CRISPRimmunity

Please click to download your results

Overview of predicted results

Overview of the results

Contig_ID	Contig_def	CRISPR array number	Contig Signature genes	Target MGE spacer number	Prophage number
NZ_CP054603	Sulfitobacter pseudonitzschiae strain H46 plasmid unnamed4, complete sequence	0 crisprs	NA	0	0
NZ_CP054605	Sulfitobacter pseudonitzschiae strain H46 plasmid unnamed6, complete sequence	0 crisprs	RT	0	0
NZ_CP054606	Sulfitobacter pseudonitzschiae strain H46 plasmid unnamed7, complete sequence	0 crisprs	NA	0	0
NZ_CP054607	Sulfitobacter pseudonitzschiae strain H46 plasmid unnamed8, complete sequence	0 crisprs	NA	0	0
NZ_CP054601	Sulfitobacter pseudonitzschiae strain H46 plasmid unnamed2, complete sequence	0 crisprs	NA	0	0
NZ_CP054600	Sulfitobacter pseudonitzschiae strain H46 plasmid unnamed1, complete sequence	0 crisprs	NA	0	0
NZ_CP054599	Sulfitobacter pseudonitzschiae strain H46 chromosome, complete genome	2 crisprs	WYL,csa3,DEDDh,cas3,RT	1	2
NZ_CP054604	Sulfitobacter pseudonitzschiae strain H46 plasmid unnamed5, complete sequence	0 crisprs	csa3	0	20
NZ_CP054608	Sulfitobacter pseudonitzschiae strain H46 plasmid unnamed9, complete sequence	0 crisprs	csa3	0	0
NZ_CP054602	Sulfitobacter pseudonitzschiae strain H46 plasmid unnamed3, complete sequence	0 crisprs	NA	0	0

Results visualization

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Crispr_ID: NZ_CP054599_1

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP054599_1

165743-165835

Orphan

Consensus_repeat	Method
CCCATGCCCGAGCAGGTCGGACAGGTG	CRISPRCasFinder

1 spacers

The CRISPR arrays of NZ_CP054599_1

>merge|NZ_CP054599|1|165743-165835|CRISPRCasFinder
CCCATGCCCGAGCAGGTCGGACAGGTGCGTTCCACGGTGAAAAAGCCCTGTTGCGCGCGGACCTTGCCCATGCCCGAGCACGTCGGACAGGTG

>NZ_CP054599|1|1|165743-165835|CRISPRCasFinder
CCCATGCCCGAGCAGGTCGGACAGGTG	CGTTCCACGGTGAAAAAGCCCTGTTGCGCGCGGACCTTG
CCCATGCCCGAGCACGTCGGACAGGTG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP054599.1\|WP_037922476.1\|152565_155058_+\|translation-initiation-factor-IF-2	unknown	unknown	gnl\|CDD\|235401
NZ_CP054599.1\|WP_037922473.1\|155392_155791_-\|(deoxy)nucleoside-triphosphate-pyrophosphohydrolase	unknown	unknown	gnl\|CDD\|239517
NZ_CP054599.1\|WP_037922466.1\|158068_160777_+\|preprotein-translocase-subunit-SecA	unknown	unknown	gnl\|CDD\|237259
NZ_CP054599.1\|WP_037922448.1\|171458_172262_+\|3-deoxy-manno-octulosonate-cytidylyltransferase	unknown	unknown	gnl\|CDD\|235473
NZ_CP054599.1\|WP_037922450.1\|170661_171459_+\|3'(2'),5'-bisphosphate-nucleotidase-CysQ	unknown	unknown	gnl\|CDD\|238816
NZ_CP054599.1\|WP_037922442.1\|174554_175541_+\|UDP-glucose-4-epimerase-GalE	unknown	unknown	gnl\|CDD\|224012
NZ_CP054599.1\|WP_037922462.1\|161909_163469_+\|substrate-binding-domain-containing-protein	unknown	unknown	gnl\|CDD\|270371
NZ_CP054599.1\|WP_051693968.1\|169119_169722_-\|hypothetical-protein	unknown	unknown	gnl\|CDD\|377965
NZ_CP054599.1\|WP_037922456.1\|166421_168332_-\|molecular-chaperone-DnaK	unknown	unknown	gnl\|CDD\|234715
NZ_CP054599.1\|WP_037922477.1\|151933_152530_+\|RNA-binding-protein	unknown	unknown	gnl\|CDD\|236401
NZ_CP054599.1\|WP_081855332.1\|175620_176733_+\|glycosyltransferase	unknown	unknown	gnl\|CDD\|340838
NZ_CP054599.1\|WP_143186733.1\|172159_173491_+\|glycosyltransferase	unknown	unknown	gnl\|CDD\|133029
NZ_CP054599.1\|WP_037922452.1\|169718_170543_-\|ABC-transporter-permease	unknown	unknown	gnl\|CDD\|185098
NZ_CP054599.1\|WP_037922464.1\|160895_161888_+\|hypothetical-protein	unknown	unknown	unknown
NZ_CP054599.1\|WP_051693970.1\|164294_165104_-\|methyltransferase-domain-containing-protein	unknown	unknown	gnl\|CDD\|369777
NZ_CP054599.1\|WP_037922480.1\|150289_151912_+\|transcription-termination/antitermination-protein-NusA	unknown	unknown	gnl\|CDD\|236410
NZ_CP054599.1\|WP_037922468.1\|157021_157873_-\|peptidylprolyl-isomerase	unknown	unknown	gnl\|CDD\|234605
NZ_CP054599.1\|WP_037922470.1\|155792_157019_-\|bifunctional-glutamate-N-acetyltransferase/amino-acid-acetyltransferase-ArgJ	unknown	unknown	gnl\|CDD\|235441
NZ_CP054599.1\|WP_037922444.1\|173641_174535_+\|UTP--glucose-1-phosphate-uridylyltransferase-GalU	unknown	unknown	gnl\|CDD\|224131
NZ_CP054599.1\|WP_037922454.1\|168520_169123_+\|alpha-ketoglutarate-dependent-dioxygenase-AlkB	unknown	unknown	gnl\|CDD\|372627

Protein	Function_ID	Function_description	E-value
NZ_CP054599.1\|WP_037922476.1\|152565_155058_+\|translation-initiation-factor-IF-2	gnl\|CDD\|235401	PRK05306, infB, translation initiation factor IF-2; Validated.	0
NZ_CP054599.1\|WP_037922473.1\|155392_155791_-\|(deoxy)nucleoside-triphosphate-pyrophosphohydrolase	gnl\|CDD\|239517	cd03425, MutT_pyrophosphohydrolase, The MutT pyrophosphohydrolase is a prototypical Nudix hydrolase that catalyzes the hydrolysis of nucleoside and deoxynucleoside triphosphates (NTPs and dNTPs) by substitution at a beta-phosphorus to yield a nucleotide monophosphate (NMP) and inorganic pyrophosphate (PPi). This enzyme requires two divalent cations for activity; one coordinates the phosphoryl groups of the NTP/dNTP substrate, and the other coordinates to the enzyme. It also contains the Nudix motif, a highly conserved 23-residue block (GX5EX7REUXEEXGU, where U = I, L or V), that functions as metal binding and catalytic site. MutT pyrophosphohydrolase is important in preventing errors in DNA replication by hydrolyzing mutagenic nucleotides such as 8-oxo-dGTP (a product of oxidative damage), which can mispair with template adenine during DNA replication, to guanine nucleotides.	2.24985e-57
NZ_CP054599.1\|WP_037922466.1\|158068_160777_+\|preprotein-translocase-subunit-SecA	gnl\|CDD\|237259	PRK12904, PRK12904, preprotein translocase subunit SecA; Reviewed.	0
NZ_CP054599.1\|WP_037922448.1\|171458_172262_+\|3-deoxy-manno-octulosonate-cytidylyltransferase	gnl\|CDD\|235473	PRK05450, PRK05450, 3-deoxy-manno-octulosonate cytidylyltransferase; Provisional.	8.83422e-109
NZ_CP054599.1\|WP_037922450.1\|170661_171459_+\|3'(2'),5'-bisphosphate-nucleotidase-CysQ	gnl\|CDD\|238816	cd01638, CysQ, CysQ, a 3'-Phosphoadenosine-5'-phosphosulfate (PAPS) 3'-phosphatase, is a bacterial member of the inositol monophosphatase family. It has been proposed that CysQ helps control intracellular levels of PAPS, which is an intermediate in cysteine biosynthesis (a principal route of sulfur assimilation).	3.0837e-102
NZ_CP054599.1\|WP_037922442.1\|174554_175541_+\|UDP-glucose-4-epimerase-GalE	gnl\|CDD\|224012	COG1087, GalE, UDP-glucose 4-epimerase [Cell envelope biogenesis, outer membrane].	3.41226e-176
NZ_CP054599.1\|WP_037922462.1\|161909_163469_+\|substrate-binding-domain-containing-protein	gnl\|CDD\|270371	cd13653, PBP2_phosphate_like_1, Substrate binding domain of putative ABC-type phosphate transporter, a member of the type 2 periplasmic binding fold superfamily. This subfamily contains uncharacterized phosphate binding domains found in PstS proteins that serve as initial receptors in the ABC transport of phosphate in eubacteria and archaea. After binding the ligand, PstS interacts with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis. The PstS proteins belong to the PBP2 superfamily of periplasmic binding proteins that differ in size and ligand specificity, but have similar tertiary structures consisting of two globular subdomains connected by a flexible hinge. They have been shown to bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap.	5.35248e-48
NZ_CP054599.1\|WP_037922456.1\|166421_168332_-\|molecular-chaperone-DnaK	gnl\|CDD\|234715	PRK00290, dnaK, molecular chaperone DnaK; Provisional.	0
NZ_CP054599.1\|WP_037922477.1\|151933_152530_+\|RNA-binding-protein	gnl\|CDD\|236401	PRK09190, PRK09190, RNA-binding protein.	2.73875e-90
NZ_CP054599.1\|WP_081855332.1\|175620_176733_+\|glycosyltransferase	gnl\|CDD\|340838	cd03809, GT4_MtfB-like, glycosyltransferases MtfB, WbpX, and similar proteins. This family is most closely related to the GT4 family of glycosyltransferases. MtfB (mannosyltransferase B) in E. coli has been shown to direct the growth of the O9-specific polysaccharide chain. It transfers two mannoses into the position 3 of the previously synthesized polysaccharide.	1.53159e-41
NZ_CP054599.1\|WP_143186733.1\|172159_173491_+\|glycosyltransferase	gnl\|CDD\|133029	cd04186, GT_2_like_c, Subfamily of Glycosyltransferase Family GT2 of unknown function. GT-2 includes diverse families of glycosyltransferases with a common GT-A type structural fold, which has two tightly associated beta/alpha/beta domains that tend to form a continuous central sheet of at least eight beta-strands. These are enzymes that catalyze the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. Glycosyltransferases have been classified into more than 90 distinct sequence based families.	5.3149e-25
NZ_CP054599.1\|WP_037922452.1\|169718_170543_-\|ABC-transporter-permease	gnl\|CDD\|185098	PRK15176, PRK15176, Vi polysaccharide ABC transporter inner membrane protein VexB.	4.64565e-12
NZ_CP054599.1\|WP_037922454.1\|168520_169123_+\|alpha-ketoglutarate-dependent-dioxygenase-AlkB	gnl\|CDD\|372627	pfam13532, 2OG-FeII_Oxy_2, 2OG-Fe(II) oxygenase superfamily.	4.58761e-67
NZ_CP054599.1\|WP_051693970.1\|164294_165104_-\|methyltransferase-domain-containing-protein	gnl\|CDD\|369777	pfam08241, Methyltransf_11, Methyltransferase domain. Members of this family are SAM dependent methyltransferases.	4.17408e-30
NZ_CP054599.1\|WP_037922480.1\|150289_151912_+\|transcription-termination/antitermination-protein-NusA	gnl\|CDD\|236410	PRK09202, nusA, transcription elongation factor NusA; Validated.	0
NZ_CP054599.1\|WP_037922468.1\|157021_157873_-\|peptidylprolyl-isomerase	gnl\|CDD\|234605	PRK00059, prsA, peptidylprolyl isomerase; Provisional.	1.29496e-26
NZ_CP054599.1\|WP_037922470.1\|155792_157019_-\|bifunctional-glutamate-N-acetyltransferase/amino-acid-acetyltransferase-ArgJ	gnl\|CDD\|235441	PRK05388, argJ, bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ.	0
NZ_CP054599.1\|WP_037922444.1\|173641_174535_+\|UTP--glucose-1-phosphate-uridylyltransferase-GalU	gnl\|CDD\|224131	COG1210, GalU, UDP-glucose pyrophosphorylase [Cell envelope biogenesis, outer membrane].	2.28779e-152
NZ_CP054599.1\|WP_051693968.1\|169119_169722_-\|hypothetical-protein	gnl\|CDD\|377965	pfam08239, SH3_3, Bacterial SH3 domain.	9.65974e-06

>NZ_CP054599.1|WP_051693970.1|164294_165104_-|methyltransferase-domain-containing-protein
MDYSEFRQDEINGWSQRAGGYGKATALATVQTIPTLLAAVRLYPGARMLDAGCGPGYAAGAAMALGASCVGVDNAPAMIDAAHTRYPGSEFKTGDIEALDFPDHSFDIVAANIVLFHVTDPRKSIAEAFRVLRPGGRFAFSQWCGPDRSECYKLIYDVISAHADMTLASPAPNAFDLSQPDHAKSVLEDAGFTDTATVHVPNVLRTGAESFYDFFMQFGVRIPLIMQRQSNQVQATVRAEIDERAQAFRVGDEFHMPMPSIVVSGQRPA
>NZ_CP054599.1|WP_037922462.1|161909_163469_+|substrate-binding-domain-containing-protein
MVLKRAAALAALFLFGFVAPVAAQDVTLTSRDGAIELSGMLLGFDGEFYRLETEFGELTVDSSGVLCEGPACPNLQDYVAELSVSGSTTMGAVLMPALVEGFAQRAGYDAVREPVDDTRFRYLLTNTETGKLAARFHFRVTTTDEGFADLLANEADIVMALREIRPGERQRAREAGMGDMTGLHRSRVLALDAVVPVVAPGNPVRSISPLDLARVFSGQVTNWQDLGGVDAPISLHLPQAATGLSQAVADQVMKPVRLEITDNVVRHPLGTALSAAVADDPFAIGIASYAEVGNAVPLVLTGACGFALEAGRRTIKTEDYPLTAPMFLYLPARRLPQIAREFLAFTRSPTAQVVIRRAGFVDQSPEEVPIDMQGNRFANAISTAGPEVPLEELQRMVRTLDGMSRLTTSFRFEAGSIRLDAQSRSNVQQLARAMEVGTYDARRILFVGFSDGDGPSAANRNIALRRADAVRKAITEAAQSEDTKRVTLDVDAFGEALPMACDDSAWGRQANRRVEVWVR
>NZ_CP054599.1|WP_037922464.1|160895_161888_+|hypothetical-protein
MAYYKEIATAAGTLVCAVGIGFIMQSSDTAESRYGADTAVGSQSAAQAPAAAADTSALDGPVLDVHDIVLTSALEDAPGMRDTVVALAPADTLVTPDTTDVAPGQSCTVNATAKPSGLGLVDLTVTAPCFANERLTVHHSGMRFTETTDATGALDLSMPVLADPAVIVVAFANGDGAVAQTAVPSLGDFDRVVLQWKGDAGFQIHAREFGADYGTEGDIWAESPGDPVQAISGEGGFLVRLGDPSASEAMVADIYTYPSGASRHAGVVDLSAEVEVSLFNCGLEIEAQSLELRDSEIMTRNLNMTVPGCDTIGSFLVLNNLIQDLTVAQK
>NZ_CP054599.1|WP_037922466.1|158068_160777_+|preprotein-translocase-subunit-SecA
MLGIGTLAKKVFGTPNDRLVKSVRPLVEKINALEPEFEALSDAGLKDKTEEFAKRALQGESLDDLLPEAFANCREAARRALGLRAFDTQLMGAIFMHRGNIAEQKTGEGKTLTATFAAYLNALTGKGVHVVTVNEYLAKRDAEWMGKVFAHLGLTSGCAYSGMGEDKKRAAYASDVTYATNNELGFDYLRDNMKSELGQMVQRGHNFAIVDEVDSILIDEARTPLIISGPLDDRSELYQTIDAVIPLLDESHYSVDEKTRNVSFTEEGNEFLEQQLLARGLIEEGMTLYDPESTSIVHHVNQGLRAHKLFQKDKDYIVRNDEVVLIDEFTGRMMAGRRLSEGLHQAIEAKEGVNIQPENVTLASVTFQNYFRLYDKLAGMTGTALTEAEEFNEIYGLGVVEVPTNRPIARVDEDDRVYRTATEKYAAMIEEIKEAHEKGQPVLVGTTSIEKSEMFSAILTEKGIPHNVLNARQHEQEAQIIADAGKFGAVTIATNMAGRGTDIQLGGNVDLKVLEALTADPDADPEAIRARIEAEHEEEKQKVLAAGGLFVLASERHESRRIDNQLRGRSGRQGDPGRTSFYLSLEDDLMRIFGSDRLDKVLKTLGMEEGEAIIHPWVNKSLERAQAKVEGRNFDMRKQLLKFDDVMNDQRKVVFSQRRDIMEAEDLSEITSDMREQVIDDLIDQYMPQGSYADQWNTEGLYAAVMENLGVDVPVMAWAEEDGVDDEQIRERLIEATDKLMAQKAEVFGPENMRMIEKQILLQAIDGKWREHLLTLDHLRSVVGFRGYAQRDPLNEYKNEAFQLFEVMLDSLREDVTQKLAQIRPMTEEERTEMLEQAAAQQAQLQQAAKDSRDAQTPAPSAEAVATGFDENDPTTWGNPGRNEPCPCGSGKKFKHCHGTLT
>NZ_CP054599.1|WP_037922468.1|157021_157873_-|peptidylprolyl-isomerase
MLKRLSILPAVAVALTLSQPLYAQDETPDLSTVVATVDGTEITLGHMLVAKATLPQQYQQLPDEVLFPGILDQLIQQTALEKSYEGDLPKRVELSMENERRSLIAGEAIEKILATAVSEEAIQAAYDATYANAEPTQEFNASHILVETEDEAKAIIEELNGGADFAATAREKSTGPSGPGGGSLGWFGPGAMVPEFETAVAAMKAGDISEPVQTQFGWHVIKLNEVRNAEAPALEDVRAELEQQVRSEAVTAAVDALVADADVNRDGAEGMDPSVLSTLKLGE
>NZ_CP054599.1|WP_037922470.1|155792_157019_-|bifunctional-glutamate-N-acetyltransferase/amino-acid-acetyltransferase-ArgJ
MAGKIALSPLAPERFPDLPVVDGVTFATIAAGVRYAGRTDVMLAKMVPGTAIAGVFTRSSTRAAPVLDCQEKLGGDPAGGAAFLVNSGNANAFTGKKGAESVHEIVEAVSINCGVPSARVFTSSTGVIGEPLPHDRVVAKMGELNDALRDDGIAEAARAIMTTDTYPKGAVETVEVNGETITIAGIAKGSGMIAPDMATMLVYIFTDARIAQDNLQAIVSTAADLTFNCITVDSDTSTSDSVLMAATGASGVDVTGDASFADAVHRLMQNLAFQVVRDGEGATKFVEITVSGADTAANAKVHAMAIANSPLVKTAIAGQDPNWGRVVMAIGKSGAPADRDLLSIRFGDVIVAENGWVSPQYSEADAAAHMKGDEINITVDLGLGEAAATVWTCDLTHAYIDINADYRS
>NZ_CP054599.1|WP_037922473.1|155392_155791_-|(deoxy)nucleoside-triphosphate-pyrophosphohydrolase
MKVVLVSAVALIDVDGRVLLAQRPEGKSMAGLWEFPGGKVEPGETPEAALIRELHEELGIETWQSCLAPLTFASHSYDDFHLLMPLFACRKWGGTPMSKENQALKWVRPNDLKTYPMPAADVPLIPILRDWL
>NZ_CP054599.1|WP_037922476.1|152565_155058_+|translation-initiation-factor-IF-2
MSDTDGKKTLGLRGGARPGNVKQSFSHGRTKNVVVETKRKRVVVPKTGAAKGGTSNPSLGDPAKRPAGITDAEMERRLKAVQAARSREVEEAAAREAEEKARAEERERRRAEQEAKEQEEREREESLKAKAEEEERKAREAAAAAAQAAAAPAPTDVDQPQRAAPSRAAPQQDANAARKKDRDADQRTARNKGADDGRRSGKLTLNQALSGGEGGRQRSMAAMKRKQERARQKAMGGSVEREKVTRTVNLPEAIVVSELANRMAERVGDVVKSLMQMGMMVTQNQTIDADTAELIIEEFGHTVNRVSDADVEQVINEVEDKPEDLEARPPVITIMGHVDHGKTSLLDAIRNAKVVAGEAGGITQHIGAYQVTTDSGQLLTFLDTPGHAAFTSMRSRGAQVTDIVVLVVAADDAVMPQTVEAINHAKAAKVPMIVAINKIDKPGANPDKVRTDLLQHEVIVEKMSGEVQDVEVSAIKGEGLDDLLEAIALQSELLELKANPNRAAQGAVIEAQLDVGRGPVATVLIQNGTLRQGDIFVVGEQYGKVRALINDKGERIKEAGPSVPVEVLGLNGTPEAGDVLNVTSTEAQAREIAEYREKAAKDKRAAAGAATTLEQLMANAKENENVSELPILVKADVQGSAEAIVQAMEKIGNDEVRVRVLHSGVGAITETDVGLAEASGAPIMGFNVRANASARNTANQKGVEIRYYSVIYDLVDDVKAAASGLLSNEIKENFIGYANIKEVFKVTGVGKVAGCLVTEGVARRSAGVRLLRDNVVIHEGTLKTLKRFKDEVSDVQSGQECGMAFENYDDIREGDVIEIFEREEVTRTLA
>NZ_CP054599.1|WP_037922477.1|151933_152530_+|RNA-binding-protein
MARGGATKDREDGPERKCIATGEVQPKYGLVRFVVGPDTQIVPDIAGKLPGRGIYVAADRDAIQKAVTKKLFSRGAKMQVTVQEDLVDEVERQLARRVVDLIALSRKSGRAVAGYEKVKTWLQTEEAEVLIQAVDGSARGKTKLSTPHYGSYIGWLSADELGLAFGRQTVIHGALASGGLTQRVVEEARRLKGLRPNA
>NZ_CP054599.1|WP_037922480.1|150289_151912_+|transcription-termination/antitermination-protein-NusA
MAITSANQLELLQTAEAVAREKMIDPGLVIEAMEESLARAAKSRYGAEMDIRVSIDRKTGRATFTRVRTVVAEDELENYQAEFTVEQAKQYMADPKIGDTFVEEVPPVEMGRIAAQSAKQVILQKVREAERDRQYEEFKDRAGTIINGLVKREEYGNVIVDVGAGEAILRRNEKIGRESYRPNDRIRVYIKDVRREQRGPQIFLSRTAPEFMAELFKMEVPEIYDGIIEIKAVARDPGSRAKIAVISYDNSIDPVGACVGMRGSRVQAVVNELQGEKIDIIPWNDDQPTFLVNALQPAEVTKVVLDEEAGKIEVVVPEEQLSLAIGRRGQNVRLASQLTGLDIDIMTEAEESARRQAEFEVRTKLFMDNLDLDEFFAQLLVAEGFTSLEEVAYVEQDELLVIDGVDSDTAKELQARAQDVLAEQNKAALEAARALGVEDSLVEFEGLTPQMIEALAKDDVKTLEDFATCADWELAGGWTTVNGERVKDDGVLEPFDISLEEAQTMIMTARVLLGWVDPTELEADEDLDDEDGAESEEAEA
>NZ_CP054599.1|WP_037922456.1|166421_168332_-|molecular-chaperone-DnaK
MAKVIGIDLGTTNSCIAIMDGSQPRVIENAEGARTTPSIVAFTDDERLVGQPAKRQAVTNPDNTIFGVKRLIGRRNDDPDLAKDKKNLPFTVIDGGNGDAWVQAKGEKYSPSQISAFILGKMKETAESYLGEEVTQAVITVPAYFNDAQRQATKDAGKIAGLEVLRIINEPTAAALAYGLDKENTQTIAVYDLGGGTFDVTILEIDDGLFEVKSTNGDTFLGGEDFDMRIVNYLAGEFKKEHNVDLTQDKMALQRLKEAAEKAKIELSSSSQTEINQPFISMGSNGQPLHMVMKLTRAKLESLVSDLIKASMKPCAAALKDAGLSASDIDEVVLVGGMTRMPRVIEEVTKFFGKEPHKGVNPDEVVALGAAIQAGVLQGDVKDVVLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQVFSTAEDNQNAVTIRVFQGEREMAADNKILGAFNLENIPPAPRGMPQIEVTFDIDANGIVSVGALDKGTGKEQKITIQASGGLSDEDIEKMVKDAEENAEADKERRSLIEAKNQAESLIHSTEKSVEEHSDKVDPTTVEAIELAIAALKDELETDNVDKIKSGIQNVTEAAMKLGEAIYKAAQEEGEDAPHAADEPRSDDDDIVDAEFEDLDDDKRA
>NZ_CP054599.1|WP_037922454.1|168520_169123_+|alpha-ketoglutarate-dependent-dioxygenase-AlkB
MTKLILRDVEIHKGFLDRTAQGELLDAVRGVVAAAPLFQPDTPYGKPMSVRMSSAGRYGWFSDRSGYRYADRHPSGAPWPPVPEAILAIWDQVTGLARRPDCCLINYYGEGARMGLHQDRDEADFAWPVVSVSLGDPGLFRVGNPTRGGKTESVWLESGDVVVLGGPARLVYHGVDRIKFGAGSLLPRGGRINLTLRVVD
>NZ_CP054599.1|WP_051693968.1|169119_169722_-|hypothetical-protein
MIRALAVSLALLCWVPQAGWAFDFPALYHVIDVADDDVLNVRAQPDAQSDIIGTLLHDEAGVEVIALSDDGKWGLINVGENAGWAAIRFLQHSGAPDRPLRAKCGGTEPFWNLSLGTTHVFRAPETGPQPYQPTAELSSPNTTNSLAILGRGPSGQMIATVDARACSDGMSDRNYGLSIGLVLTDEHGARYLTGCCSLSP
>NZ_CP054599.1|WP_037922452.1|169718_170543_-|ABC-transporter-permease
MFQSTRKPQSGIGTAILIAELVYHSVVRNVRKTHNNAFMAIAMNMLQAAIFVLAFYVMFSILGLRGSALRGDFLVYIMSGIFLYMTHTKTLGAVVGSEGPASPMMQHAPMNTIIAIASAAVGTLYIQVLSLFMILFIYHVAFVPLEIDRPIAAFGMLLVAWFTGLALGLVLLSIKPWFPTFVNIFTTIYQRANMIASGKMFVANTLPGYMLAMFDWNPLFHCIDQARGFAFINYAPHHSSVSYPIYVGGVLIMLGLMGEFYTRRHASLSWNARR
>NZ_CP054599.1|WP_037922450.1|170661_171459_+|3'(2'),5'-bisphosphate-nucleotidase-CysQ
MNYETLVPVMRRLALEAGDKIMEIYDADDFEVKTKSDDSPVTAADEAADAIISEGLREAFPDLMLVTEEQSDTHDTTGDTFLIVDPLDGTKEFVHRRGDFTVNIALVEAGKPTRGVVYAPAKNRMFFTQADGQTVEEAGPFQKDLIGSLTPLRVSQPENDALMVVASKSHRDQATDDYINKYAVKDMTSAGSSLKFCLVATGEADLYPRLGRTMEWDTAAGHAVLLGAGGKVVRFDDHSDLTYGKDDYANPFFIAYSPDVALKPA
>NZ_CP054599.1|WP_037922448.1|171458_172262_+|3-deoxy-manno-octulosonate-cytidylyltransferase
MSVLVVIPARYASTRYPGKPLVALTGASGVASTLIERSWRAAMSVQGVDRVVVATDDDRIADAARAFGAEVVMTSTQCANGTERCAEAHAALGGGYDVVVNLQGDAPLTPPWFVEALIEAMQASQDADIATPVLRCDGATLNGLLADRKAGRVGGTTAVFDRNRKALYFSKEVIPYIGTTFAEDADETPVFHHVGVYAYRPAALAAYPGWAVGPLEQLEGLEQLRFMESGGAVLCVEVDARGRQFWELNNPEDVPRIETMMSQMGLE
>NZ_CP054599.1|WP_143186733.1|172159_173491_+|glycosyltransferase
MRRSGRAWPSVLGAEQSRRRAPDRDDDVPDGSGMTQPPVSVVIVSRGRPDALRVCLTGCMQLLYDPFEIVVVADPVGVAAAQALSFAKQIKIIGFDDANISAARNVGINAAAGEIVAFVDDDAVPEPTWLTHLTQAMQQHDVVVAGGYVRGRNGISYQWKGRTLDALGDAVDMPVTGLEPVVVQPTPTRCAKTEGTNMAVRRDVLVEVGGFDPAYHYFLDETDLNMRLAKAGYACAIVPRAQVHHGFAANKLRRADRVPTDLFDIGASWAVFQRKFIPQDKRRDHWRTIRMSERRRAVRLMVSGHIEPRAVTGLISRLDQGYGQGIARRMGEGHVTQTPNAPFKRVAPLSGSMSYMAAWRSNAAKARAEALRRVKNGEVVTLIILSPDALYHQARFHPDGYWEQRGGLFGKSSRSQSLLKFWRFNDRFSAEKKRLSAIRGGPD
>NZ_CP054599.1|WP_037922444.1|173641_174535_+|UTP--glucose-1-phosphate-uridylyltransferase-GalU
MPKKVTKAIFPVAGLGTRFLPATKSVPKEIMTLVDRPLIQYAIDEARAAGIKEFIFVTSRGKGALEDYFDHAPQLEQELRKKGKTELLDILKDTNMDSGAIAYIRQHKALGLGHAVWCARRLVGDEPFAVILPDDVIAAEKPCLQQMVEAYAETGGSMVAAMEVPPEKASSYGVLEVSEDMGDLVKVKSMVEKPAPGTAPSNLAVIGRYILSPNVLSNLNRMKSGAGGEIQLTDAIADEIAQDNPVYGYRFSGQRFDCGSKSGFLQATVSFGLAREELRDDLSAYIRDIVNMEKAAE
>NZ_CP054599.1|WP_037922442.1|174554_175541_+|UDP-glucose-4-epimerase-GalE
MDNHVLVTGGAGYIGAHACKALRRAGYVPVTFDNLVTGWQDAVKFGPFERGDLLDRARLDEVFAKYTPVAVMHFAALSQVGESMSQPGRYWHNNVGGSLNLVEAAVDAACMNFVFSSTCATYGDQDNVVLDENSAQYPINAYGASKRAIEDILRDFEAAHGLRNVIFRYFNVAGADPEAEVGEFHQPETHLIPLMLDAIDGKRDALTIFGTDYDTPDGTCIRDYVHVCDLVDAHVLGLKWLQDGKGSRVFNLGTGSGFSVREVIDQSRAVTNRTVPFKEGARRAGDATKLVSGSTRAVAELGWQPDRSNLNTMIADAWRWHQTGHFDK
>NZ_CP054599.1|WP_081855332.1|175620_176733_+|glycosyltransferase
MAYLRRLVADDVPAFGLVRTPLGYVLLDRAGMASLVLRIEGAVPWARPDLLSRLARGQTAVQQAAQTEVRRLAMARTLPRGLAAMLRKHLPQGTTYLNTGHSNLTDRVLQAAQGLGGRIGVFVHDVIPLEFPQFQRPETVEPFRAKMHRVQACADVIICNSADTLHRAGQVMAQWGKVPEGIVAHLGTDLTTPQPDLLPDDLPPSGPYFISVGTIEPRKNHALLLDVWDVMGADAPTLLICGARGWNNAAVFARLDGRTDTRVIERAGLSDGAVAALIAGSCGLVFPSFAEGYGLPPVEAAALGVPVVANDLPVIREILGNIPVYLPAHDRYLWEKTLKDMAEAGQLGREKVQFQAPAWSQHFNTVLRSL

You can click texts colored in the table to view more detailed information

Click the colored protein region to show detailed information

Crispr_ID: NZ_CP054599_2

CRISPR_ID

CRISPR_location

CRISPR_type

Repeat_type

Spacer_info

Cas_protein_info

CRISPR-Cas_info

NZ_CP054599_2

1970998-1971125

Orphan

Consensus_repeat	Method
ATCGGCAACAACAGCCTGATCGG	CRISPRCasFinder

2 spacers

The CRISPR arrays of NZ_CP054599_2

>merge|NZ_CP054599|2|1970998-1971125|CRISPRCasFinder
ATCGGTGAAAACTGCACCATCGGTCACAAGGTCATGCTGCACGGCTGCACCATCGGCAACAACAGCCTGATCGGCATGGGTGCCACGATCCTGAACGGGGCCAAGATCGGCAACAACTGCCTGATCGG

>NZ_CP054599|2|2|1970998-1971125|CRISPRCasFinder
ATCGGTGAAAACTGCACCATCGG	TCACAAGGTCATGCTGCACGGCTGCACC
ATCGGCAACAACAGCCTGATCGG	CATGGGTGCCACGATCCTGAACGGGGCCAAG
ATCGGCAACAACTGCCTGATCGG

Protein	Signature genes	Signature genes Name	Protein_function
NZ_CP054599.1\|WP_037920432.1\|1977937_1978732_+\|phosphate-ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|237645
NZ_CP054599.1\|WP_037920438.1\|1980976_1981774_-\|ABC-transporter-permease	unknown	unknown	gnl\|CDD\|224098
NZ_CP054599.1\|WP_037920392.1\|1960349_1961360_-\|branched-chain-amino-acid-ABC-transporter-permease	unknown	unknown	gnl\|CDD\|119324
NZ_CP054599.1\|WP_037920435.1\|1979464_1980154_+\|phosphate-regulon-transcriptional-regulator-PhoB	unknown	unknown	gnl\|CDD\|131209
NZ_CP054599.1\|WP_037920413.1\|1970122_1970782_+\|guanylate-kinase	unknown	unknown	gnl\|CDD\|234719
NZ_CP054599.1\|WP_037920433.1\|1978744_1979452_+\|phosphate-signaling-complex-protein-PhoU	unknown	unknown	gnl\|CDD\|273990
NZ_CP054599.1\|WP_037920427.1\|1972497_1973580_+\|PAS-domain-containing-protein	unknown	unknown	gnl\|CDD\|274368
NZ_CP054599.1\|WP_037920402.1\|1965636_1966641_-\|GlxA-family-transcriptional-regulator	unknown	unknown	gnl\|CDD\|227311
NZ_CP054599.1\|WP_037920394.1\|1962253_1963036_-\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|213186
NZ_CP054599.1\|WP_037920430.1\|1976575_1977919_+\|phosphate-ABC-transporter-permease-PstA	unknown	unknown	gnl\|CDD\|223654
NZ_CP054599.1\|WP_073194123.1\|1961432_1962257_-\|ABC-transporter-ATP-binding-protein	unknown	unknown	gnl\|CDD\|213191
NZ_CP054599.1\|WP_037921943.1\|1975112_1976576_+\|phosphate-ABC-transporter-permease-subunit-PstC	unknown	unknown	gnl\|CDD\|223646
NZ_CP054599.1\|WP_037920408.1\|1968292_1968994_-\|PAS-domain-containing-protein	unknown	unknown	gnl\|CDD\|284676
NZ_CP054599.1\|WP_037920406.1\|1966855_1968229_+\|3-deoxy-7-phosphoheptulonate-synthase-class-II	unknown	unknown	gnl\|CDD\|366662
NZ_CP054599.1\|WP_081855282.1\|1969237_1970116_+\|YicC-family-protein	unknown	unknown	gnl\|CDD\|236993
NZ_CP054599.1\|WP_037920423.1\|1971304_1972492_+\|aminotransferase-class-I/II-fold-pyridoxal-phosphate-dependent-enzyme	unknown	unknown	gnl\|CDD\|99738
NZ_CP054599.1\|WP_037921940.1\|1973895_1974936_+\|substrate-binding-domain-containing-protein	unknown	unknown	gnl\|CDD\|270372
NZ_CP054599.1\|WP_037920397.1\|1963142_1964339_-\|ABC-transporter-substrate-binding-protein	unknown	unknown	gnl\|CDD\|380569
NZ_CP054599.1\|WP_037920400.1\|1964520_1965636_+\|PQQ-dependent-sugar-dehydrogenase	unknown	unknown	gnl\|CDD\|369642
NZ_CP054599.1\|WP_051693767.1\|1980162_1980972_-\|alpha/beta-hydrolase	unknown	unknown	gnl\|CDD\|223730

Protein	Function_ID	Function_description	E-value
NZ_CP054599.1\|WP_037920432.1\|1977937_1978732_+\|phosphate-ABC-transporter-ATP-binding-protein	gnl\|CDD\|237645	PRK14235, PRK14235, phosphate transporter ATP-binding protein; Provisional.	0
NZ_CP054599.1\|WP_037920438.1\|1980976_1981774_-\|ABC-transporter-permease	gnl\|CDD\|224098	COG1177, PotC, ABC-type spermidine/putrescine transport system, permease component II [Amino acid transport and metabolism].	7.03294e-56
NZ_CP054599.1\|WP_037920392.1\|1960349_1961360_-\|branched-chain-amino-acid-ABC-transporter-permease	gnl\|CDD\|119324	cd06582, TM_PBP1_LivH_like, Transmembrane subunit (TM) of Escherichia coli LivH and related proteins. LivH is one of two TMs of the E. coli LIV-1/LS transporter, a Periplasmic Binding Protein (PBP)-dependent ATP-Binding Cassette (ABC) transporter involved in the uptake of branched-chain amino acids (AAs). These types of transporters generally bind type 1 PBPs. PBP-dependent ABC transporters consist of a PBP, two TMs, and two cytoplasmic ABCs, and are mainly involved in importing solutes from the environment. The solute is captured by the PBP, which delivers it to a gated translocation pathway formed by the two TMs. The two ABCs bind and hydrolyze ATP and drive the transport reaction. E. coli LivH forms a heterodimer with another TM, LivM, to generate the transmembrane pore. LivM is not included in this subgroup. The LIV-1/LS transporter is comprised of two TMs (LivM and LivH), two ABCs (LivG and LivF), and one of two alternative PBPs, LivJ (LIV-BP) or LivK (LS-BP). In addition to transporting branched-chain AAs including leucine, isoleucine and valine, the E. coli LIV-1/LS transporter is involved in the uptake of the aromatic AA, phenylalanine.	3.3137e-58
NZ_CP054599.1\|WP_037920435.1\|1979464_1980154_+\|phosphate-regulon-transcriptional-regulator-PhoB	gnl\|CDD\|131209	TIGR02154, PhoB, phosphate regulon transcriptional regulatory protein PhoB. PhoB is a DNA-binding response regulator protein acting with PhoR in a 2-component system responding to phosphate ion. PhoB acts as a positive regulator of gene expression for phosphate-related genes such as phoA, phoS, phoE and ugpAB as well as itself. It is often found proximal to genes for the high-affinity phosphate ABC transporter (pstSCAB; GenProp0190) and presumably regulates these as well. [Regulatory functions, DNA interactions, Signal transduction, Two-component systems].	1.07925e-123
NZ_CP054599.1\|WP_037920413.1\|1970122_1970782_+\|guanylate-kinase	gnl\|CDD\|234719	PRK00300, gmk, guanylate kinase; Provisional.	5.786e-109
NZ_CP054599.1\|WP_037920433.1\|1978744_1979452_+\|phosphate-signaling-complex-protein-PhoU	gnl\|CDD\|273990	TIGR02135, Uncharacterized_protein, phosphate transport system regulatory protein PhoU. This model describes PhoU, a regulatory protein of unknown mechanism for high-affinity phosphate ABC transporter systems. The protein consists of two copies of the domain described by pfam01895. Deletion of PhoU activates constitutive expression of the phosphate ABC transporter and allows phosphate transport, but causes a growth defect and so likely has some second function. [Regulatory functions, Other, Transport and binding proteins, Anions].	1.35334e-68
NZ_CP054599.1\|WP_037920427.1\|1972497_1973580_+\|PAS-domain-containing-protein	gnl\|CDD\|274368	TIGR02966, Phosphate_regulon_sensor_protein_PhoR, phosphate regulon sensor kinase PhoR. Members of this protein family are the regulatory histidine kinase PhoR associated with the phosphate ABC transporter in most Proteobacteria. Related proteins from Gram-positive organisms are not included in this model. The phoR gene usually is adjacent to the response regulator phoB gene (TIGR02154). [Signal transduction, Two-component systems].	8.63354e-104
NZ_CP054599.1\|WP_037920402.1\|1965636_1966641_-\|GlxA-family-transcriptional-regulator	gnl\|CDD\|227311	COG4977, COG4977, Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain [Transcription].	2.08418e-128
NZ_CP054599.1\|WP_037920394.1\|1962253_1963036_-\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|213186	cd03219, ABC_Mj1267_LivG_branched, ATP-binding cassette component of branched chain amino acids transport system. The Mj1267/LivG ABC transporter subfamily is involved in the transport of the hydrophobic amino acids leucine, isoleucine and valine. MJ1267 is a branched-chain amino acid transporter with 29% similarity to both the LivF and LivG components of the E. coli branched-chain amino acid transporter. MJ1267 contains an insertion from residues 114 to 123 characteristic of LivG (Leucine-Isoleucine-Valine) homologs. The branched-chain amino acid transporter from E. coli comprises a heterodimer of ABCs (LivF and LivG), a heterodimer of six-helix TM domains (LivM and LivH), and one of two alternative soluble periplasmic substrate binding proteins (LivK or LivJ).	5.77676e-104
NZ_CP054599.1\|WP_037920430.1\|1976575_1977919_+\|phosphate-ABC-transporter-permease-PstA	gnl\|CDD\|223654	COG0581, PstA, ABC-type phosphate transport system, permease component [Inorganic ion transport and metabolism].	1.89734e-85
NZ_CP054599.1\|WP_073194123.1\|1961432_1962257_-\|ABC-transporter-ATP-binding-protein	gnl\|CDD\|213191	cd03224, ABC_TM1139_LivF_branched, ATP-binding cassette domain of branched-chain amino acid transporter. LivF (TM1139) is part of the LIV-I bacterial ABC-type two-component transport system that imports neutral, branched-chain amino acids. The E. coli branched-chain amino acid transporter comprises a heterodimer of ABC transporters (LivF and LivG), a heterodimer of six-helix TM domains (LivM and LivH), and one of two alternative soluble periplasmic substrate binding proteins (LivK or LivJ). ABC transporters are a large family of proteins involved in the transport of a wide variety of different compounds, like sugars, ions, peptides, and more complex organic molecules.	1.86053e-107
NZ_CP054599.1\|WP_037921943.1\|1975112_1976576_+\|phosphate-ABC-transporter-permease-subunit-PstC	gnl\|CDD\|223646	COG0573, PstC, ABC-type phosphate transport system, permease component [Inorganic ion transport and metabolism].	1.51052e-93
NZ_CP054599.1\|WP_037920408.1\|1968292_1968994_-\|PAS-domain-containing-protein	gnl\|CDD\|284676	pfam07310, PAS_5, PAS domain. This family contains a number of hypothetical bacterial proteins of unknown function approximately 200 residues long. This region is is distantly similar to other PAS domains.	1.32708e-40
NZ_CP054599.1\|WP_037920406.1\|1966855_1968229_+\|3-deoxy-7-phosphoheptulonate-synthase-class-II	gnl\|CDD\|366662	pfam01474, DAHP_synth_2, Class-II DAHP synthetase family. Members of this family are aldolase enzymes that catalyze the first step of the shikimate pathway.	0
NZ_CP054599.1\|WP_081855282.1\|1969237_1970116_+\|YicC-family-protein	gnl\|CDD\|236993	PRK11820, PRK11820, YicC family protein.	7.48909e-103
NZ_CP054599.1\|WP_037920423.1\|1971304_1972492_+\|aminotransferase-class-I/II-fold-pyridoxal-phosphate-dependent-enzyme	gnl\|CDD\|99738	cd00614, CGS_like, CGS_like: Cystathionine gamma-synthase is a PLP dependent enzyme and catalyzes the committed step of methionine biosynthesis. This pathway is unique to microorganisms and plants, rendering the enzyme an attractive target for the development of antimicrobials and herbicides. This subgroup also includes cystathionine gamma-lyases (CGL), O-acetylhomoserine sulfhydrylases and O-acetylhomoserine thiol lyases. CGL's are very similar to CGS's. Members of this group are widely distributed among all three forms of life.	6.69171e-130
NZ_CP054599.1\|WP_037921940.1\|1973895_1974936_+\|substrate-binding-domain-containing-protein	gnl\|CDD\|270372	cd13654, PBP2_phosphate_like_2, Substrate binding domain of putative ABC-type phosphate transporter, a member of the type 2 periplasmic binding fold superfamily. This subfamily contains uncharacterized phosphate binding domains found in PstS proteins that serve as initial receptors in the ABC transport of phosphate in eubacteria and archaea. After binding the ligand, PstS interacts with a cognate membrane transport complex comprised of two integral membrane domains and two cytoplasmically located ATPase domains. This interaction triggers the ligand translocation across the cytoplasmic membrane energized by ATP hydrolysis. The PstS proteins belong to the PBP2 superfamily of periplasmic binding proteins that differ in size and ligand specificity, but have similar tertiary structures consisting of two globular subdomains connected by a flexible hinge. They have been shown to bind their ligand in the cleft between these domains in a manner resembling a Venus flytrap.	1.24202e-73
NZ_CP054599.1\|WP_037920397.1\|1963142_1964339_-\|ABC-transporter-substrate-binding-protein	gnl\|CDD\|380569	cd06346, PBP1_ABC_ligand_binding-like, type 1 periplasmic ligand-binding domain of uncharacterized ABC (Atpase Binding Cassette)-type active transport systems predicted to be involved in uptake of amino acids, peptides, or inorganic ions. This subgroup includes the type 1 periplasmic ligand-binding domain of uncharacterized ABC (Atpase Binding Cassette)-type active transport systems that are predicted to be involved in uptake of amino acids, peptides, or inorganic ions. This subgroup has high sequence similarity to members of the family of hydrophobic amino acid transporters (HAAT), such as leucine-isoleucine-valine binding protein (LIVBP); however, its ligand specificity has not been determined experimentally.	6.55474e-96
NZ_CP054599.1\|WP_037920400.1\|1964520_1965636_+\|PQQ-dependent-sugar-dehydrogenase	gnl\|CDD\|369642	pfam07995, GSDH, Glucose / Sorbosone dehydrogenase. Members of this family are glucose/sorbosone dehydrogenases that possess a beta-propeller fold.	5.4138e-170
NZ_CP054599.1\|WP_051693767.1\|1980162_1980972_-\|alpha/beta-hydrolase	gnl\|CDD\|223730	COG0657, Aes, Esterase/lipase [Lipid metabolism].	8.64751e-19

>NZ_CP054599.1|WP_037920413.1|1970122_1970782_+|guanylate-kinase
MTIERRKNDRRGLLMILSSPSGAGKSTLAKRLRAWDTTISFSVSATTRPPRVGEVDKFDYHFVTEDVFKTMVNNDEMLEHAHVFGNFYGSPTAPVRAAIEQGNDVLFDIDWQGAQQIRNSVLGMHTLSVFLLPPSIHELRRRLEARGQDSKEVIDQRMKKCWDEISHWDGYDYVLVNDDLDETEDSLKKIIEVARMRRSQQPNLTEHVRKLQSEFQEQI
>NZ_CP054599.1|WP_081855282.1|1969237_1970116_+|YicC-family-protein
MTAFATSAGESGAYRWSCELRSVNGKGLDIRLRVPDWVEGLEVALRALVAAHVARGNITLNVRVTREDAGGMVQVNNAQLSAVLTALTEIEAQAMDLGLSLAPSKATDIAVMRGVLDQTSVDQDTGPLKAAIEADVTALVQDFVQMRASEGRALAHVLHAQLDEVAALTAEAARVAEARKDEVAENLRRNLARIVENADGLDAARVAQELALLAVKSDITEEIDRLGAHVTAARDLIDAAGPVGRKLDFLMQEFNREANTLCSKAQNTELTRVGLALKALIDQMREQVQNVE
>NZ_CP054599.1|WP_037920408.1|1968292_1968994_-|PAS-domain-containing-protein
MDFFERNPQNVVAMTDFQADSGFSALSQVEGYWDALRRSRLMPKRSEIDPRGIESALEFTFILERIAPGMARLRIAGSHLGDLMGMEVRGMPLTALIAPDGRRQVCDALEEVFQRPAVCEVHLNAPAAFDKPALEARMVLLPLKSDLGDVSRALGCFVARGTIGRKPRRFDVISVTSRAITAPATTSAPTEPSPGQNPTRQSGMAEDAKPFVKGRRSGDVPYLRLIKTDTKTD
>NZ_CP054599.1|WP_037920406.1|1966855_1968229_+|3-deoxy-7-phosphoheptulonate-synthase-class-II
MSEWSKSDWRSKPRIQMPDYTDTAALEAVTAQLSKYPPLVFAGEARRLKAHLGAASRGEAFLLQGGDCAEAFDQFSADSIRDTFKVMLQMAMVLTFGAKVPVIKVGRMAGQFAKPRSAPTEVIDGVELPSYRGDIINGLEFTPEARIPDPAKMLQAYTQAAATLNLLRAFSTGGFADMNRVHSWTLGFTDTEDAAKYAEIANRIQDTIDFMGAAGINSKTTHEFSTVEFYTSHESLLLEYEEALTRIDTTSGKWLAGSGHMIWIGDRTRQPDGAHVEYARGVQNPIGLKCGPTTTADDLKVLMSKLNPENEAGRLTLIARFGAGSVGDHLPRLIKAVQEEGANVLWTCDAMHGNTIKSSSGYKTRPFESVLREVREFFAVHQAEGTVPGGVHFEMTGQDVTECTGGVRAVSDEDLSDRYHTACDPRLNASQSLELAFLVAEELTARRNTQISQQHVG
>NZ_CP054599.1|WP_037920402.1|1965636_1966641_-|GlxA-family-transcriptional-regulator
MSQTRSATNRSVSPDKPRRYVFVLLNNFTLLCFSAALESLRIANRMAGRDLYEWRIIGEGGETAVCSAGTAFKLDGDLEELNRDDTVMVCGGIDVQDATSKRLLGWLRREARKGLQMGGLCTAAYSLARAGLLDGKKATIHWENQDSFAEEFEEVNLTKSVFVVDGQRITTAGGTSSIDLMLKLIADDHGEDMANAVADQLIYSSIRTDQDTQRLSVPTRIGVRHPKLSQVIQIMETNVEEPISPSILAKDVGMSTRQLERLFRRYLSRSPKRYYMELRLQKARNLLMQTDMSVINVALACGFASPSHFSKCYRAHYDTTPYRERGSHAAKLSI
>NZ_CP054599.1|WP_037920400.1|1964520_1965636_+|PQQ-dependent-sugar-dehydrogenase
MMRLFTILCVLWGFGTVSGVPQAQAQETSAGPVTFSAQVQDLDTPWAFDFLPEGGILVTERAGTLWHVRPDGSRVAVTGVPEVAASGQGGLLDVTLPRDFADSRTVLLTYAKPQDGGGAGTALAAGVLSADGSSLGDVQVLFEMKPGNQGGRHFGSRVVEGADGTLFVTMGDRGKGPLAQDLSQHNGKVVRVMRDGSVPSDNPFAAQSDALPDIWSFGHRNTQGAALDADGRLWVVEHGAKGGDEVNLTVKGVNYGWPVISYGRNYNGTKIGEGTEKDGMQQPAHYWDPSIAPSGAMFYDGDMFPDWKGNLFVGALKFNYISRLQIDGDAAQEVEQIKAPETARVRDIQTAPDGSIWFLSVTDGTLYRMAL
>NZ_CP054599.1|WP_037920397.1|1963142_1964339_-|ABC-transporter-substrate-binding-protein
MKKFLMATAATALMTGTAFADAHAKEVKIGVILGFTGPLESITPAMGAGAELAMAEVTDSGALLDGAKVVSIRGDSTCIDAGAATSAAERLITSDKVSAIMGADCSGVTGAILQNAARPNGIVMISPSATSPGLSTAEDDGLFFRTAPSDARQGVIMTEILQDRGVKEVALTYTNNDYGKGLADAFQAAFEEAGGTVTIATAHEDGKADYSAEVGALASAGGEALVVAGYVDQGGSGIVRSSIDSGAFDTFYFPDGMVGEKLNENFGAELNGSFGAYPGTDSEGAAKFLELATAAGFDGTGAFAPESYDAAALIMLAMQAANSMDSADFKDKVMDVANAPGEKIYPGELAKALELIKNGEDVDYEGASAVELIGPGESAGNYQEIEFADGKYSTIKYR
>NZ_CP054599.1|WP_037920394.1|1962253_1963036_-|ABC-transporter-ATP-binding-protein
MIVVDDIHKHFGGFHAVDGASLSIKPGSITGLIGPNGAGKTTLFNVIAGVLKPTSGRVTMNGEDITGLPPHTLFHKGLLRTFQIAHEFSSMTCRENLMMVPGNQSGETLWNTWFGRKRIADEERALRAKADEVLEFLTVEHLADHKAGQISGGQKKLLELGRTMMVDAKIVFLDEVGAGVNRTLLNTIGDAILRLNQERNYTFVVIEHDMDFIGKICDPVICMAEGKVLAEGTLDEIKANEHVIEAYLGTGLKNKEQVGA
>NZ_CP054599.1|WP_073194123.1|1961432_1962257_-|ABC-transporter-ATP-binding-protein
MSGNPYSDGRGNKDASIANPKGVGTLSAHPGTGAAMNTRNAFLIGDSMTGGYGSGPDILHDCTVAVDAGEIAVIVGPNGAGKSTAMKAVFGMLDVRQGSVRLDGEDITDLSPQDRVAKGMGFVPQTSNIFTSMTVEENLEMGAFIRRDDFRDTMAQVYDLFPILKEKRRQAAGELSGGQRQQVAVGRALMTRPKVLMLDEPTAGVSPIVMDELFDRIIEVARTGIPILMVEQNARQALEIADKGYVLVQGANAFTGTGKELLADPEVRKSFLGG
>NZ_CP054599.1|WP_037920392.1|1960349_1961360_-|branched-chain-amino-acid-ABC-transporter-permease
MDLLNAFVALSNFVLIPAIAYGSQLALGALGVTLIYGILRFSNFAHGDTMAFGTMVTILFTWLFQSWGISLGPVPTALLALPFGILGCMALLLFTDRVVYKFYREQKAKPVILVIVSLGVMFIMNGLVRFIIGPDDQRFADGERFIVSVRTFKDMTGLEEGLAIKTTQGITVITAVIVVAALFWFLNRTRTGKSMRAYSDNEDLALLSGINPERVVMITWMIVAALATVAGVLYGLDKSFKPFTYFQLLLPIFASAIVGGLGSPLGAIAGGFTIAFSEVMVTYAWKKVAGYALPESLAPDGLAQLLSTDYKFAVSFVILLIVLLFRPTGLFAGKSV
>NZ_CP054599.1|WP_037920423.1|1971304_1972492_+|aminotransferase-class-I/II-fold-pyridoxal-phosphate-dependent-enzyme
MRNEKGSLIRPVPLPGSASRPVVTPLSPSVVYASDTPDMLDGQYEGKLHGYTYSREGHPNADVVAGLLDRMENAPNPGVVTSSGMAAVSAVLLGLLKSGDHVIGGNQLYGRSLRMMAEDLPRLGIETTLADPGDVDAVRAALRPETRMILVEVVSNPTLRVADMKGLAKLAKEAGVLLVVDNTFTTPRAYQPFEHGADIVIHSVTKLLAGHSDVMLGYVAARDAAINTRLRVFTVTAGMTPSPFDCWLAERGMLSFDLRFERCQTTAMKLADALAGFSGVKRVIYPARTDHPDHARAADLLQGRWCNMVSFELDGGRDAANAFTTAAEGLSFAPTLGDVGTTLSHPASSSHRALTAERRASLGMSEGFFRVSVGLEDPDALVAIFERAVAAAVKG
>NZ_CP054599.1|WP_037920427.1|1972497_1973580_+|PAS-domain-containing-protein
MPQDHAIRAMLEALPMPGLVVNQSEVIVGANAAARNLIGQRIEGRPYVTMLRQPALLDAVEACLRQGKPRTAPYLTNDGSQHSTFSVHVAQVVVDDEAPMVLLTFEDVTQVQAAGQMRRDFVANVSHELRTPLTALMGFIETLRGPARNDPAAQARFLGIMQDQAERMNRLVGDLLSLSRVEGDERVRPTTQVNLRECLSSVMPLMTPLAEESSVTLVAELGDDPVAVAADRDQLVQVVSNLIENAIKYGGKNGKVTLRLEVHDYLRPLRGPGATIVVEDDGPGIDPVHLPRLTERFYRADNHRSRELGGTGLGLAIVKHIVSRHRGRLRIDSVLGQGTTVQVALPLLQPEGSGVIQDHP
>NZ_CP054599.1|WP_037921940.1|1973895_1974936_+|substrate-binding-domain-containing-protein
MSFVKLATSTFAIAAVAAGAAAARDQVQVAGSSTVLPYASIVAEAFGENFDFPTPVVESGGSSAGLKRFCEGVGENTIDVANASRKIKDSEVEACAANGVTDIIEVQIGYDGIVFASDINGNTFEFTPTDWYKALSDKVVVDGEVVANPYTTWDQVNPAFPAQPIQAFVPGTKHGTREVFEEKVILAGCEESGDFEAFKAANGDDKKAAEKACIALRTDGKSVDIDGDYTETLARIESNADGIGVFGLAFYENNTDKLQVATMSGVTPSTESISTGEYPVSRPLFFYVKKAHIGVIPGLKEYAEFFVSDEIAGPDGPLADYGLVADPELAKTQGVVADEVVMGGAS
>NZ_CP054599.1|WP_037921943.1|1975112_1976576_+|phosphate-ABC-transporter-permease-subunit-PstC
MVIVVLGLSALGFYMARSRAMASGNGDRRNLHSLPNYYGWNAAMLTAVPALGVLVIWLLAQPMMIDNAVFKTIPDSAIAEGSSRGLVMSDVRRLADGLDVIIQRGVMDAEQVTTLDAGTTDLRATLAAEGVALGSAVEQPVLDAAQMYRAQSSTGRFWMVALVTVIALAGFVLSLRVTNADFRARNMVEKGILTLLILSASIAILTTVGIVLSMLFESINFFRLHSWTDFFFGSTWAPNFRGDSDLSILPLLWGTLYISIIALLVAVPIGLFSAIYLSEYAGPKLRSAAKPLLEILAGIPTIVYGLFALLTVGPALVNIFGRGEQGLLGVEWMSGATSVLTAGLVMGIMLIPFVSSLSDDIINAVPQAMRDGSLGLGATHSETIRQVVLPAALPGIVGAILLAASRAIGETMIVVLGAGAIAKISANPLEAMTTITTRIVSQLTGDTNFASPETLVAFALGLTLFVLTLGLNVIALYIVRKYREQYD
>NZ_CP054599.1|WP_037920430.1|1976575_1977919_+|phosphate-ABC-transporter-permease-PstA
MTDMTSDTPHVHGTLLQADARTRKRNAAETRFKFYGILAIAVGLLMLAILLFNIVSRGTGAFTQTFVTLDVALLEDKLDKSGNRDIEEMKKVSTFGYAPLLKAAMTDMVEKTGMEGQISAGDMADILSKGAPAQLRDYVLANPDKVGQQVSFNFLASSRVDGYMKGRVSRDSIANDKSISAEQLDFVDKLIAQGILTKKFNLSFITGADASDARPEAAGMGVAMLGSFFMMLVVLVLALPIGVAASIYLEEFAPKNWITDIIEVNISNLAAVPSIVFGILGLAVFINYMHLPTSAPLVGGLVLTLMTLPTIIISTRASLKAVPPSIRDAALGVGASKMQSVFHHVLPLAAPGILTGTIIGLAQALGETAPLLLIGMVGFIASNAPDGIASGLLAPNSAMPAQIYEWAKRADPAFYERAWGGIIILLVFLLAMNTIAVILRRRFERRW
>NZ_CP054599.1|WP_037920432.1|1977937_1978732_+|phosphate-ABC-transporter-ATP-binding-protein
MKDLMAERTVDTQKLKISARNVQVYYGDTHAIKDVNVDIEDKTVTAFIGPSGCGKSTFLRCINRMNDTIDVCRVQGDIMIDGEDIYDKRVDPVQLRAKVGMVFQKPNPFPKSIYDNVAYGPRIHGLARNKADLDDIVERALRRGAIWNEVKDRLDAPGTGLSGGQQQRLCIARAVATEPEVLLMDEPCSALDPIATAQVEELIDELRQNYSVVIVTHSMQQAARVSQKTAFFHLGNLVEYGETGTIFTNPTDPRTESYITGRIG
>NZ_CP054599.1|WP_037920433.1|1978744_1979452_+|phosphate-signaling-complex-protein-PhoU
MPSDQHIASVFDRDLETIQAQIMKMGGLVEEAILEAAISLETRDEERAEAVRKADKKIDALEETINEEAARVIALRAPIAVDLRLILSVIKISANLERIGDYAKNMAKRTGVLAQLSPVSDSAGALRRMAREVERMLKDALDAYVNRDAELAQDVIDRDMEVDQMYNALFREFLTFMMEDPRNITACMHLHFIAKNVERMGDHVTSIAEQTVYLVTGVHADENRSKADTTSVQPL
>NZ_CP054599.1|WP_037920435.1|1979464_1980154_+|phosphate-regulon-transcriptional-regulator-PhoB
MSADQPRVLVVEDEPAQREVLVYNLSAEGFDVVSAGNGEDAMMMIDEASPDIIVLDWMMPHLSGIEVCRRLKIKPETRDIPVIMLSARSEEVDKVRGLETGADDYVVKPYSMIELMARVRSQLRRVRPATVGVQLHYEDIILDAERHRVTRGEAEIKLGPTEFRLLSTFMEKPGRVWSRESLLDRVWGRDIYVDTRTVDVHIGRLRKALTLSGGTDPVRTVRGAGYALG
>NZ_CP054599.1|WP_051693767.1|1980162_1980972_-|alpha/beta-hydrolase
MPPMRPDLPPPPGTRSFEDIAFGSHERQAIDVHLPPSGKINGVVANIHGGSWQWLDRKVIACWNLLDHGFAIVGMGYRMSDHAVFPAQICDINVGLAKLVQIAPDLDLPMDRVAVMGLSAGGHLAALAGLACDQPGFGPSHGIDIKGVIDIYGPSDLITLDGQWSNDPTRPPSAVTKLLGTSVTADPHHAYRASPVAHVTDQPAMPFLFIHGDEDTVVPLSHSLVLHAQLTANGTASFLKVIKGGGHATPDMHAPPVQEMIAKFLKKVF
>NZ_CP054599.1|WP_037920438.1|1980976_1981774_-|ABC-transporter-permease
MRTMSPLSRTLGIVFLLALPLPIFIVFGASFNGSAYIQFPPESYSLRWYIEAFTDPRWARAVGTSLWIAAVTSVVVTTISFMAAYFTLRYAPRINTMLELAIFSPLFFPHACMGVAAVALMAKLGIVGTPGAIILAHVIVTVPFSYRPVHVALRSVDPDLIRAAEVMGASELRALKDIILPICRSGILTSLLFTGIISFDEVTVTMFLIGPEITTLPVQIFTFIQDDASPVLAAISTISVIITFLAIVLLERVAGLTFFVQRSGS

You can click texts colored in the table to view more detailed information

Click the colored protein region to show detailed information

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP025986	Ralstonia solanacearum strain RSCM plasmid p-unname2, complete sequence	941294-941321	3	0.893
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP022367	Azospirillum sp. TSH58 plasmid TSH58_p02, complete sequence	1821284-1821311	5	0.821
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP033508	Mesorhizobium jarvisii strain ATCC 700743 plasmid pMJ700743a, complete sequence	131602-131629	5	0.821
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP033369	Mesorhizobium loti strain SU343 plasmid pMLSU343a, complete sequence	131602-131629	5	0.821
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP007795	Azospirillum brasilense strain Az39 plasmid AbAZ39_p2, complete sequence	619609-619636	5	0.821
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP016080	Mesorhizobium loti NZP2037 plasmid pML2037, complete sequence	358973-359000	5	0.821
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP016617	Microvirga ossetica strain V5/3m plasmid unnamed1, complete sequence	1281612-1281639	5	0.821
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP016618	Microvirga ossetica strain V5/3m plasmid unnamed3, complete sequence	49010-49037	5	0.821
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP030127	Indioceanicola profundi strain SCSIO 08040 plasmid unnamed1, complete sequence	269580-269607	6	0.786
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP054841	Acidovorax sp. 16-35-5 plasmid unnamed1, complete sequence	626222-626249	6	0.786
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	MK801723	Gordonia phage Coeur, complete genome	726-753	6	0.786
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	FR687252	Pantoea phage LIMElight complete genome	23786-23813	6	0.786
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NC_019454	Pantoea phage LIMElight, complete genome	23786-23813	6	0.786
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP054618	Azospirillum oryzae strain KACC 14407 plasmid unnamed4, complete sequence	373527-373554	7	0.75
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP049158	Caballeronia sp. SBC1 plasmid pSBC1_2, complete sequence	1484775-1484802	7	0.75
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP049318	Caballeronia sp. SBC2 plasmid pSBC2-2, complete sequence	1489443-1489470	7	0.75
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NC_007959	Nitrobacter hamburgensis X14 plasmid 1, complete sequence	223997-224024	7	0.75
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP016452	Sinorhizobium sp. RAC02 plasmid pBSY16_1, complete sequence	679734-679761	7	0.75
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NC_010510	Methylobacterium radiotolerans JCM 2831 plasmid pMRAD01, complete sequence	283611-283638	7	0.75
NZ_CP054599_2	2.1\|1971021\|28\|NZ_CP054599\|CRISPRCasFinder	1971021-1971048	28	NZ_CP016452	Sinorhizobium sp. RAC02 plasmid pBSY16_1, complete sequence	1790945-1790972	8	0.714

1. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP025986 (Ralstonia solanacearum strain RSCM plasmid p-unname2, complete sequence) position: , mismatch: 3, identity: 0.893

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
ccaccaggccatgctgcacggctgcacc	Protospacer
.*** ***.*******************

2. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP022367 (Azospirillum sp. TSH58 plasmid TSH58_p02, complete sequence) position: , mismatch: 5, identity: 0.821

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
cctggtggtcatgctgcacggctgcacc	Protospacer
.*  . **********************

3. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP033508 (Mesorhizobium jarvisii strain ATCC 700743 plasmid pMJ700743a, complete sequence) position: , mismatch: 5, identity: 0.821

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
cgacaaggtcatgctgcgcggctccagc	Protospacer
. ***************.***** ** *

4. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP033369 (Mesorhizobium loti strain SU343 plasmid pMLSU343a, complete sequence) position: , mismatch: 5, identity: 0.821

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
cgacaaggtcatgctgcgcggctccagc	Protospacer
. ***************.***** ** *

5. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP007795 (Azospirillum brasilense strain Az39 plasmid AbAZ39_p2, complete sequence) position: , mismatch: 5, identity: 0.821

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
cctcgtggtgatgctgcacggctgcacc	Protospacer
.* *. *** ******************

6. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP016080 (Mesorhizobium loti NZP2037 plasmid pML2037, complete sequence) position: , mismatch: 5, identity: 0.821

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
cgacaaggtcatgctgcgcggctccagc	Protospacer
. ***************.***** ** *

7. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP016617 (Microvirga ossetica strain V5/3m plasmid unnamed1, complete sequence) position: , mismatch: 5, identity: 0.821

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
gctcgtggtgatgctgcacggctgcacc	Protospacer
 * *. *** ******************

8. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP016618 (Microvirga ossetica strain V5/3m plasmid unnamed3, complete sequence) position: , mismatch: 5, identity: 0.821

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
gctcgtggtgatgctgcacggctgcacc	Protospacer
 * *. *** ******************

9. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP030127 (Indioceanicola profundi strain SCSIO 08040 plasmid unnamed1, complete sequence) position: , mismatch: 6, identity: 0.786

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
gctggtggtgatgctgcacggctgcacc	Protospacer
 *  . *** ******************

10. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP054841 (Acidovorax sp. 16-35-5 plasmid unnamed1, complete sequence) position: , mismatch: 6, identity: 0.786

tcacaa----ggtcatgctgcacggctgcacc	CRISPR spacer
----gatgctggtcatgctgcatggctgcacc	Protospacer
    .*    ************.*********

11. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to MK801723 (Gordonia phage Coeur, complete genome) position: , mismatch: 6, identity: 0.786

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
gtcctcggtcatgctgcaccgctgcacc	Protospacer
 . *  ************* ********

12. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to FR687252 (Pantoea phage LIMElight complete genome) position: , mismatch: 6, identity: 0.786

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
ttgcaatgtcattctgcacggctgcaag	Protospacer
*..*** ***** *************

13. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NC_019454 (Pantoea phage LIMElight, complete genome) position: , mismatch: 6, identity: 0.786

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
ttgcaatgtcattctgcacggctgcaag	Protospacer
*..*** ***** *************

14. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP054618 (Azospirillum oryzae strain KACC 14407 plasmid unnamed4, complete sequence) position: , mismatch: 7, identity: 0.75

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
gttggtggtgatgctgcacggctgcacc	Protospacer
 .  . *** ******************

15. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP049158 (Caballeronia sp. SBC1 plasmid pSBC1_2, complete sequence) position: , mismatch: 7, identity: 0.75

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
gctggtggtcatgctgcatggctgcacg	Protospacer
 *  . ************.********

16. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP049318 (Caballeronia sp. SBC2 plasmid pSBC2-2, complete sequence) position: , mismatch: 7, identity: 0.75

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
gctggtggtcatgctgcatggctgcacg	Protospacer
 *  . ************.********

17. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NC_007959 (Nitrobacter hamburgensis X14 plasmid 1, complete sequence) position: , mismatch: 7, identity: 0.75

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
gctggtcgtcatgcttcacggctgcacc	Protospacer
 *  .  ******** ************

18. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP016452 (Sinorhizobium sp. RAC02 plasmid pBSY16_1, complete sequence) position: , mismatch: 7, identity: 0.75

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
cctggtggtcgtgctgcacggctgcacg	Protospacer
.*  . ****.****************

19. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NC_010510 (Methylobacterium radiotolerans JCM 2831 plasmid pMRAD01, complete sequence) position: , mismatch: 7, identity: 0.75

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
cgtcgtggtgatgctgcacggctgcacg	Protospacer
.  *. *** *****************

20. spacer 2.1|1971021|28|NZ_CP054599|CRISPRCasFinder matches to NZ_CP016452 (Sinorhizobium sp. RAC02 plasmid pBSY16_1, complete sequence) position: , mismatch: 8, identity: 0.714

tcacaaggtcatgctgcacggctgcacc	CRISPR spacer
cttggtggtcgtgctgcacggctgcacg	Protospacer
..  . ****.****************

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

1621699 : 1633975

Paracoccus_phage(37.5%)

protease,portal,tail,capsid,head

The bacterium proteins that are colored denote the protein is present at specific phage-related keywords (such as 'capsid', 'head', 'integrase', 'plate', 'tail', 'fiber', 'coat', 'transposase', 'portal', 'terminase', 'protease' or 'lysin' and 'tRNA')

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037929310.1\|1621699_1622884_+\|portal	phage portal protein	W8ECU7	Geobacillus_phage	9.7e-61	36.2
WP_037929313.1\|1622876_1623101_+	hypothetical protein	NA	NA	NA	NA
WP_037929316.1\|1623124_1623688_+\|head,protease	HK97 family phage prohead protease	A0A0U2BX10	Paracoccus_phage	1.8e-33	52.6
WP_037929319.1\|1623724_1624903_+\|capsid	phage major capsid protein	B0VK33	Azospirillum_phage	2.9e-65	41.5
WP_037929320.1\|1625067_1625667_+\|head,tail	phage head-tail connector protein	NA	NA	NA	NA
WP_037929321.1\|1625663_1626002_+\|head,tail	head-tail adaptor protein	NA	NA	NA	NA
WP_037929323.1\|1625998_1626409_+	DUF3168 domain-containing protein	NA	NA	NA	NA
WP_037929326.1\|1626484_1626898_+\|tail	phage major tail protein, TP901-1 family	NA	NA	NA	NA
WP_037929332.1\|1626904_1627222_+	gene transfer agent family protein	NA	NA	NA	NA
WP_081855443.1\|1627230_1627422_+\|tail	phage tail assembly chaperone	NA	NA	NA	NA
WP_037929339.1\|1627418_1628081_+\|tail	phage tail tape measure protein	D6PFD6	uncultured_phage	3.9e-11	30.5
WP_037929479.1\|1628094_1628727_+	DUF2460 domain-containing protein	A0A0B5A2K3	Paracoccus_phage	1.4e-53	48.1
WP_037929341.1\|1628726_1629614_+	DUF2163 domain-containing protein	A0A0K0PVK1	Roseobacter_phage	1.7e-57	39.7
WP_037929346.1\|1629610_1630051_+	C40 family peptidase	F4YXU4	Roseobacter_phage	2.5e-30	45.7
WP_037929348.1\|1630051_1633975_+\|tail	glycoside hydrolase/phage tail family protein	A0A0B5A7K5	Paracoccus_phage	2.6e-227	37.9

DBSCAN-SWA_2

1855175 : 1882466

Paracoccus_phage(28.57%)

terminase,portal,integrase,transposase,capsid,head

attL 1854504:1854547|attR 1888813:1888856

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
1854504:1854547	attL	NA	NA	NA	NA
WP_143186824.1\|1855175_1856263_-\|transposase	IS3 family transposase	S5WIU1	Leptospira_phage	5.1e-48	39.8
WP_037920175.1\|1856436_1857510_-\|integrase	site-specific integrase	A0A1S5NNJ1	Burkholderia_phage	1.7e-19	29.4
WP_073194130.1\|1857398_1857677_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_037920178.1\|1857678_1857945_-	hypothetical protein	NA	NA	NA	NA
WP_037920181.1\|1858196_1860035_-	ParB N-terminal domain-containing protein	NA	NA	NA	NA
WP_037920183.1\|1860048_1860297_-	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_037920185.1\|1860293_1860596_-	hypothetical protein	NA	NA	NA	NA
WP_037920187.1\|1860592_1860808_-	hypothetical protein	NA	NA	NA	NA
WP_037920190.1\|1860873_1861143_+	hypothetical protein	NA	NA	NA	NA
WP_037920193.1\|1861152_1861443_-	hypothetical protein	NA	NA	NA	NA
WP_037920195.1\|1861439_1861730_-	hypothetical protein	NA	NA	NA	NA
WP_051693738.1\|1861950_1862259_-	transcriptional regulator	NA	NA	NA	NA
WP_162179359.1\|1862771_1862996_+	hypothetical protein	A0A1X9HW32	Ruegeria_phage	2.0e-07	45.7
WP_037920199.1\|1863393_1863885_+	hypothetical protein	NA	NA	NA	NA
WP_143186667.1\|1863881_1864658_+	methyltransferase	A0A076GD02	Sinorhizobium_phage	6.0e-51	50.6
WP_051693748.1\|1864657_1865506_+	ParB N-terminal domain-containing protein	A0A0U2C144	Paracoccus_phage	1.6e-36	36.6
WP_143186666.1\|1865508_1866069_+	hypothetical protein	NA	NA	NA	NA
WP_037920204.1\|1866061_1866346_+	hypothetical protein	NA	NA	NA	NA
WP_073194126.1\|1866369_1867047_+	helix-turn-helix domain-containing protein	NA	NA	NA	NA
WP_051693751.1\|1867043_1867646_+	hypothetical protein	NA	NA	NA	NA
WP_037920208.1\|1868405_1868810_+	HNH endonuclease	A0A2D1GNP5	Pseudomonas_phage	8.2e-20	45.4
WP_174861663.1\|1869198_1869960_+	Hint domain-containing protein	NA	NA	NA	NA
WP_174861664.1\|1870292_1870730_+\|terminase	P27 family phage terminase small subunit	NA	NA	NA	NA
WP_037920211.1\|1870716_1872384_+\|terminase	terminase	C7BGG7	Burkholderia_phage	1.1e-91	37.0
WP_143186665.1\|1872380_1873646_+\|portal	phage portal protein	E4ZFM3	Streptococcus_phage	1.3e-18	22.9
WP_037920214.1\|1873649_1874558_+	S49 family peptidase	A0A1B0Z1G3	Shewanella_phage	3.3e-08	32.0
WP_037920217.1\|1874647_1875961_+\|capsid	phage major capsid protein	Q6UAX6	Klebsiella_phage	2.8e-53	36.8
WP_037920221.1\|1876049_1876454_+	hypothetical protein	NA	NA	NA	NA
WP_037920224.1\|1876465_1877029_+	hypothetical protein	NA	NA	NA	NA
WP_037915382.1\|1877028_1877364_+\|head	phage head closure protein	NA	NA	NA	NA
WP_037920227.1\|1877360_1877810_+	HK97 gp10 family phage protein	A0A0U2C0P4	Paracoccus_phage	7.5e-22	42.6
WP_037920229.1\|1877813_1878197_+	DUF3168 domain-containing protein	A0A0U2BX31	Paracoccus_phage	7.8e-20	46.4
WP_037920231.1\|1878206_1878644_+	hypothetical protein	A0A2P1N0H6	Streptomyces_phage	2.5e-06	27.1
WP_037920233.1\|1878640_1879018_+	hypothetical protein	NA	NA	NA	NA
WP_037920235.1\|1879098_1879314_+	hypothetical protein	NA	NA	NA	NA
WP_051693755.1\|1879313_1882466_+	hypothetical protein	A0A0U2BXT9	Paracoccus_phage	4.3e-07	25.0
1888813:1888856	attR	NA	NA	NA	NA

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

2. NZ_CP054604

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_ID	Protospacer_location	Mismatch	Identity

MGE targeting detection<

CRISPR_ID	Spacer_Info	Spacer_region	Spacer_length	Hit_phage_ID	Hit_phage_def	Protospacer_location	Mismatch	Identity

Prophage detection

Region

Region Position

Protein_number

Hit_taxonomy

Key_proteins

Att_site

Prophage annotation

DBSCAN-SWA_1

0 : 10147

Ochrobactrum_phage(40.0%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037930867.1\|649_1135_-	hypothetical protein	NA	NA	NA	NA
WP_037930870.1\|1472_1766_+	hypothetical protein	NA	NA	NA	NA
WP_037930873.1\|1794_2151_-	hypothetical protein	NA	NA	NA	NA
WP_037930905.1\|2203_2473_-	type II toxin-antitoxin system Phd/YefM family antitoxin	NA	NA	NA	NA
WP_037930876.1\|2535_2748_-	hypothetical protein	NA	NA	NA	NA
WP_037930908.1\|3162_4212_-	replication protein C	L7TKN6	Rhizobium_phage	3.1e-10	26.7
WP_037930879.1\|4367_5438_-	plasmid partitioning protein RepB	A0A240F4U0	Ochrobactrum_phage	7.0e-26	34.5
WP_037930882.1\|5437_6625_-	plasmid partitioning protein RepA	A0A240F4U1	Ochrobactrum_phage	9.3e-88	47.1
WP_037930884.1\|6907_7789_-	recombinase family protein	A0A0C4UR34	Shigella_phage	1.1e-32	54.7
WP_114273239.1\|8015_9083_+	hypothetical protein	NA	NA	NA	NA
WP_037931786.1\|9142_10147_-\|transposase	IS30 family transposase	W5R8L2	Staphylococcus_phage	2.0e-38	33.4

DBSCAN-SWA_2

32830 : 34845

Bacillus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037931853.1\|32830_34183_-	two-component sensor histidine kinase	W8CYF6	Bacillus_phage	2.9e-08	23.5
WP_037239933.1\|34179_34845_-	response regulator transcription factor	W8CYM9	Bacillus_phage	5.5e-21	28.3

DBSCAN-SWA_3

39042 : 39753

Streptococcus_phage(100.0%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037932195.1\|39042_39753_+\|transposase	IS6 family transposase	A0A1X9I6C6	Streptococcus_phage	5.0e-28	33.8

DBSCAN-SWA_4

52142 : 52601

Acinetobacter_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037931531.1\|52142_52601_+	(2Fe-2S)-binding protein	A0A0P0IVM8	Acinetobacter_phage	5.5e-12	35.4

DBSCAN-SWA_5

56925 : 63327

Chrysochromulina_ericina_virus(25.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037931540.1\|56925_57780_+	SDR family oxidoreductase	A0A0N9R355	Chrysochromulina_ericina_virus	3.6e-17	25.0
WP_037931568.1\|57821_58625_+	ABC transporter ATP-binding protein	A0A2H4PQG7	Staphylococcus_phage	2.1e-14	25.3
WP_037931543.1\|58602_60537_+	AMP-binding protein	A0A1V0SBX8	Catovirus	1.2e-39	22.0
WP_037931546.1\|60537_61425_+	branched-chain amino acid ABC transporter permease	NA	NA	NA	NA
WP_037931550.1\|61428_62502_+	branched-chain amino acid ABC transporter permease	NA	NA	NA	NA
WP_037931553.1\|62514_63327_+	ABC transporter ATP-binding protein	W6JKT0	Anomala_cuprea_entomopoxvirus	2.2e-11	24.3

DBSCAN-SWA_6

83241 : 84018

Prochlorococcus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037929932.1\|83241_84018_-	SDR family oxidoreductase	A0A0K0KVL6	Prochlorococcus_phage	4.0e-07	30.4

DBSCAN-SWA_7

119497 : 120289

Brazilian_cedratvirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037929841.1\|119497_120289_-	ABC transporter ATP-binding protein	A0A2R8FG22	Brazilian_cedratvirus	3.1e-10	25.3

DBSCAN-SWA_8

123579 : 126367

Catovirus(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037929830.1\|123579_125556_-	AMP-binding protein	A0A1V0SBX8	Catovirus	3.0e-22	18.6
WP_081855452.1\|125581_126367_-	ABC transporter ATP-binding protein	G3M9Y6	Bacillus_virus	2.1e-11	21.6

DBSCAN-SWA_9

130316 : 134905

Klosneuvirus(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037929818.1\|130316_131276_-	hydroxymethylglutaryl-CoA lyase	A0A1V0SKU2	Klosneuvirus	5.0e-23	31.2
WP_037929816.1\|131280_133287_-	biotin/lipoyl-binding protein	NA	NA	NA	NA
WP_037929814.1\|133297_134905_-	methylcrotonoyl-CoA carboxylase	A0A1B2ITV7	Pike_perch_iridovirus	4.6e-13	48.6

DBSCAN-SWA_10

141598 : 143134

Staphylococcus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037929804.1\|141598_143134_+	AMP-binding protein	A0A2H4PQM9	Staphylococcus_phage	5.5e-32	26.5

DBSCAN-SWA_11

146320 : 147082

Diadromus_pulchellus_ascovirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037929794.1\|146320_147082_-	glucose 1-dehydrogenase	F2NZ40	Diadromus_pulchellus_ascovirus	2.9e-18	32.7

DBSCAN-SWA_12

150252 : 151107

Trichoplusia_ni_ascovirus(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037929778.1\|150252_151107_-	SDR family oxidoreductase	Q06VL0	Trichoplusia_ni_ascovirus	3.4e-07	24.3

DBSCAN-SWA_13

157591 : 163081

Pike_perch_iridovirus(66.67%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037931808.1\|157591_159199_+	methylcrotonoyl-CoA carboxylase	A0A1B2ITV7	Pike_perch_iridovirus	3.5e-13	58.7
WP_037931805.1\|159343_160528_+	lipid-transfer protein	NA	NA	NA	NA
WP_037931802.1\|160552_161383_+	SDR family oxidoreductase	Q06VL0	Trichoplusia_ni_ascovirus	4.0e-13	26.6
WP_037931799.1\|161482_163081_+	methylcrotonoyl-CoA carboxylase	A0A1B2ITV7	Pike_perch_iridovirus	6.2e-10	43.1

DBSCAN-SWA_14

168048 : 172306

Staphylococcus_phage(50.0%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037931786.1\|168048_169053_+\|transposase	IS30 family transposase	W5R8L2	Staphylococcus_phage	2.0e-38	33.4
WP_073194621.1\|169390_169912_+	hypothetical protein	NA	NA	NA	NA
WP_024099346.1\|170032_170260_-	hypothetical protein	NA	NA	NA	NA
WP_037931778.1\|170429_170687_-	helix-turn-helix transcriptional regulator	NA	NA	NA	NA
WP_037931775.1\|171273_171642_+	hypothetical protein	NA	NA	NA	NA
WP_037931772.1\|171685_172306_+	lytic transglycosylase domain-containing protein	A0A1P8CWQ1	Bacillus_phage	2.2e-19	53.5

DBSCAN-SWA_15

175589 : 176744

Bacillus_phage(100.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_174861788.1\|175589_176744_+	lytic transglycosylase domain-containing protein	A0A1P8CWQ1	Bacillus_phage	1.1e-27	57.7

DBSCAN-SWA_16

184764 : 186041

Wolbachia_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037930778.1\|184764_185802_-	patatin-like phospholipase family protein	A0A1B2LRS3	Wolbachia_phage	3.1e-18	30.7
WP_009815559.1\|185816_186041_-	DUF2188 domain-containing protein	E8ZD70	Streptococcus_phage	9.8e-15	56.9

DBSCAN-SWA_17

193941 : 197944

uncultured_Mediterranean_phage(33.33%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_081855475.1\|193941_195147_-	SH3 domain-containing protein	A0A1B1IWW0	uncultured_Mediterranean_phage	8.2e-07	36.5
WP_037930798.1\|195249_196179_-	DUF2493 domain-containing protein	A0A291L9X7	Bordetella_phage	8.3e-07	33.0
WP_037930801.1\|196861_197944_+	ATP-binding protein	A0A2K9R7H3	Dishui_lake_phycodnavirus	6.4e-19	28.8

DBSCAN-SWA_18

201738 : 206972

uncultured_Mediterranean_phage(50.0%)

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037930810.1\|201738_202200_-	hypothetical protein	A0A1B1IUL8	uncultured_Mediterranean_phage	1.8e-31	55.2
WP_037930812.1\|202201_202588_-	hypothetical protein	NA	NA	NA	NA
WP_037930815.1\|202709_206972_-	strawberry notch family protein	A0A1X9HW54	Ruegeria_phage	1.3e-11	29.7

DBSCAN-SWA_19

217414 : 218501

Leptospira_phage(100.0%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_143186824.1\|217414_218501_+\|transposase	IS3 family transposase	S5WIU1	Leptospira_phage	5.1e-48	39.8

DBSCAN-SWA_20

225948 : 231949

uncultured_Caudovirales_phage(33.33%)

transposase

Protein_ID	Protein_Def	Hit_ID	Hit_Def	E-value	Identity
WP_037930848.1\|225948_226800_-	helix-turn-helix domain-containing protein	A0A2H4J8A6	uncultured_Caudovirales_phage	1.1e-21	36.8
WP_037930850.1\|227029_227686_-	hypothetical protein	NA	NA	NA	NA
WP_037930853.1\|227685_228279_-	hypothetical protein	NA	NA	NA	NA
WP_037930856.1\|228351_230334_-	ParB N-terminal domain-containing protein	A0A0F7L836	uncultured_marine_virus	3.0e-06	33.5
WP_037930859.1\|230908_231949_-\|transposase	IS110 family transposase	A0A1S7J231	Thermus_phage	7.1e-07	26.7

Anti-CRISPR protein detection

Acr ID	Acr position	Acr size	Homology with known anti	Neighbor HTH/AcRanker	Neighbor Aca	In prophage	Protospacer in prophage

Overview of predicted results

Overview of the results

Cas Category Instructions

Results visualization

1. NZ_CP054599

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Click the colored protein region to show detailed information

Click the colored protein region to show detailed information

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection

2. NZ_CP054604

Click the left colored region to show detailed information

CRISPR-Cas detection and classification

Self-targeting detection

MGE targeting detection<

Prophage detection

Anti-CRISPR protein detection