CRISPRimmunity

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Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Self targeting spacer number	Target MGE spacer number	Prophage number	Anti-CRISPR protein number
NZ_CP051011	Bacillus velezensis strain BY6 chromosome, complete genome	0 crisprs	csa3,DinG,cas3,DEDDh,WYL	0	0	6	0
NZ_CP051012	Bacillus velezensis strain BY6 plasmid unnamed1, complete sequence	0 crisprs	NA	0	0	0	0

Results visualization

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CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

647624 : 657515

Synechococcus_phage(50.0%)

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_003155764.1\|647624_648917_+	adenylosuccinate lyase	A0A2H4UUU6	Bodo_saltans_virus	4.5e-19	27.3
WP_003155762.1\|648992_649712_+	phosphoribosylaminoimidazolesuccinocarboxamide synthase	A0A0E3G7P8	Synechococcus_phage	5.2e-49	45.2
WP_003155758.1\|649711_649966_+	phosphoribosylformylglycinamidine synthase subunit PurS	M4QPE7	Synechococcus_phage	4.7e-05	33.3
WP_014304545.1\|649962_650646_+	phosphoribosylformylglycinamidine synthase subunit PurQ	NA	NA	NA	NA
WP_069473179.1\|650629_652858_+	phosphoribosylformylglycinamidine synthase subunit PurL	A6N228	Microbacterium_phage	1.5e-158	40.5
WP_015388589.1\|652833_654264_+	amidophosphoribosyltransferase	A0A0M3SGR2	Mollivirus	1.6e-54	32.9
WP_069473180.1\|654355_655396_+	phosphoribosylformylglycinamidine cyclo-ligase	A0A0E3F760	Synechococcus_phage	5.3e-63	41.1
WP_003155752.1\|655392_655980_+	phosphoribosylglycinamide formyltransferase	M4QRX9	Synechococcus_phage	1.0e-26	39.3
WP_043866892.1\|655976_657515_+	bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase	Q58MG4	Prochlorococcus_phage	8.7e-78	50.8

DBSCAN-SWA_2

1204518 : 1236324

Bacillus_phage(33.33%)

portal,plate,holin,terminase,tail

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_087920760.1\|1204518_1205655_+	tetratricopeptide repeat protein	A0A1P8CWN8	Bacillus_phage	1.3e-94	47.9
WP_003154881.1\|1205644_1205779_+	PhrA family phosphatase inhibitor	NA	NA	NA	NA
WP_003154880.1\|1205921_1206875_+	LysM peptidoglycan-binding domain-containing protein	A0A218KC88	Bacillus_phage	1.1e-62	69.3
WP_003154878.1\|1206912_1207290_-	PH domain-containing protein	A5GYQ0	Lactococcus_phage	1.1e-15	40.6
WP_003154876.1\|1207389_1207995_+	poly-gamma-glutamate hydrolase family protein	A0A0Y0AJU6	Bacillus_phage	7.4e-41	46.8
WP_003154875.1\|1207984_1208134_+	hypothetical protein	NA	NA	NA	NA
WP_003154873.1\|1208149_1208740_-	ImmA/IrrE family metallo-endopeptidase	A0A2H4JA43	uncultured_Caudovirales_phage	3.0e-39	51.7
WP_003154871.1\|1208888_1209227_-	helix-turn-helix transcriptional regulator	A0A2H4J6F9	uncultured_Caudovirales_phage	8.4e-18	45.8
WP_003154869.1\|1209418_1209598_+	hypothetical protein	NA	NA	NA	NA
WP_003154867.1\|1209587_1210415_+\|portal	phage portal protein	S6BFM4	Thermus_phage	6.4e-19	27.9
WP_003154865.1\|1210314_1211115_+	ATP-binding protein	A6XMI1	Bacillus_virus	3.6e-59	45.1
WP_003154863.1\|1211114_1211282_+	hypothetical protein	NA	NA	NA	NA
WP_003154861.1\|1211379_1211721_+	hypothetical protein	NA	NA	NA	NA
WP_003154859.1\|1211710_1211914_+	hypothetical protein	A0A2H4J4M6	uncultured_Caudovirales_phage	5.4e-12	48.5
WP_069473351.1\|1212027_1212540_+	sigma-70 family RNA polymerase sigma factor	A0A0K2CNQ1	Brevibacillus_phage	2.9e-22	43.9
WP_165408939.1\|1212652_1213450_+\|terminase	terminase	A0A0S2MVB6	Bacillus_phage	7.2e-60	49.8
WP_039251791.1\|1213446_1214745_+\|terminase	PBSX family phage terminase large subunit	M4ZRM5	Bacillus_phage	1.2e-149	58.9
WP_154021550.1\|1214793_1216185_+\|portal	phage portal protein	A0A1B1P7C8	Bacillus_phage	5.2e-138	55.4
WP_015417286.1\|1216204_1217050_+	hypothetical protein	A0A1B1P7E4	Bacillus_phage	4.3e-55	57.6
WP_007407274.1\|1217076_1218012_+\|portal	phage portal protein	A0A1B1P7E3	Bacillus_phage	3.3e-104	62.8
WP_069473355.1\|1218028_1218412_+	DUF3199 family protein	NA	NA	NA	NA
WP_007610806.1\|1218408_1218765_+	DUF3599 family protein	NA	NA	NA	NA
WP_069473356.1\|1218761_1219265_+	HK97 gp10 family phage protein	A0A249XXA4	Clostridium_phage	3.2e-37	41.0
WP_069473357.1\|1219261_1219708_+\|portal	phage portal protein	NA	NA	NA	NA
WP_063095512.1\|1219704_1219914_+	hypothetical protein	NA	NA	NA	NA
WP_069473358.1\|1219913_1221311_+\|tail	phage tail sheath protein	A0A0A7RTT5	Clostridium_phage	2.0e-81	40.2
WP_003154837.1\|1221312_1221756_+\|tail	phage tail tube protein	A0A0K2CNG3	Brevibacillus_phage	8.4e-26	45.2
WP_032874591.1\|1221831_1222278_+\|portal	phage portal protein	A0A0A7RTY8	Clostridium_phage	1.0e-10	35.8
WP_015239684.1\|1222319_1222472_+	hypothetical protein	NA	NA	NA	NA
WP_069473359.1\|1222459_1227502_+	transglycosylase SLT domain-containing protein	A0A1L2JY60	Aeribacillus_phage	1.9e-41	46.6
WP_024085190.1\|1227494_1228154_+	LysM peptidoglycan-binding domain-containing protein	G3MBQ1	Bacillus_virus	1.8e-08	45.3
WP_058905959.1\|1228167_1229145_+	hypothetical protein	A0A1L6BY20	Clostridium_phage	1.9e-33	30.6
WP_003154827.1\|1229144_1229411_+	DUF2577 family protein	S6C459	Thermus_phage	3.4e-06	37.5
WP_003154825.1\|1229514_1229940_+	DUF2634 domain-containing protein	A0A2H4J6K5	uncultured_Caudovirales_phage	1.5e-11	35.9
WP_003154824.1\|1229932_1230979_+\|plate	baseplate J/gp47 family protein	S6AVU3	Thermus_phage	7.7e-70	44.1
WP_007407262.1\|1230962_1231541_+	YmfQ family protein	A0A2H4J717	uncultured_Caudovirales_phage	3.1e-12	31.0
WP_069473360.1\|1231537_1231810_+	hypothetical protein	NA	NA	NA	NA
WP_069473361.1\|1231812_1233435_+	hypothetical protein	A0A1P8CWR7	Bacillus_phage	4.0e-41	39.3
WP_168237132.1\|1233447_1233819_+	hypothetical protein	NA	NA	NA	NA
WP_168237133.1\|1233823_1234021_+	XkdX family protein	NA	NA	NA	NA
WP_003154815.1\|1234891_1235155_+	protein xhlA	A0A2H4JD40	uncultured_Caudovirales_phage	8.8e-23	63.1
WP_003154813.1\|1235168_1235432_+\|holin	phage holin	A0A2H4J6M0	uncultured_Caudovirales_phage	2.3e-23	65.5
WP_168237134.1\|1235445_1236324_+	LysM peptidoglycan-binding domain-containing protein	Q9ZXD7	Bacillus_phage	2.8e-81	60.5

DBSCAN-SWA_3

1790854 : 1797068

Bacillus_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_014305044.1\|1790854_1791247_+	class Ib ribonucleoside-diphosphate reductase assembly flavoprotein NrdI	G3MBF1	Bacillus_virus	6.5e-30	59.1
WP_003154060.1\|1791206_1793309_+	class 1b ribonucleoside-diphosphate reductase subunit alpha	U5PY35	Bacillus_phage	0.0e+00	86.0
WP_024085331.1\|1793326_1794316_+	class 1b ribonucleoside-diphosphate reductase subunit beta	F8WQ21	Bacillus_phage	2.5e-155	83.3
WP_046341259.1\|1794364_1794985_+	hypothetical protein	A0A127AYS1	Bacillus_phage	7.9e-46	46.9
WP_168237147.1\|1795034_1795793_-	N-acetylmuramoyl-L-alanine amidase	E5DV68	Deep-sea_thermophilic_phage	5.3e-52	50.4
WP_057080722.1\|1795826_1796051_-	hypothetical protein	NA	NA	NA	NA
WP_015417523.1\|1796099_1797068_+	stage V sporulation protein K	G3MAX6	Bacillus_virus	8.0e-53	40.9

DBSCAN-SWA_4

2088033 : 2101181

Bacillus_phage(90.91%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_024085504.1\|2088033_2088408_+	hypothetical protein	A0A1P8CWZ2	Bacillus_phage	2.8e-30	52.8
WP_025852486.1\|2088654_2089107_-	hypothetical protein	O64117	Bacillus_phage	3.7e-61	77.3
WP_043867220.1\|2089470_2090607_+	tetratricopeptide repeat protein	A0A1P8CWN8	Bacillus_phage	3.1e-165	76.4
WP_003153655.1\|2090596_2090779_+	hypothetical protein	NA	NA	NA	NA
WP_043867221.1\|2091104_2092124_+	hypothetical protein	NA	NA	NA	NA
WP_014305240.1\|2092184_2092544_-	hypothetical protein	O64028	Bacillus_phage	3.7e-32	60.5
WP_024085506.1\|2092549_2093017_-	DUF4879 domain-containing protein	O64027	Bacillus_phage	1.0e-42	57.1
WP_014305242.1\|2093575_2093914_-	hypothetical protein	A0A1P8CWM9	Bacillus_phage	1.1e-25	56.2
WP_014305245.1\|2094675_2095272_-	SMI1/KNR4 family protein	O64025	Bacillus_phage	9.4e-89	84.6
WP_014305246.1\|2095326_2095785_-	SMI1/KNR4 family protein	NA	NA	NA	NA
WP_043867222.1\|2095799_2097599_-	ribonuclease YeeF family protein	A0A1P8CWI7	Bacillus_phage	1.7e-170	64.8
WP_043867641.1\|2097697_2098255_-	SMI1/KNR4 family protein	O64022	Bacillus_phage	1.4e-89	82.7
WP_041482372.1\|2098534_2098843_-	hypothetical protein	NA	NA	NA	NA
WP_069473456.1\|2098900_2100568_+	recombinase family protein	O64015	Bacillus_phage	6.6e-273	89.2
WP_032857070.1\|2100590_2101181_+	polysaccharide biosynthesis protein	A0A2P1ELS8	Moumouvirus	2.9e-13	31.1

DBSCAN-SWA_5

2234956 : 2241209

Staphylococcus_phage(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_003153378.1\|2234956_2235550_-	SMC-Scp complex subunit ScpB	A0A1B1IVT7	uncultured_Mediterranean_phage	2.6e-14	36.6
WP_003153377.1\|2235539_2236295_-	segregation/condensation protein A	A0A1B1IVW1	uncultured_Mediterranean_phage	6.5e-10	25.3
WP_003153376.1\|2236502_2236592_+	YjcZ family sporulation protein	NA	NA	NA	NA
WP_012117889.1\|2236679_2237201_+	DUF309 domain-containing protein	NA	NA	NA	NA
WP_003153373.1\|2237266_2237641_-	GNAT family N-acetyltransferase RibT	NA	NA	NA	NA
WP_003153372.1\|2237757_2238222_-	6,7-dimethyl-8-ribityllumazine synthase	A0A2H4PQS3	Staphylococcus_phage	1.2e-43	59.3
WP_032857112.1\|2238254_2239451_-	bifunctional 3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP cyclohydrolase II	A0A2H4PQS2	Staphylococcus_phage	8.2e-116	54.9
WP_032857114.1\|2239465_2240113_-	riboflavin synthase	A0A2H4PQS5	Staphylococcus_phage	3.3e-39	44.3
WP_024085544.1\|2240093_2241209_-	bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD	A0A2H4PQS8	Staphylococcus_phage	5.2e-56	36.9

DBSCAN-SWA_6

3566503 : 3611619

Cafeteria_roenbergensis_virus(11.11%)

protease,coat

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_014306025.1\|3566503_3567163_-\|protease	site-2 protease family protein	NA	NA	NA	NA
WP_003151042.1\|3567268_3567457_+	4-oxalocrotonate tautomerase	NA	NA	NA	NA
WP_003151040.1\|3567494_3567914_-	MarR family transcriptional regulator	NA	NA	NA	NA
WP_076982818.1\|3568289_3569684_+	amino acid permease	NA	NA	NA	NA
WP_007407653.1\|3569748_3570249_-	YwgA family protein	NA	NA	NA	NA
WP_025853614.1\|3570288_3571590_-	HD domain-containing protein	E3T4P8	Cafeteria_roenbergensis_virus	1.0e-23	29.5
WP_003151034.1\|3571750_3571975_-	DUF1450 domain-containing protein	NA	NA	NA	NA
WP_003151032.1\|3572179_3572953_+	RsfA family transcriptional regulator	NA	NA	NA	NA
WP_012118696.1\|3573214_3573529_+	PadR family transcriptional regulator	NA	NA	NA	NA
WP_012118697.1\|3573529_3574084_+	DUF1700 domain-containing protein	NA	NA	NA	NA
WP_031378569.1\|3574181_3575102_+	ATP-binding cassette domain-containing protein	A0A2H4PQG7	Staphylococcus_phage	3.8e-36	38.3
WP_069473628.1\|3575098_3576052_+	ABC transporter permease	NA	NA	NA	NA
WP_003151024.1\|3576041_3576878_-	hypothetical protein	NA	NA	NA	NA
WP_014306029.1\|3576868_3577666_-	hypothetical protein	NA	NA	NA	NA
WP_069473629.1\|3577634_3578558_-	hypothetical protein	NA	NA	NA	NA
WP_003151018.1\|3578606_3578786_-	hypothetical protein	NA	NA	NA	NA
WP_003151016.1\|3578937_3579801_-	lipoate--protein ligase family protein	NA	NA	NA	NA
WP_003151014.1\|3579847_3580747_-	LysR family transcriptional regulator	A0A2H4J8I9	uncultured_Caudovirales_phage	2.1e-07	40.2
WP_015387551.1\|3580862_3581840_+	putative sulfate exporter family transporter	NA	NA	NA	NA
WP_003151012.1\|3581876_3582848_-	phosphate acetyltransferase	NA	NA	NA	NA
WP_003151011.1\|3583110_3583875_+	heme-dependent peroxidase	NA	NA	NA	NA
WP_024085927.1\|3583994_3584774_+	SDR family oxidoreductase	NA	NA	NA	NA
WP_014306032.1\|3584790_3585990_-	pyridoxal phosphate-dependent aminotransferase	NA	NA	NA	NA
WP_003151007.1\|3586002_3587184_-	MFS transporter	NA	NA	NA	NA
WP_048367779.1\|3587180_3588599_-	ATP-grasp domain-containing protein	NA	NA	NA	NA
WP_003151003.1\|3588616_3589378_-	dihydroanticapsin 7-dehydrogenase	Q06VL0	Trichoplusia_ni_ascovirus	4.4e-22	30.5
WP_003151000.1\|3589374_3590085_-	cupin domain-containing protein	NA	NA	NA	NA
WP_003150997.1\|3590074_3590689_-	bacilysin biosynthesis protein BacA	NA	NA	NA	NA
WP_044052810.1\|3590850_3592089_-	MFS transporter	NA	NA	NA	NA
WP_069473630.1\|3592311_3593514_+	multidrug effflux MFS transporter	S4TR35	Salmonella_phage	1.1e-27	26.1
WP_014306038.1\|3593546_3594965_-	amino acid permease	NA	NA	NA	NA
WP_069473631.1\|3594984_3596667_-	M20/M25/M40 family metallo-hydrolase	NA	NA	NA	NA
WP_003150992.1\|3596738_3598286_-	L-glutamate gamma-semialdehyde dehydrogenase	NA	NA	NA	NA
WP_025853627.1\|3598493_3599780_-	Glu/Leu/Phe/Val dehydrogenase	NA	NA	NA	NA
WP_003150988.1\|3599965_3600427_-	hypothetical protein	NA	NA	NA	NA
WP_069473632.1\|3600642_3601098_-\|coat	spore coat protein	NA	NA	NA	NA
WP_069473633.1\|3601094_3601943_-	dTDP-4-dehydrorhamnose reductase	A0A291LA50	Escherichia_phage	4.1e-37	37.3
WP_014306044.1\|3601963_3602911_-	dTDP-glucose 4,6-dehydratase	I7HTA3	Enterobacteria_phage	7.7e-69	42.9
WP_014306045.1\|3602913_3603651_-	NTP transferase domain-containing protein	G3MA50	Bacillus_virus	6.5e-47	42.7
WP_069473634.1\|3603678_3604683_-\|coat	spore coat protein	NA	NA	NA	NA
WP_015387540.1\|3604684_3605425_-\|coat	spore coat protein	NA	NA	NA	NA
WP_025853630.1\|3605417_3606539_-\|coat	spore coat protein	NA	NA	NA	NA
WP_071392442.1\|3606538_3607402_-	GNAT family N-acetyltransferase	NA	NA	NA	NA
WP_071392443.1\|3607402_3608572_-	DegT/DnrJ/EryC1/StrS family aminotransferase	NA	NA	NA	NA
WP_069013129.1\|3608593_3610018_-	CDP-glycerol glycerophosphotransferase family protein	NA	NA	NA	NA
WP_024085938.1\|3610022_3610793_-	glycosyltransferase family 2 protein	A0A0F7L2F7	uncultured_marine_virus	4.4e-06	26.3
WP_003150971.1\|3611073_3611619_+\|coat	spore coat protein GerQ	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage