	NA	NA	NA	NA
WP_162071303.1\|1630128_1631040_+	hypothetical protein	NA	NA	NA	NA
WP_162071304.1\|1631165_1631939_+	FRG domain-containing protein	NA	NA	NA	NA
WP_162071305.1\|1632080_1633265_+	hypothetical protein	NA	NA	NA	NA
WP_162050099.1\|1633377_1634712_+\|transposase	IS5 family transposase	K4ICS3	Acidithiobacillus_phage	3.3e-57	49.2
WP_162071306.1\|1634932_1635550_+	hypothetical protein	NA	NA	NA	NA
WP_162071307.1\|1635566_1637435_+	DUF4209 domain-containing protein	NA	NA	NA	NA
WP_162071308.1\|1638102_1638636_+	hypothetical protein	NA	NA	NA	NA
WP_162071309.1\|1638686_1639007_+	hypothetical protein	NA	NA	NA	NA
WP_162071363.1\|1639212_1640157_+	ATP-binding protein	NA	NA	NA	NA
WP_162071310.1\|1640169_1641406_+\|transposase	IS3 family transposase	Q8W6R2	Burkholderia_virus	2.6e-125	76.0
WP_162071311.1\|1641706_1642051_+	type II toxin-antitoxin system RelE/ParE family toxin	NA	NA	NA	NA
WP_162049948.1\|1642047_1642338_+	XRE family transcriptional regulator	NA	NA	NA	NA
WP_162071312.1\|1642673_1643348_+	HNH endonuclease	NA	NA	NA	NA
WP_162071313.1\|1643422_1644391_+	hypothetical protein	NA	NA	NA	NA
WP_162071314.1\|1644564_1645533_-	nucleotidyl transferase AbiEii/AbiGii toxin family protein	NA	NA	NA	NA
WP_162071364.1\|1645516_1645921_-	S-adenosylhomocysteine hydrolase	NA	NA	NA	NA
WP_162071315.1\|1646331_1648428_-	hypothetical protein	NA	NA	NA	NA
WP_162071316.1\|1648704_1649280_-	hypothetical protein	NA	NA	NA	NA
WP_162049946.1\|1649445_1650198_-	ATP-binding protein	K4HZD4	Acidithiobacillus_phage	4.4e-83	61.2
WP_162050093.1\|1650211_1651750_-\|transposase	IS21 family transposase	K4I413	Acidithiobacillus_phage	8.5e-166	58.6
WP_162071317.1\|1651857_1652235_-	hypothetical protein	NA	NA	NA	NA
WP_162071318.1\|1652231_1653299_-	DUF262 domain-containing protein	A0A2P0VN93	Tetraselmis_virus	3.5e-09	34.5
WP_162071319.1\|1653610_1654918_-\|integrase	tyrosine-type recombinase/integrase	B7SYF8	Stenotrophomonas_phage	2.7e-19	26.0
WP_162071320.1\|1655207_1656191_+	oxidoreductase	NA	NA	NA	NA
WP_162071321.1\|1656219_1657179_-	hypothetical protein	NA	NA	NA	NA
WP_162049931.1\|1657253_1657685_+	hypothetical protein	NA	NA	NA	NA
WP_162049930.1\|1657700_1658168_-	6-carboxytetrahydropterin synthase QueD	NA	NA	NA	NA
WP_162049929.1\|1658218_1659097_+	radical SAM protein	NA	NA	NA	NA
WP_162050936.1\|1659097_1659376_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_162049928.1\|1659637_1660873_+	aspartate kinase	NA	NA	NA	NA
WP_162049927.1\|1661142_1661661_-	DUF1643 domain-containing protein	NA	NA	NA	NA
WP_162071322.1\|1661849_1662758_-	5'-nucleotidase	NA	NA	NA	NA
1662915:1662931	attL	NA	NA	NA	NA
WP_162049925.1\|1663201_1663936_+	hypothetical protein	NA	NA	NA	NA
WP_162049924.1\|1663932_1664691_+	hypothetical protein	NA	NA	NA	NA
WP_162049923.1\|1664821_1665217_+	hypothetical protein	NA	NA	NA	NA
WP_162049922.1\|1665206_1665800_+	hypothetical protein	NA	NA	NA	NA
WP_162049921.1\|1665839_1667174_+	ATP-binding protein	NA	NA	NA	NA
WP_162049920.1\|1667931_1668189_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_162049919.1\|1668273_1668954_+	hypothetical protein	NA	NA	NA	NA
WP_162071226.1\|1669064_1670108_-\|transposase	IS481 family transposase	A0A0M3LS26	Mannheimia_phage	2.4e-148	74.0
WP_162049918.1\|1670273_1670639_-	hypothetical protein	NA	NA	NA	NA
WP_162049917.1\|1670656_1671733_-	AAA family ATPase	A0A0N7CDQ9	Skermania_phage	2.5e-23	36.6
WP_162050935.1\|1671732_1671897_-	AlpA family phage regulatory protein	NA	NA	NA	NA
WP_162049916.1\|1672096_1672789_-	hypothetical protein	NA	NA	NA	NA
WP_162049915.1\|1672991_1674233_-\|integrase	site-specific integrase	A0A077KET4	Ralstonia_phage	1.4e-97	48.4
1674261:1674277	attR	NA	NA	NA	NA

DBSCAN-SWA_5

1824420 : 1832889

Pandoravirus(16.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_162049801.1\|1824420_1825503_+	3-deoxy-7-phosphoheptulonate synthase	S4W5F1	Pandoravirus	3.1e-82	45.6
WP_162049799.1\|1825508_1826354_+	phosphoserine phosphatase SerB	NA	NA	NA	NA
WP_162049797.1\|1826372_1826612_-	DUF2789 domain-containing protein	NA	NA	NA	NA
WP_162050925.1\|1826663_1826855_-	Fe-S cluster assembly protein IscX	NA	NA	NA	NA
WP_162049795.1\|1826864_1827206_-	ISC system 2Fe-2S type ferredoxin	NA	NA	NA	NA
WP_162049792.1\|1827202_1829125_-	Fe-S protein assembly chaperone HscA	F2Y2E1	Organic_Lake_phycodnavirus	2.3e-104	39.3
WP_162049790.1\|1829155_1829728_-	Fe-S protein assembly co-chaperone HscB	NA	NA	NA	NA
WP_162049788.1\|1829720_1830044_-	iron-sulfur cluster assembly protein IscA	A0A2H4N7N5	Lake_Baikal_phage	1.4e-22	48.6
WP_162049787.1\|1830057_1830441_-	Fe-S cluster assembly scaffold IscU	A0A218MKD1	uncultured_virus	5.4e-53	81.5
WP_162049786.1\|1830471_1831683_-	IscS subfamily cysteine desulfurase	A0A1X7C038	Faustovirus	5.9e-29	29.4
WP_162050924.1\|1831701_1832889_-	aminotransferase class V-fold PLP-dependent enzyme	H7BUW1	unidentified_phage	1.2e-29	40.4

DBSCAN-SWA_6

2291708 : 2302116

Ralstonia_phage(12.5%)

integrase

attL 2288109:2288123|attR 2308185:2308199

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
2288109:2288123	attL	NA	NA	NA	NA
WP_162049384.1\|2291708_2292947_-\|integrase	tyrosine-type recombinase/integrase	A0A077KET4	Ralstonia_phage	4.2e-91	47.2
WP_162049383.1\|2293253_2294051_-	3'-5' exonuclease	NA	NA	NA	NA
WP_162049382.1\|2294074_2294989_-	cysteine synthase CysM	A0A1X9I5K7	Streptococcus_phage	2.1e-55	41.8
WP_162049381.1\|2294966_2295971_-	ADP-glyceromanno-heptose 6-epimerase	E3SL51	Synechococcus_phage	1.5e-30	34.4
WP_162049380.1\|2296010_2296940_-	D-glycero-beta-D-manno-heptose-7-phosphate kinase	R9S8D5	Prochlorococcus_phage	2.8e-15	26.5
WP_162049379.1\|2296967_2297864_-	dTDP-4-dehydrorhamnose reductase	A0A291LA50	Escherichia_phage	5.7e-29	34.2
WP_162049378.1\|2297866_2298937_-	dTDP-glucose 4,6-dehydratase	I7HTA3	Enterobacteria_phage	1.3e-91	49.9
WP_162049377.1\|2298936_2300106_-	nucleotide sugar dehydrogenase	O41091	Paramecium_bursaria_Chlorella_virus	2.8e-113	56.6
WP_162050882.1\|2300187_2301354_-	lipopolysaccharide assembly protein LapB	NA	NA	NA	NA
WP_162049376.1\|2301367_2301667_-	DUF1049 domain-containing protein	NA	NA	NA	NA
WP_162049375.1\|2301747_2302116_-	integration host factor subunit beta	A7KV42	Bacillus_phage	4.4e-12	44.4
2308185:2308199	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage